Q9SB48 · NCPR1_ARATH

Function

function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and dichloroindophenol.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD per monomer.
FMN (UniProtKB | Rhea| CHEBI:58210 )

Note: Binds 1 FMN per monomer.

Biotechnology

Saccharomyces cerevisiae expressing Glycyrrhiza uralensis CYP88D6 and CYP72A154, combined with the expression of Arabidopsis thaliana beta-amyrin synthase (beta-AS) and NADPH-cytochrome P450 reductase 1 (ATR1), accumulates glycyrrhetinic acid (GA) and, to lower levels, beta-amyrin; these GA production was increased in the presence of G.uralensis cytochrome b5 (CYB5).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
2.2 μMNADPH7.025
21.9 μMNADPH7.728
2 μMNADPH7.2525
17 μMcytochrome c7.025
24.3 μMcytochrome c7.728

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site91-96FMN (UniProtKB | ChEBI)
Binding site146-149FMN (UniProtKB | ChEBI)
Binding site184-193FMN (UniProtKB | ChEBI)
Binding site219FMN (UniProtKB | ChEBI)
Binding site310NADP+ (UniProtKB | ChEBI)
Binding site470-473FAD (UniProtKB | ChEBI)
Binding site488-490FAD (UniProtKB | ChEBI)
Binding site504-507FAD (UniProtKB | ChEBI)
Binding site551NADP+ (UniProtKB | ChEBI)
Binding site612-613NADP+ (UniProtKB | ChEBI)
Binding site618-622NADP+ (UniProtKB | ChEBI)
Binding site654NADP+ (UniProtKB | ChEBI)
Binding site692FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentendoplasmic reticulum
Cellular Componentendoplasmic reticulum membrane
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctionNADP binding
Molecular FunctionNADPH-hemoprotein reductase activity
Biological Processphenylpropanoid metabolic process
Biological Processresponse to oxidative stress

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH--cytochrome P450 reductase 1
  • EC number
  • Short names
    AtCPR1
    ; CPR 1
    ; P450R 1
  • Alternative names
    • Cytochrome P450 73 A5

Gene names

    • Name
      ATR1
    • Synonyms
      AR1
      , CYP73A5
    • ORF names
      F22K18.280
    • Ordered locus names
      At4g24520

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Landsberg erecta
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9SB48
  • Secondary accessions
    • Q39035

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain2-26Lumenal
Transmembrane27-47Helical
Topological domain48-692Cytoplasmic

Keywords

Phenotypes & Variants

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 39 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylthreonine
ChainPRO_00004168392-692NADPH--cytochrome P450 reductase 1
Modified residue249Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in roots, leaves, stems, flowers and siliques.

Gene expression databases

Interaction

View interactors in UniProtKB
View CPX-2833 in Complex Portal

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain85-235Flavodoxin-like
Domain290-537FAD-binding FR-type

Sequence similarities

Belongs to the NADPH--cytochrome P450 reductase family.
In the N-terminal section; belongs to the flavodoxin family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q9SB48-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    692
  • Mass (Da)
    76,766
  • Last updated
    2000-05-01 v1
  • Checksum
    7DD77E418CCF2FA6
MTSALYASDLFKQLKSIMGTDSLSDDVVLVIATTSLALVAGFVVLLWKKTTADRSGELKPLMIPKSLMAKDEDDDLDLGSGKTRVSIFFGTQTGTAEGFAKALSEEIKARYEKAAVKVIDLDDYAADDDQYEEKLKKETLAFFCVATYGDGEPTDNAARFYKWFTEENERDIKLQQLAYGVFALGNRQYEHFNKIGIVLDEELCKKGAKRLIEVGLGDDDQSIEDDFNAWKESLWSELDKLLKDEDDKSVATPYTAVIPEYRVVTHDPRFTTQKSMESNVANGNTTIDIHHPCRVDVAVQKELHTHESDRSCIHLEFDISRTGITYETGDHVGVYAENHVEIVEEAGKLLGHSLDLVFSIHADKEDGSPLESAVPPPFPGPCTLGTGLARYADLLNPPRKSALVALAAYATEPSEAEKLKHLTSPDGKDEYSQWIVASQRSLLEVMAAFPSAKPPLGVFFAAIAPRLQPRYYSISSSPRLAPSRVHVTSALVYGPTPTGRIHKGVCSTWMKNAVPAEKSHECSGAPIFIRASNFKLPSNPSTPIVMVGPGTGLAPFRGFLQERMALKEDGEELGSSLLFFGCRNRQMDFIYEDELNNFVDQGVISELIMAFSREGAQKEYVQHKMMEKAAQVWDLIKEEGYLYVCGDAKGMARDVHRTLHTIVQEQEGVSSSEAEAIVKKLQTEGRYLRDVW

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
F4JQY4F4JQY4_ARATHATR1688

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict161in Ref. 1; CAA46814
Sequence conflict477-480in Ref. 1; CAA46814

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X66016
EMBL· GenBank· DDBJ
CAA46814.1
EMBL· GenBank· DDBJ
mRNA
AL035356
EMBL· GenBank· DDBJ
CAA23011.1
EMBL· GenBank· DDBJ
Genomic DNA
AL161561
EMBL· GenBank· DDBJ
CAB79362.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE84919.1
EMBL· GenBank· DDBJ
Genomic DNA
AY054688
EMBL· GenBank· DDBJ
AAK96879.1
EMBL· GenBank· DDBJ
mRNA
BT008426
EMBL· GenBank· DDBJ
AAP37785.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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