Q9SFH9 · HEM21_ARATH

Function

function

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site298Schiff-base intermediate with substrate
Binding site3085-aminolevulinate 1 (UniProtKB | ChEBI)
Binding site3205-aminolevulinate 1 (UniProtKB | ChEBI)
Binding site336Mg2+ (UniProtKB | ChEBI)
Active site351Schiff-base intermediate with substrate
Binding site3775-aminolevulinate 2 (UniProtKB | ChEBI)
Binding site4165-aminolevulinate 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentchloroplast stroma
Cellular Componentmitochondrion
Molecular Functionmetal ion binding
Molecular Functionporphobilinogen synthase activity
Biological Processchlorophyll biosynthetic process
Biological Processporphyrin-containing compound biosynthetic process
Biological Processprotoporphyrinogen IX biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Delta-aminolevulinic acid dehydratase 1, chloroplastic
  • EC number
  • Short names
    ALADH1
  • Alternative names
    • Porphobilinogen synthase

Gene names

    • Name
      HEMB1
    • ORF names
      T6C23.6
    • Ordered locus names
      At1g69740

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9SFH9

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Embryo lethal when homozygous. Impaired plant growth and development.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 26 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-52Chloroplast
ChainPRO_000001331453-430Delta-aminolevulinic acid dehydratase 1, chloroplastic

Proteomic databases

PTM databases

Expression

Tissue specificity

Highly expressed in cotyledons during dark-to-light transition.

Induction

Up-regulated by the transcription factors FAR1 and FHY3.

Gene expression databases

Interaction

Subunit

Homooctamer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region82-101Disordered

Sequence similarities

Belongs to the ALAD family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    430
  • Mass (Da)
    46,690
  • Last updated
    2000-05-01 v1
  • Checksum
    44B0984247FC6147
MATTPIFNASCSFPSTRGIDCKSYIGLRSNVSKVSVASSRIATSQRRNLVVRASESGNGHAKKLGMSDAECEAAVAAGNVPEAPPVPPKPAAPVGTPIIKPLNLSRRPRRNRASPVTRAAFQETDISPANFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLVQEVAKARAVGVNSIVLFPKVPEALKNSTGDEAYNDNGLVPRTIRLLKDKYPDLIIYTDVALDPYSSDGHDGIVREDGVIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRSALDAEGFQNVSIMSYTAKYASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIEAREDEAEGADILLVKPGLPYLDIIRLLRDKSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQAATCLCGEKR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC013289
EMBL· GenBank· DDBJ
AAG52549.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE34969.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE34970.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
ANM58523.1
EMBL· GenBank· DDBJ
Genomic DNA
AF327428
EMBL· GenBank· DDBJ
AAG42018.1
EMBL· GenBank· DDBJ
mRNA
AF361803
EMBL· GenBank· DDBJ
AAK32816.1
EMBL· GenBank· DDBJ
mRNA
AY059154
EMBL· GenBank· DDBJ
AAL15379.1
EMBL· GenBank· DDBJ
mRNA
AY081254
EMBL· GenBank· DDBJ
AAL91143.1
EMBL· GenBank· DDBJ
mRNA
AY113941
EMBL· GenBank· DDBJ
AAM44989.1
EMBL· GenBank· DDBJ
mRNA
AY120705
EMBL· GenBank· DDBJ
AAM53263.1
EMBL· GenBank· DDBJ
mRNA
AY128711
EMBL· GenBank· DDBJ
AAM91111.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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