Q9SJE9 · ALMT1_ARATH
- ProteinAluminum-activated malate transporter 1
- GeneALMT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids493 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Malate transporter critical for aluminum tolerance. The STOP1 transcription factor is required for ALMT1 expression.
Miscellaneous
Essential factor for aluminum (Al) tolerance but does not represent the major Al tolerance QTL also found on chromosome 1. Acts in parallel but independently of MATE, an aluminum-activated root citrate transporter.
A reversible phosphorylation acting both at the transcriptional and post-transcriptional levels is required to activate malate release in response to aluminum.
Activity regulation
Activated by external aluminum.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | malate transmembrane transporter activity | |
Biological Process | malate transmembrane transport | |
Biological Process | monoatomic ion transmembrane transport | |
Biological Process | response to aluminum ion |
Keywords
- Molecular function
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameAluminum-activated malate transporter 1
- Short namesAtALMT1
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SJE9
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 28-48 | Helical | ||||
Sequence: VGLALVLVSSFYYYQPFGPFT | ||||||
Transmembrane | 51-71 | Helical | ||||
Sequence: FGINAMWAVMTVVVVFEFSVG | ||||||
Transmembrane | 104-124 | Helical | ||||
Sequence: TVEPILLVMLVFVQAALSTFV | ||||||
Transmembrane | 133-153 | Helical | ||||
Sequence: KFDYGILIFILTFALISLSGF | ||||||
Transmembrane | 169-189 | Helical | ||||
Sequence: VVIGGVSCILISIFVCPVWAG |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible phenotype when grown under normal conditions. Lack of aluminum-activated malate release and shorter roots when submitted to aluminum stress.
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 33-36 | in strain: cv. Nd-0, cv. No-0 | ||||
Sequence: VLVS → GLVA | ||||||
Natural variant | 36 | in strain: cv. Bay-0, cv. Landsberg erecta | ||||
Sequence: S → A | ||||||
Natural variant | 77-493 | in strain: cv. Wa-1; induces hypersensitivity to aluminum stress | ||||
Sequence: Missing | ||||||
Natural variant | 194 | in strain: cv. Bay-0 | ||||
Sequence: S → F | ||||||
Natural variant | 257 | in strain: cv. Landsberg erecta | ||||
Sequence: P → S | ||||||
Natural variant | 298 | in strain: cv. Bay-0, cv. Cvi-0, cv. Landsberg erecta, cv. Nd-0, cv. No-0 | ||||
Sequence: I → V | ||||||
Natural variant | 375 | in strain: cv. Cvi-0 | ||||
Sequence: V → I |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 44 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000401460 | 1-493 | Aluminum-activated malate transporter 1 | |||
Sequence: MEKVREIVREGIRVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGATLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKTKFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDLHSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDSKSDEEALANYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLRRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILNDVEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPTVLYEKSDSGSIGRAMPIDSHEDHHVVTVLHDVDNDRSNNVDDSRGGSSQDSCHHVAIKIVDDNSNHEKHEDGEIHVHTLSNGHLQ | ||||||
Modified residue | 320 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 327 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 385 | Phosphothreonine | ||||
Sequence: T |
Post-translational modification
Phosphorylated. A reversible phosphorylation is required for activation (By similarity).
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Expressed in roots, but not in shoots. Detected in the root apex in absence of aluminum stress and in root apices, the stele and endodermis of the elongating zone of primary and lateral roots after aluminum stress. Not expressed in cortical and epidermal cells.
Induction
Up-regulated by aluminum. Small induction by erbium (Er) and low pH stress, but no induction by cadmium, copper, lanthanum or sodium.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 441-460 | Disordered | ||||
Sequence: DNDRSNNVDDSRGGSSQDSC |
Sequence similarities
Belongs to the aromatic acid exporter (TC 2.A.85) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length493
- Mass (Da)55,077
- Last updated2000-05-01 v1
- ChecksumCAEBF439C3E5CEC6
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ465038 EMBL· GenBank· DDBJ | ABF22742.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ465039 EMBL· GenBank· DDBJ | ABF22743.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ465040 EMBL· GenBank· DDBJ | ABF22744.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ465041 EMBL· GenBank· DDBJ | ABF22745.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ465042 EMBL· GenBank· DDBJ | ABF22746.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC006932 EMBL· GenBank· DDBJ | AAF22890.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE28289.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU181365 EMBL· GenBank· DDBJ | ABW33503.1 EMBL· GenBank· DDBJ | mRNA |