Q9R016 · BIR1E_MOUSE

  • Protein
    Baculoviral IAP repeat-containing protein 1e
  • Gene
    Naip5
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Sensor component of the NLRC4 inflammasome that specifically recognizes and binds flagellin from pathogenic bacteria such as Legionella or Salmonella (PubMed:12526741, PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158).
Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158).
The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri (PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158).
May contribute to prevent motor-neuron apoptosis induced by a variety of signals (By similarity).

Features

Showing features for binding site.

114031002003004005006007008009001,0001,1001,2001,3001,400
TypeIDPosition(s)Description
Binding site315Zn2+ (UniProtKB | ChEBI)
Binding site318Zn2+ (UniProtKB | ChEBI)
Binding site335Zn2+ (UniProtKB | ChEBI)
Binding site342Zn2+ (UniProtKB | ChEBI)
Binding site473-478ATP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentIPAF inflammasome complex
Cellular Componentneuronal cell body
Cellular Componentperikaryon
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase inhibitor activity involved in apoptotic process
Molecular Functionmetal ion binding
Biological Processapoptotic process
Biological Processdefense response to bacterium
Biological Processdefense response to Gram-negative bacterium
Biological Processdetection of bacterium
Biological Processinflammatory response
Biological Processinnate immune response
Biological Processnegative regulation of apoptotic process
Biological Processpositive regulation of interleukin-1 beta production
Biological Processpyroptotic inflammatory response
Biological Processsymbiont entry into host cell

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Baculoviral IAP repeat-containing protein 1e
  • Alternative names
    • Neuronal apoptosis inhibitory protein 5

Gene names

    • Name
      Naip5
    • Synonyms
      Birc1e, Naip-rs3

Organism names

  • Taxonomic identifier
  • Strains
    • 129/Sv
    • P/J
    • C57BL/6J
    • 129/SvJ
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q9R016
  • Secondary accessions
    • O09121
    • O09122
    • P81703
    • Q8CGT2
    • Q9R029

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis1-40Abolishes production of IL1B in response to bacterial flagellin.
Mutagenesis11Strongly reduces production of IL1B in response to bacterial flagellin; then associated with F-15.
Mutagenesis15Strongly reduces production of IL1B in response to bacterial flagellin; then associated with I-11.
Mutagenesis106-108Strongly reduces production of IL1B in response to bacterial flagellin.
Mutagenesis626-627Strongly reduced interaction with flagellin.
Mutagenesis837-838Mildly reduced interaction with flagellin.
Mutagenesis839-840Mildly reduced interaction with flagellin.
Mutagenesis840Strongly reduces production of IL1B in response to bacterial flagellin.
Mutagenesis841-842Mildly reduced interaction with flagellin.
Mutagenesis843-844Strongly reduced interaction with flagellin.
Mutagenesis844Strongly reduces production of IL1B in response to bacterial flagellin.
Mutagenesis847Strongly reduces production of IL1B in response to bacterial flagellin.
Mutagenesis847Nearly abolishes interaction with flagellin.
Mutagenesis848-849Strongly reduced interaction with flagellin.
Mutagenesis857No effect on production of IL1B in response to bacterial flagellin.
Mutagenesis974-975Strongly reduced interaction with flagellin.
Mutagenesis1329-1330Reduced interaction with flagellin.
Mutagenesis1390-1403Abolishes production of IL1B in response to bacterial flagellin.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 153 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001223441-1403Baculoviral IAP repeat-containing protein 1e

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in macrophages (at protein level).

Gene expression databases

Interaction

Subunit

Component of the NLRC4 inflammasome, at least composed of NLRC4, caspase-1 (CASP1) and some NAIP protein. Flagellin binding by NAIP5 triggers assembly of the inflammasome, a huge complex that contains a single NAIP5 chain and multiple copies of NLRC4 (PubMed:29182158).
(Microbial infection) Interacts with S.typhimurium (Salmonella) flagellin.
(Microbial infection) Interacts with L.pneumophila flagellin.

Binary interactions

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for repeat, domain.

TypeIDPosition(s)Description
Repeat60-127BIR 1
Repeat159-227BIR 2
Repeat278-345BIR 3
Domain464-759NACHT

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,403
  • Mass (Da)
    159,839
  • Last updated
    2018-04-25 v3
  • Checksum
    04C04877908103EE
MAEHGESSEDRISEIDYEFLPELSALLGVDAFQVAKSQEEEEHKERMKMKKGFNSQMRSEAKRLKTFETYDTFRSWTPQEMAAAGFYHTGVRLGVQCFCCSLILFGNSLRKLPIERHKKLRPECEFLQGKDVGNIGKYDIRVKRPEKMLRGGKARYHEEEARLESFEDWPFYAHGTSPRVLSAAGFVFTGKRDTVQCFSCGGSLGNWEEGDDPWKEHAKWFPKCEFLQSKKSSEEIAQYIQSYEGFVHVTGEHFVKSWVRRELPMVSAYCNDSVFANEELRMDMFKDWPQESPVGVEALVRAGFFYTGKKDIVRCFSCGGCLEKWAEGDDPMEDHIKFFPECVFLQTLKSSAEVIPTLQSQYALPEATETTRESNHGDAAAVHSTVVDLGRSEAQWFQEARSLSEQLRDNYTKATFRHMNLPEVCSSLGTDHLLSCDVSIISKHISQPVQEALTIPEVFSNLNSVMCVEGETGSGKTTFLKRIAFLWASGCCPLLYRFQLVFYLSLSSITPDQGLANIICAQLLGAGGCISEVCLSSSIQQLQHQVLFLLDDYSGLASLPQALHTLITKNYLSRTCLLIAVHTNRVRDIRLYLGTSLEIQEFPFYNTVSVLRKFFSHDIICVEKLIIYFIDNKDLQGVYKTPLFVAAVCTDWIQNASAQDKFQDVTLFQSYMQYLSLKYKATAEPLQATVSSCGQLALTGLFSSCFEFNSDDLAEAGVDEDEKLTTLLMSKFTAQRLRPVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQIDSPLKAINSFNIFLYYVSSHSSSKAAPTVVSHLLQLVDEKESLENMSENEDYMKLHPQTFLWFQFVRGLWLVSPESSSSFVSEHLLRLALIFAYESNTVAECSPFILQFLRGKTLALRVLNLQYFRDHPESLLLLRSLKVSINGNKMSSYVDYSFKTYFENLQPPAIDEEYTSAFEHISEWRRNFAQDEEIIKNYENIRPRALPDISEGYWKLSPKPCKIPKLEVQVNNTDAADQALLQVLMEVFSASQSIEFRLFNSSGFLESICPALELSKASVTKCSMSRLELSRAEQELLLTLPALQSLEVSETNQLPEQLFHNLHKFLGLKELCVRLDGKPNVLSVLPREFPNLLHMEKLSIQTSTESDLSKLVKFIQNFPNLHVFHLKCDFLSNCESLMAVLASCKKLREIEFSGRCFEAMTFVNILPNFVSLKILNLKDQQFPDKETSEKFAQALGSLRNLEELLVPTGDGIHQVAKLIVRQCLQLPCLRVLTFHDILDDDSVIEIARAATSGGFQKLENLDISMNHKITEEGYRNFFQALDNLPNLQELNICRNIPGRIQVQATTVKALGQCVSRLPSLIRLHMLSWLLDEEDMKVINDVKERHPQSKRLIIFWKLIVPFSPVILE

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict92in Ref. 2; AAD56760 and 6; AAC52974
Sequence conflict144in Ref. 2; AAD56760 and 6; AAC52974
Sequence conflict242in Ref. 2; AAD56760
Sequence conflict472in Ref. 2; AAD56760
Sequence conflict516in Ref. 2; AAD56760
Sequence conflict521in Ref. 2; AAD56760
Sequence conflict533in Ref. 2; AAD56760
Sequence conflict538in Ref. 2; AAD56760
Sequence conflict1092in Ref. 2; AAD56760
Sequence conflict1116in Ref. 1; AAD56764 and 2; AAD56760
Sequence conflict1123in Ref. 1; AAD56764 and 2; AAD56760
Sequence conflict1129in Ref. 1; AAD56764
Sequence conflict1137in Ref. 1; AAD56764
Sequence conflict1242in Ref. 2; AAD56760
Sequence conflict1276in Ref. 2; AAD56760

Polymorphism

Part of the Lgn1 locus that determines susceptibility to the intracellular pathogen L.pneumophila. Susceptibility differs between inbred mouse strains. Strain C57BL/6J is not permissive, i.e. L.pneumophila cannot multiply in C57BL/6J macrophages, contrary to the situation in mouse strain A/J. Strain FVB/NJ macrophages display intermediate permissiveness for intracellular proliferation of L.pneumophila.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF135492
EMBL· GenBank· DDBJ
AAD56764.1
EMBL· GenBank· DDBJ
mRNA
AF131205
EMBL· GenBank· DDBJ
AAD56760.1
EMBL· GenBank· DDBJ
Genomic DNA
AY146999
EMBL· GenBank· DDBJ
AAN60211.1
EMBL· GenBank· DDBJ
mRNA
CT009518
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CT030167
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC070433
EMBL· GenBank· DDBJ
AAH70433.1
EMBL· GenBank· DDBJ
mRNA
U66326
EMBL· GenBank· DDBJ
AAC52974.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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