Q9S775 · PKL_ARATH
- ProteinCHD3-type chromatin-remodeling factor PICKLE
- GenePKL
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1384 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Chromatin remodeling factor that represses the expression of embryonic trait genes (such as NFYB9/LEC1) upon and after seed germination and thus enables the developmental switch to post-germinative growth. Silences some MADS-box proteins such as PHE1 and PHE2. Plays a role during carpel differentiation. Regulates late processes in cytokinin signaling.
Miscellaneous
'Gymnos' means 'naked' in Greek.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 298-305 | ATP (UniProtKB | ChEBI) | |||
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | helicase activity | |
Molecular Function | hydrolase activity | |
Molecular Function | metal ion binding | |
Biological Process | cell division | |
Biological Process | chromatin remodeling | |
Biological Process | cytokinin-activated signaling pathway | |
Biological Process | intracellular water homeostasis | |
Biological Process | negative regulation of abscisic acid-activated signaling pathway | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | regulation of lateral root development | |
Biological Process | response to auxin | |
Biological Process | response to gibberellin | |
Biological Process | response to water deprivation | |
Biological Process | root development |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCHD3-type chromatin-remodeling factor PICKLE
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9S775
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Hypersensitivity to cytokinins.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 301 | In chk2-2; hypersensitivity to cytokinins. | |||
Mutagenesis | 451 | In gym-4; when associated with a lack of CRC, morphological aberrations including shorter and narrower carpels containing external ovules. | |||
Mutagenesis | 633-635 | In pkl-1; embryonic traits expressed after germination including pickle roots (primary roots of adult plants that express embryonic differentiation traits such as expression of storage protein genes and accumulation of storage lipids). | |||
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000233174 | 1-1384 | CHD3-type chromatin-remodeling factor PICKLE | ||
Modified residue | 23 | Phosphoserine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Mostly expressed in tissue undergoing significant differentiation (meristems and primordia) such as young seedlings, influorescent tissue and young siliques, but not in endosperm and seed coat (at protein level). Levels decrease as organs age. Also present in trichomes.
Induction
Not up-regulated by cytokinins.
Gene expression databases
Structure
Family & Domains
Features
Showing features for zinc finger, domain, motif, compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Zinc finger | 49-96 | PHD-type | |||
Domain | 98-180 | Chromo 1 | |||
Domain | 190-249 | Chromo 2 | |||
Domain | 285-471 | Helicase ATP-binding | |||
Motif | 376-383 | Nuclear localization signal | |||
Motif | 422-425 | DEAH box | |||
Domain | 599-760 | Helicase C-terminal | |||
Compositional bias | 893-912 | Acidic residues | |||
Region | 893-941 | Disordered | |||
Region | 1122-1152 | Disordered | |||
Compositional bias | 1138-1152 | Polar residues | |||
Region | 1313-1344 | Disordered | |||
Compositional bias | 1316-1341 | Basic and acidic residues | |||
Region | 1365-1384 | Disordered | |||
Compositional bias | 1367-1384 | Basic and acidic residues | |||
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,384
- Mass (Da)158,405
- Last updated2000-05-01 v1
- MD5 Checksum19A15D1ED7B2A4D0EA75F092E51FD613
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8AZP2 | A0A1P8AZP2_ARATH | PKL | 1401 | ||
A0A1P8AZP6 | A0A1P8AZP6_ARATH | PKL | 1410 |
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 419 | in Ref. 5; BAF01271 | |||
Sequence conflict | 565 | in Ref. 5; BAF01271 | |||
Compositional bias | 893-912 | Acidic residues | |||
Compositional bias | 1138-1152 | Polar residues | |||
Compositional bias | 1316-1341 | Basic and acidic residues | |||
Compositional bias | 1367-1384 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF185578 EMBL· GenBank· DDBJ | AAF07084.1 EMBL· GenBank· DDBJ | mRNA | ||
AF185577 EMBL· GenBank· DDBJ | AAF13875.1 EMBL· GenBank· DDBJ | mRNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC07666.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | ANM62112.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK229409 EMBL· GenBank· DDBJ | BAF01271.1 EMBL· GenBank· DDBJ | mRNA |