Q9S702 · AK3_ARATH

Function

function

Involved in the first step of essential amino acids lysine, threonine, methionine and isoleucine synthesis via the aspartate-family pathway.

Catalytic activity

Activity regulation

Allosterically inhibited by lysine, but not by S-adenosyl-L-methionine (SAM). K(0.5) for lysine in the presence of physiological concentrations of substrates is 7.4 uM. No inhibition by threonine or leucine and no activation or inhibition by alanine, cysteine, isoleucine, serine, valine, methionine, glutamine, asparagine, glutamic acid or arginine.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
560 μMATP
1095 μMaspartate
K(cat) is 8.4/sec.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.
Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site88ATP (UniProtKB | ChEBI)
Binding site91ATP (UniProtKB | ChEBI)
Binding site120ATP (UniProtKB | ChEBI)
Binding site204substrate

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Functionaspartate kinase activity
Molecular FunctionATP binding
Biological Processlysine biosynthetic process via diaminopimelate
Biological Processphosphorylation
Biological Processthreonine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aspartokinase 3, chloroplastic
  • EC number
  • Alternative names
    • Aspartate kinase 3

Gene names

    • Name
      AK3
    • Synonyms
      AK-LYS3
    • ORF names
      F1C9.20, F28J7.35
    • Ordered locus names
      At3g02020

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9S702

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

Type
IDPosition(s)Description
Transit peptide1-85Chloroplast
ChainPRO_000024815986-559Aspartokinase 3, chloroplastic

Proteomic databases

Expression

Tissue specificity

Highly expressed in xylem of leaves and hypocotyls, stele of roots and in trichomes after bolting. Weak expression in veins and mesophyll cells of caulone leaves, inflorescence stems, sepals, petals and stigmata.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain402-480ACT 1
Domain481-559ACT 2

Sequence similarities

Belongs to the aspartokinase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    559
  • Mass (Da)
    61,216
  • Last updated
    2000-05-01 v1
  • Checksum
    FBC8A4A0E814F349
MAASMQFYGVKTPELALNSKRIEFSSKGLNFSALVSSARVFSRNVDRSCKNIALRVTCEAGRVELLERKASETFKLNKTEKKLTCVMKFGGSSVASAERMIQVAKLILSFPDEKPVVVLSAMAKTTNKLLMAGEKAVCCGVTNVDTIEELSYIKELHIRTAHELGVETAVIAEHLEGLEQLLKGVAMMKELTLRSRDYLVSFGECMSTRLFAAYLNKIGHKARQYDAFEIGIITTDDFTNADILEATYPAVSKKLLGDWSKENALPVVTGFLGKGWRSCAVTTLGRGGSDLTATTIGKALGLREIQVWKDVDGVLTCDPNIYCGAQPVPHLTFDEAAELAYFGAQVLHPLSMRPAREGNIPVRVKNSYNPTAPGTVITRSRDMSKAVLTSIVLKRNVTMLDITSTRMLGQYGFLAKVFSTFEKLGISVDVVATSEVSISLTLDPSKFCSRELIQHELDQVVEELEKIAVVNLLRHRSIISLIGNVQRSSFILEKGFRVLRTNGINVQMISQGASKVNISLIVNDDEAEHCVKALHSAFFETDTCEAVSECPTGYIAASS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC010797
EMBL· GenBank· DDBJ
AAF03452.1
EMBL· GenBank· DDBJ
Genomic DNA
AC011664
EMBL· GenBank· DDBJ
AAF14833.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE73750.1
EMBL· GenBank· DDBJ
Genomic DNA
AY088366
EMBL· GenBank· DDBJ
AAM65905.1
EMBL· GenBank· DDBJ
mRNA
AK226200
EMBL· GenBank· DDBJ
BAE98365.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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