Q9SIB9 · ACO3M_ARATH

Function

function

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Contributes to oxidative stress tolerance (PubMed:17013749).
Modulates cytosolic citrate metabolism during lipid mobilization. Required during seedling growth (PubMed:25061985).

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site182substrate
Binding site301-303substrate
Binding site533[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site599[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site602[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site632substrate
Binding site637substrate
Binding site795substrate
Binding site876-877substrate

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentchloroplast stroma
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentmitochondrion
Cellular Componentplant-type cell wall
Cellular Componentplant-type vacuole
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionaconitate hydratase activity
Molecular FunctionATP binding
Molecular Functioncopper ion binding
Biological Processcitrate metabolic process
Biological Processglyoxylate cycle
Biological Processisocitrate metabolic process
Biological Processresponse to abscisic acid
Biological Processresponse to oxidative stress
Biological Processseedling development
Biological Processtricarboxylic acid cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aconitate hydratase 3, mitochondrial
  • EC number
  • Short names
    Aconitase 3
    ; mACO1
  • Alternative names
    • Citrate hydro-lyase 3

Gene names

    • Name
      ACO3
    • ORF names
      T3P4.5
    • Ordered locus names
      At2g05710

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9SIB9
  • Secondary accessions
    • Q8L784

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Mitochondrion
Cytoplasm
Note: Cytosolic localization in 3-day-old seedlings, but mitochondrial in 10-day-old plantlets.

Keywords

Phenotypes & Variants

Disruption phenotype

Reduced cytosolic and mitochondrial aconitase (ACO) activities by 25 and 55 precent, respectively (PubMed:17013749, PubMed:17437406).
Increased tolerance to oxidative stress mediated by paraquat, a superoxide-generating agent (PubMed:17013749).
Delayed early seedling growth, altered assimilation of acetate feeding and elevated citrate and malate levels (PubMed:25061985).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 57 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain, modified residue.

TypeIDPosition(s)Description
Transit peptide1-78Mitochondrion
ChainPRO_000025992179-990Aconitate hydratase 3, mitochondrial
Modified residue91Phosphoserine

Post-translational modification

Phosphorylated at Ser-91 in the cytoplasm; this phosphorylation requires the presence of B'GAMMA.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Major aconitase isoenzyme in young seedlings (PubMed:25061985).
Expressed in roots, leaves, stems and flowers, and, at low levels, in seeds (PubMed:17437406).

Induction

Transiently induced during germination.

Gene expression databases

Interaction

Subunit

Monomer (By similarity).
Interacts with B'GAMMA in the cytosol (PubMed:25307043).

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the aconitase/IPM isomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    990
  • Mass (Da)
    108,201
  • Last updated
    2006-11-28 v2
  • Checksum
    552AE563BC2981ED
MYLTASSSASSSIIRAASSRSSSLFSFRSVLSPSVSSTSPSSLLARRSFGTISPAFRRWSHSFHSKPSPFRFTSQIRAVSPVLDRLQRTFSSMASEHPFKGIFTTLPKPGGGEFGKFYSLPALNDPRVDKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSTAFQNMLVVPPGSGIVHQVNLEYLGRVVFNTKGLLYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLAGKMRNGVTATDLVLTVTQMLRKHGVVGKFVEFYGNGMSGLSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAMIEAYLRANNMFVDYNEPQQDRVYSSYLELNLDDVEPCISGPKRPHDRVTLKEMKADWHSCLDSKVGFKGFAIPKEAQEKVVNFSFDGQPAELKHGSVVIAAITSCTNTSNPSVMLGAGLVAKKACDLGLQVKPWIKTSLAPGSGVVTKYLLKSGLQEYLNEQGFNIVGYGCTTCIGNSGEINESVGAAITENDIVAAAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVNIDFETEPIGKGKNGKDVFLRDIWPTTEEIAEVVQSSVLPDMFRATYESITKGNPMWNKLSVPENTLYSWDPNSTYIHEPPYFKDMTMDPPGPHNVKDAYCLLNFGDSITTDHISPAGNIQKDSPAAKFLMERGVDRKDFNSYGSRRGNDEIMARGTFANIRIVNKLMNGEVGPKTVHIPSGEKLSVFDAAMRYKSSGEDTIILAGAEYGSGSSRDWAAKGPMLQGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADTLGLTGHERYTIHLPTDISEIRPGQDVTVTTDNGKSFTCTVRFDTEVELAYFNHGGILPYVIRNLSKQ

Sequence caution

The sequence AAD25640.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC007170
EMBL· GenBank· DDBJ
AAD25640.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
CP002685
EMBL· GenBank· DDBJ
AEC05964.1
EMBL· GenBank· DDBJ
Genomic DNA
AY136414
EMBL· GenBank· DDBJ
AAM97080.1
EMBL· GenBank· DDBJ
mRNA
BT008809
EMBL· GenBank· DDBJ
AAP68248.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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