Q9R381 · AAC6_SALEN
- ProteinAminoglycoside N(6')-acetyltransferase type 1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids145 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the transfer of an acetyl group from acetyl-CoA to the 6'-amino group of aminoglycoside molecules conferring resistance to antibiotics containing the purpurosamine ring including amikacin, tobramycin, dibekacin and ribostamycin. Able to acetylate eukaryotic histone proteins.
Catalytic activity
- acetyl-CoA + kanamycin B = CoA + H+ + N(6')-acetylkanamycin B
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 22 | substrate | ||||
Sequence: W | ||||||
Binding site | 25 | substrate | ||||
Sequence: H | ||||||
Binding site | 66 | substrate | ||||
Sequence: Y | ||||||
Binding site | 79 | substrate | ||||
Sequence: E | ||||||
Binding site | 81-83 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: IFV | ||||||
Binding site | 89-94 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: QRGVAK | ||||||
Binding site | 115 | substrate | ||||
Sequence: D | ||||||
Binding site | 120 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 136 | substrate | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | aminoglycoside 6'-N-acetyltransferase activity | |
Molecular Function | protein homodimerization activity | |
Biological Process | acetyl-CoA metabolic process | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAminoglycoside N(6')-acetyltransferase type 1
- EC number
- Alternative names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionQ9R381
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000416829 | 1-145 | Aminoglycoside N(6')-acetyltransferase type 1 | |||
Sequence: MDIRQMNKTHLEHWRGLRKQLWPGHPDDAHLADGEEILQADHLASFIAMADGVAIGFADASIRHDYVNGCDSSPVVFLEGIFVLPSFRQRGVAKQLIAAVQRWGTNKGCREMASDTSPENTISQKVHQALGFEETERVIFYRKRC |
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-145 | N-acetyltransferase | ||||
Sequence: MDIRQMNKTHLEHWRGLRKQLWPGHPDDAHLADGEEILQADHLASFIAMADGVAIGFADASIRHDYVNGCDSSPVVFLEGIFVLPSFRQRGVAKQLIAAVQRWGTNKGCREMASDTSPENTISQKVHQALGFEETERVIFYRKRC |
Family and domain databases
Sequence
- Sequence statusComplete
- Length145
- Mass (Da)16,362
- Last updated2000-05-01 v1
- Checksum9CD8BBFD48BC43A2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF144880 EMBL· GenBank· DDBJ | AAF03531.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF144881 EMBL· GenBank· DDBJ | AAF03532.1 EMBL· GenBank· DDBJ | Genomic DNA |