Q9RFS6 · CYSH_BURCE
- ProteinAdenosine 5'-phosphosulfate reductase
- GenecysH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids250 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.
Catalytic activity
- [thioredoxin]-disulfide + AMP + 2 H+ + sulfite = [thioredoxin]-dithiol + adenosine 5'-phosphosulfate
RHEA-COMP:10700 CHEBI:50058 Position: n/n+3+ CHEBI:456215 + 2 CHEBI:15378 + CHEBI:17359 = RHEA-COMP:10698 CHEBI:29950 Position: nCHEBI:29950 Position: n+3+ CHEBI:58243
Cofactor
Note: Binds 1 [4Fe-4S] cluster per subunit.
Pathway
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 119 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 120 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 202 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 205 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Active site | 230 | Nucleophile; cysteine thiosulfonate intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | adenylyl-sulfate reductase (thioredoxin) activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoadenylyl-sulfate reductase (thioredoxin) activity | |
Biological Process | cysteine biosynthetic process | |
Biological Process | sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenosine 5'-phosphosulfate reductase
- EC number
- Short namesAPS reductase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Burkholderia > Burkholderia cepacia complex
Accessions
- Primary accessionQ9RFS6
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000424294 | 1-250 | Adenosine 5'-phosphosulfate reductase | |||
Sequence: MSTATATALTPELAAKVERLDALLAQIGERHDKVKFASSLAAEDMLLTHAILSKGVPIGIFSLNTGRLHAETLGMIDRVRERYGYEIEQFHPQQDAVDRYVAEHGLNAFYESVELRKSCCHIRKVEPLNRALADVGAWVTGQRREQSVTRAELHEEEQDEARGIAKYNPLADWTEADVWAYLKAFDVPVNPLHARGYPSIGCEPCTRAIRPGEDSRAGRWWWESRDTKECGLHITTITPIPANAEAGAAH |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length250
- Mass (Da)27,881
- Last updated2000-05-01 v1
- Checksum9E0E7F8001A4A5D9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF170343 EMBL· GenBank· DDBJ | AAD50979.1 EMBL· GenBank· DDBJ | Genomic DNA |