Q9SHJ5 · GPAT1_ARATH
- ProteinGlycerol-3-phosphate acyltransferase 1
- GeneGPAT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids585 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Involved in pollen development, by being required for tapetum differentiation and male fertility. In addition to the sporophytic effect, it also exerts a gametophytic effect on pollen performance.
Catalytic activity
- an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + CoA
Kinetics
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
217.42 pmol/min/mg |
Pathway
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | mitochondrion | |
Molecular Function | glycerol-3-phosphate 2-O-acyltransferase activity | |
Molecular Function | glycerol-3-phosphate O-acyltransferase activity | |
Molecular Function | sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity | |
Biological Process | CDP-diacylglycerol biosynthetic process | |
Biological Process | pollen sperm cell differentiation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlycerol-3-phosphate acyltransferase 1
- EC number
- Short namesAtGPAT1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SHJ5
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 126-146 | Helical | ||||
Sequence: FFPYFMLVAFEGGSIIRAILL | ||||||
Transmembrane | 334-354 | Helical | ||||
Sequence: TPLATLAMFIWLPIGFLLAVF | ||||||
Transmembrane | 356-376 | Helical | ||||
Sequence: ISVGVFLPYHVANFLASMSGV |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Plants display a massive pollen development arrest due to a perturbed degeneration of the tapetum, which is associated with altered endoplasmic reticulum profiles and reduced secretion. Defects correlate with several fatty acid composition changes in flower tissues and seeds. However, no significant change in seed oil content is observed.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 28 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000195249 | 1-585 | Glycerol-3-phosphate acyltransferase 1 | |||
Sequence: MVLPELLVILAEWVLYRLLAKSCYRAARKLRGYGFQLKNLLSLSKTQSLHNNSQHHLHNHHQQNHPNQTLQDSLDPLFPSLTKYQELLLDKNRACSVSSDHYRDTFFCDIDGVLLRQHSSKHFHTFFPYFMLVAFEGGSIIRAILLLLSCSFLWTLQQETKLRVLSFITFSGLRVKDMDNVSRSVLPKFFLENLNIQVYDIWARTEYSKVVFTSLPQVLVERFLREHLNADDVIGTKLQEIKVMGRKFYTGLASGSGFVLKHKSAEDYFFDSKKKPALGIGSSSSPQDHIFISICKEAYFWNEEESMSKNNALPRERYPKPLIFHDGRLAFLPTPLATLAMFIWLPIGFLLAVFRISVGVFLPYHVANFLASMSGVRITFKTHNLNNGRPEKGNSGVLYVCNHRTLLDPVFLTTSLGKPLTAVTYSLSKFSEFIAPLKTVSLKRDRKKDGEAMQRLLSKGDLVVCPEGTTCREPYLLRFSPLFAELTEDIVPVAVDARVSMFYGTTASGLKCLDPIFFLMNPRPVYCLEILKKLPKEMTCAGGKSSFEVANFIQGELARVLGFECTNLTRRDKYLVLAGNEGIVR |
Proteomic databases
Expression
Tissue specificity
Highly expressed in developing siliques and flower buds. Weakly or not expressed in roots, seedlings and leaves.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 403-408 | HXXXXD motif | ||||
Sequence: HRTLLD |
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Sequence similarities
Belongs to the GPAT/DAPAT family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length585
- Mass (Da)66,514
- Last updated2000-05-01 v1
- Checksum3F7FDCE161C28EAF
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 21 | in Ref. 3; AAM67097 | ||||
Sequence: K → N | ||||||
Sequence conflict | 270 | in Ref. 3; AAM67097 | ||||
Sequence: F → L |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC007592 EMBL· GenBank· DDBJ | AAF24816.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE28001.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY088785 EMBL· GenBank· DDBJ | AAM67097.1 EMBL· GenBank· DDBJ | mRNA |