Q9S4K6 · TIBC_ECOH1
- ProteinAutotransporter heptosyltransferase TibC
- GenetibC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids406 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Glycosylates adhesin TibA (PubMed:11953358, PubMed:25310236).
Specifically adds anomer D-glycero-beta-D-manno-heptose (PubMed:25310236).
Can not use ADP-L-glycero-beta-D-manno-heptose as a sugar donnor (PubMed:25310236).
Specifically adds anomer D-glycero-beta-D-manno-heptose (PubMed:25310236).
Can not use ADP-L-glycero-beta-D-manno-heptose as a sugar donnor (PubMed:25310236).
Miscellaneous
Able to functionally replace the autotransporter adhesin heptosyltransferase (AAH) of the AIDA system in fully restoring activity to the AIDA-I adhesin depending on the modification of AIDA-I with heptose residues.
Catalytic activity
- ADP-D-glycero-beta-D-manno-heptose + L-seryl-[protein] = ADP + H+ + O-(D-glycero-alpha-D-manno-heptosyl)-L-seryl-[protein]
Cofactor
Note: Binds 1 Fe3+ cation per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 107 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 108 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 109 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Active site | 110 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 224 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 226 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 230 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 257 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 281 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 302 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 326 | ADP-D-glycero-beta-D-manno-heptose (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 339 | Fe3+ (UniProtKB | ChEBI); structural | ||||
Sequence: C | ||||||
Binding site | 342 | Fe3+ (UniProtKB | ChEBI); structural | ||||
Sequence: C | ||||||
Binding site | 358 | Fe3+ (UniProtKB | ChEBI); structural | ||||
Sequence: C | ||||||
Binding site | 370 | Fe3+ (UniProtKB | ChEBI); structural | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | glycosyltransferase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAutotransporter heptosyltransferase TibC
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ9S4K6
- Secondary accessions
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 2-95 | Loss of catalytic activity. | ||||
Sequence: Missing | ||||||
Mutagenesis | 90 | No effect. | ||||
Sequence: E → K | ||||||
Mutagenesis | 110 | Loss of catalytic activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 230 | Loss of catalytic activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 265 | Loss of catalytic activity. Does not form homododecamer but forms homodimer. | ||||
Sequence: F → D | ||||||
Mutagenesis | 266 | Loss of catalytic activity. Does not form homododecamer but forms homodimer. | ||||
Sequence: V → D | ||||||
Mutagenesis | 286 | Severe loss of catalytic activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 300 | Loss of ligand stereospecificity. Can use both ADP-D,D-heptose and ADP-L,D-heptose as sugar donor. | ||||
Sequence: P → S | ||||||
Mutagenesis | 305 | Severe loss of catalytic activity. | ||||
Sequence: W → A | ||||||
Mutagenesis | 339 | Loss of catalytic activity. Loss of iron binding. Does not form homododecamer. | ||||
Sequence: C → S | ||||||
Mutagenesis | 342 | Loss of catalytic activity. Loss of iron binding. Does not form homododecamer. | ||||
Sequence: C → S | ||||||
Mutagenesis | 348 | No effect. | ||||
Sequence: L → P | ||||||
Mutagenesis | 358 | Loss of glycosyltransferase activity. | ||||
Sequence: C → R | ||||||
Mutagenesis | 358 | Loss of catalytic activity. Loss of iron binding. Does not form homododecamer. | ||||
Sequence: C → S | ||||||
Mutagenesis | 370 | Loss of catalytic activity. Loss of iron binding. Does not form homododecamer. | ||||
Sequence: C → S |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000076354 | 1-406 | Autotransporter heptosyltransferase TibC | |||
Sequence: MSTLKNTFFITPPDTPTQAGPENIFYDFNDGARVLLPEGKWHVRLLDADSENILFCCDVDKGWVTSSKKYFVRFRIQVFRQGEETPLLDETLKLKDRPVLISFPTGTLGDLLGWFPYAERFQSLHKCRLECTMSQDIIDLLAPQYPQIQFSTPDKPRTVAPYATYRVGLYFGGDTNNQPVDFRKVGFHRSAGYILGVDPREAPVRLDLSAPRVIQEPYVCIATQSTCQAKYWNNGTGWSEVIAHLKSLGYRVMCIDRDAHYGQGFVWNHIPWGAEDFTGKLPLQERVNLLRHASFFIGLPSGLSWLAWATRIPVVLISGFSLPNSEFYTPWRVFNSHGCYGCWDDTSLNFDHHDFLWCPRHKNTDRQFECTRLITGAQVNGVINKLHRSLTEQGVEATLKKGVSNE |
Interaction
Subunit
Homododecamer composed of 6 homodimers forming a ring.
Structure
Sequence
- Sequence statusComplete
- Length406
- Mass (Da)46,325
- Last updated2000-05-01 v1
- Checksum3FDDEA5C9D2FBDD5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF131891 EMBL· GenBank· DDBJ | AAD46996.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FN649414 EMBL· GenBank· DDBJ | CBJ01642.1 EMBL· GenBank· DDBJ | Genomic DNA |