Q9SEZ6 · RNH2A_ARATH

Function

function

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes (By similarity).

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.

Features

Showing features for binding site.

129620406080100120140160180200220240260280
TypeIDPosition(s)Description
Binding site20a divalent metal cation (UniProtKB | ChEBI)
Binding site21a divalent metal cation (UniProtKB | ChEBI)
Binding site127a divalent metal cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular FunctionRNA binding
Molecular FunctionRNA-DNA hybrid ribonuclease activity
Biological ProcessRNA metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease H2 subunit A
  • EC number
  • Short names
    RNase H2 subunit A
  • Alternative names
    • Ribonuclease HI large subunit (RNase HI large subunit)
    • Ribonuclease HI subunit A

Gene names

    • ORF names
      F13D4.60
    • Ordered locus names
      At2g25100

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9SEZ6
  • Secondary accessions
    • Q8GYG9
    • Q8LA95

Proteomes

Organism-specific databases

Genome annotation databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001117151-296Ribonuclease H2 subunit A

Proteomic databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain14-236RNase H type-2

Sequence similarities

Belongs to the RNase HII family. Eukaryotic subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q9SEZ6-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    296
  • Mass (Da)
    33,078
  • Last updated
    2004-06-21 v2
  • Checksum
    BBC556D1CBED74CF
MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEEKREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVLLTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWLVEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWEADENEESGNGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A8MRI6A8MRI6_ARATHAt2g25100277

Sequence caution

The sequence AAM65508.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict64in Ref. 5; AAM65508
Sequence conflict136in Ref. 5; AAM65508

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002685
EMBL· GenBank· DDBJ
AEC07657.1
EMBL· GenBank· DDBJ
Genomic DNA
AK117633
EMBL· GenBank· DDBJ
BAC42289.1
EMBL· GenBank· DDBJ
mRNA
BT005597
EMBL· GenBank· DDBJ
AAO64017.1
EMBL· GenBank· DDBJ
mRNA
AY087961
EMBL· GenBank· DDBJ
AAM65508.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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