Q9S702 · AK3_ARATH
- ProteinAspartokinase 3, chloroplastic
- GeneAK3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids559 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the first step of essential amino acids lysine, threonine, methionine and isoleucine synthesis via the aspartate-family pathway.
Catalytic activity
- L-aspartate + ATP = 4-phospho-L-aspartate + ADP
Activity regulation
Allosterically inhibited by lysine, but not by S-adenosyl-L-methionine (SAM). K(0.5) for lysine in the presence of physiological concentrations of substrates is 7.4 uM. No inhibition by threonine or leucine and no activation or inhibition by alanine, cysteine, isoleucine, serine, valine, methionine, glutamine, asparagine, glutamic acid or arginine.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
560 μM | ATP | |||||
1095 μM | aspartate |
K(cat) is 8.4/sec.
Pathway
Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.
Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 88 | ATP (UniProtKB | ChEBI) | |||
Binding site | 91 | ATP (UniProtKB | ChEBI) | |||
Binding site | 120 | ATP (UniProtKB | ChEBI) | |||
Binding site | 204 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | aspartate kinase activity | |
Molecular Function | ATP binding | |
Biological Process | lysine biosynthetic process via diaminopimelate | |
Biological Process | phosphorylation | |
Biological Process | threonine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAspartokinase 3, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9S702
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-85 | Chloroplast | |||
Chain | PRO_0000248159 | 86-559 | Aspartokinase 3, chloroplastic | ||
Proteomic databases
Expression
Tissue specificity
Highly expressed in xylem of leaves and hypocotyls, stele of roots and in trichomes after bolting. Weak expression in veins and mesophyll cells of caulone leaves, inflorescence stems, sepals, petals and stigmata.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 402-480 | ACT 1 | |||
Domain | 481-559 | ACT 2 | |||
Sequence similarities
Belongs to the aspartokinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length559
- Mass (Da)61,216
- Last updated2000-05-01 v1
- ChecksumFBC8A4A0E814F349
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC010797 EMBL· GenBank· DDBJ | AAF03452.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC011664 EMBL· GenBank· DDBJ | AAF14833.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE73750.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY088366 EMBL· GenBank· DDBJ | AAM65905.1 EMBL· GenBank· DDBJ | mRNA | ||
AK226200 EMBL· GenBank· DDBJ | BAE98365.1 EMBL· GenBank· DDBJ | mRNA |