Q9SBL1 · HMNGT_SORBI

Function

function

Involved in the biosynthesis of the cyanogenic glucoside dhurrin. Prevents the dissociation and release of toxic hydrogen cyanide. Mandelonitrile, p-hydroxymandelonitrile, benzyl alcohol, benzoic acid and geraniol, but not hydroquinone(1,4-benzenediol), alpha-terpinol, linalool or farnesol are utilized as acceptor substrates. UDP-glucose, but not UDP-xylose or UDP-glucuronic acid can be used as sugar donor.

Catalytic activity

Activity regulation

Inhibited by p-hydroxybenzaldehyde.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.14 mMgeraniol
0.13 mMbeta-citronellol
1.13 mMnerol
0.66 mM1-hexanol
0.73 mMcis-3-hexen-1-ol
0.84 mMmandelonitrile
6.33 mM2-hydroxy-3-methoxybenzyl alcohol
0.69 mM3-methyl-3-buten-1-ol
10.3 mM3-methyl-2-buten-1-ol
0.8 mMUDP-glucose

Pathway

Secondary metabolite biosynthesis; dhurrin biosynthesis; dhurrin from L-tyrosine: step 3/3.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site23Proton acceptor
Binding site23an anthocyanidin (UniProtKB | ChEBI)
Active site130Charge relay
Binding site152UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site371UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site386UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site389UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site390UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site391UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site394UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site409an anthocyanidin (UniProtKB | ChEBI)
Binding site410UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site411UDP-alpha-D-glucose (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functioncyanohydrin beta-glucosyltransferase activity
Biological Processdhurrin biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GT1Glycosyltransferase Family 1

Names & Taxonomy

Protein names

  • Recommended name
    Cyanohydrin beta-glucosyltransferase
  • EC number
  • Alternative names
    • UDP-glucose-p-hydroxymandelonitrile glucosyltransferase

Gene names

    • Name
      UGT85B1
    • Synonyms
      HMNGT

Organism names

  • Taxonomic identifier
  • Strain
    • cv. MR31
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Sorghinae > Sorghum

Accessions

  • Primary accession
    Q9SBL1

Genome annotation databases

Subcellular Location

Cytoplasm
Endoplasmic reticulum membrane
; Peripheral membrane protein
Note: In the presence of CYP79A1 and CYP71E1, moves toward the surface of the ER membranes in order to form a metabolon.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis20120-fold reduction in activity.
Mutagenesis391185-fold reduction in activity.
Mutagenesis410225-fold reduction in activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003656081-492Cyanohydrin beta-glucosyltransferase

Post-translational modification

The N-terminus is partially blocked.

Expression

Gene expression databases

Family & Domains

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    492
  • Mass (Da)
    52,916
  • Last updated
    2000-05-01 v1
  • Checksum
    ABC6A57AC51E9BE7
MGSNAPPPPTPHVVLVPFPGQGHVAPLMQLARLLHARGARVTFVYTQYNYRRLLRAKGEAAVRPPATSSARFRIEVIDDGLSLSVPQNDVGGLVDSLRKNCLHPFRALLRRLGQEVEGQDAPPVTCVVGDVVMTFAAAAAREAGIPEVQFFTASACGLLGYLHYGELVERGLVPFRDASLLADDDYLDTPLEWVPGMSHMRLRDMPTFCRTTDPDDVMVSATLQQMESAAGSKALILNTLYELEKDVVDALAAFFPPIYTVGPLAEVIASSDSASAGLAAMDISIWQEDTRCLSWLDGKPAGSVVYVNFGSMAVMTAAQAREFALGLASCGSPFLWVKRPDVVEGEEVLLPEALLDEVARGRGLVVPWCPQAAVLKHAAVGLFVSHCGWNSLLEATAAGQPVLAWPCHGEQTTNCRQLCEVWGNGAQLPREVESGAVARLVREMMVGDLGKEKRAKAAEWKAAAEAAARKGGASWRNVERVVNDLLLVGGKQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF199453
EMBL· GenBank· DDBJ
AAF17077.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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