Q9SEZ6 · RNH2A_ARATH
- ProteinRibonuclease H2 subunit A
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids296 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes (By similarity).
Catalytic activity
Cofactor
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Note: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | metal ion binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA-DNA hybrid ribonuclease activity | |
Biological Process | RNA metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonuclease H2 subunit A
- EC number
- Short namesRNase H2 subunit A
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SEZ6
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000111715 | 1-296 | Ribonuclease H2 subunit A | |||
Sequence: MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEEKREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVLLTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWLVEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWEADENEESGNGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF |
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 14-236 | RNase H type-2 | ||||
Sequence: PCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEEKREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVLLTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWLVEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVE |
Sequence similarities
Belongs to the RNase HII family. Eukaryotic subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q9SEZ6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length296
- Mass (Da)33,078
- Last updated2004-06-21 v2
- ChecksumBBC556D1CBED74CF
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A8MRI6 | A8MRI6_ARATH | At2g25100 | 277 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 64 | in Ref. 5; AAM65508 | ||||
Sequence: E → D | ||||||
Sequence conflict | 136 | in Ref. 5; AAM65508 | ||||
Sequence: R → K |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002685 EMBL· GenBank· DDBJ | AEC07657.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK117633 EMBL· GenBank· DDBJ | BAC42289.1 EMBL· GenBank· DDBJ | mRNA | ||
BT005597 EMBL· GenBank· DDBJ | AAO64017.1 EMBL· GenBank· DDBJ | mRNA | ||
AY087961 EMBL· GenBank· DDBJ | AAM65508.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |