Q9SJC4 · NUDT6_ARATH
- ProteinNudix hydrolase 6
- GeneNUDT6
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids283 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Probably mediates the hydrolysis of some nucleoside diphosphate derivatives. In vitro, it can use both NADH and ADP-ribose as substrates; however the relevance of such substrates in vivo is unclear.
Catalytic activity
- ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate + 2 H+
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Divalent metal cations. Mg2+ or Mn2+.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
23 μM | ADP-ribose | |||||
13.7 μM | NADH |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
0.18 μmol/min/mg | with ADP-ribose as substrate | ||||
0.32 μmol/min/mg | with NADPH as substrate |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ADP-ribose diphosphatase activity | |
Molecular Function | metal ion binding | |
Molecular Function | NAD binding | |
Molecular Function | NAD+ diphosphatase activity | |
Molecular Function | NADH pyrophosphatase activity | |
Biological Process | positive regulation of salicylic acid mediated signaling pathway | |
Biological Process | response to other organism |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNudix hydrolase 6
- EC number
- Short namesAtNUDT6
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SJC4
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000057126 | 1-283 | Nudix hydrolase 6 | |||
Sequence: MDNEDQESLLLQGVPDNYGGVKVNLTEPMTIEDFVPKLRASLVYWSNQGTKGIWLKLADGLDNLIAPAKAEGFVCHHAEREYTMLTSWIADVPSTLPANASHRIGVGAFVLNKKTKEVLVVQEIDGHFKGTGVWKLPTGVVKEGENIWEGALREVEEETGIKTKFVEVLAFRESHQAFLEIKTDIFFLCELEPTTFEIKKQDSEILAAKWMPIEEYVNQPWNQKKELFRFMANICLKRLQEMEYMGFSKVLTTTSSGKESYLYCNTDHANLLNATRGLASTSG |
Proteomic databases
Expression
Tissue specificity
Expressed in stems and leaves. Weakly or not expressed in roots.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 101-233 | Nudix hydrolase | ||||
Sequence: SHRIGVGAFVLNKKTKEVLVVQEIDGHFKGTGVWKLPTGVVKEGENIWEGALREVEEETGIKTKFVEVLAFRESHQAFLEIKTDIFFLCELEPTTFEIKKQDSEILAAKWMPIEEYVNQPWNQKKELFRFMAN | ||||||
Motif | 139-160 | Nudix box | ||||
Sequence: GVVKEGENIWEGALREVEEETG |
Sequence similarities
Belongs to the Nudix hydrolase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length283
- Mass (Da)31,913
- Last updated2000-05-01 v1
- ChecksumB8140657498E57B4
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC006951 EMBL· GenBank· DDBJ | AAD25833.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC05837.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CB185816 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |