Q9QYE0 · PLAG1_MOUSE

  • Protein
    Zinc finger protein PLAG1
  • Gene
    Plag1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Cooperates with CBFB-MYH11.

Miscellaneous

Mice overexpressing Plag1 develop normally with high Plag1 transgene expression in salivary glands, spleen and weak mammary gland detection. They develop salivary gland tumors with major pathological features of human pleimorphic adenomas at the age of 1-5 months.
Mice with Plag1 overexpression targeted to salivary and mammary gland develop multifocal salivary gland tumors with pathological features of human pleimorphic adenomas at the age of 5 weeks, as well as mammary gland tumors at the age of 1 year. Plag1 overexpression in salivary gland are accompanied by increased IGFII expression. Besides features characteristic of benign pleimorphic adenomas, malignant characteristics are also observed in salivary gland tumors as well as metastasis to the lungs, reinforcing the tumorigenic role of Plag1.
Neonate mice carrying a human myeloid leukemia translocation-associated fusion gene CBFB-MYH11, injected with a retrovirus (4070A), develop the pathology within 2-5 months due to few viral insertions in some genes, such as PLAG1. Insertions near the transcription start site of PLAG1 are predominant in the related tumors and probably participate in the transformation process. Plag1 is one candidate gene that can cooperate with CBFB-MYH11 for leukemogenesis in this mouse model.

GO annotations

AspectTerm
Cellular Componentcentrosome
Cellular Componentcytosol
Cellular Componentnuclear speck
Molecular FunctionDNA-binding transcription activator activity, RNA polymerase II-specific
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Functionmetal ion binding
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Biological Processgland morphogenesis
Biological Processmulticellular organism growth
Biological Processnegative regulation of gene expression
Biological Processorgan growth
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of glial cell proliferation
Biological Processprostate gland growth
Biological Processregulation of DNA-templated transcription

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Zinc finger protein PLAG1
  • Alternative names
    • Pleiomorphic adenoma gene 1 protein

Gene names

    • Name
      Plag1

Organism names

  • Taxonomic identifier
  • Strains
    • 129/SvEv
    • 129/Sv
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q9QYE0
  • Secondary accessions
    • B1AXC4
    • Q3UXC0

Proteomes

Organism-specific databases

Subcellular Location

Note: Strong nucleolar localization when sumoylation is inhibited.

Keywords

Phenotypes & Variants

Disruption phenotype

Mice exhibit growth retardation with lower birth weight and disproportionally small seminal vesicles and ventral prostate as well as decreased fertility in both male and female. However, IGFII expression is normal in embryos.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Keywords

PTM/Processing

Features

Showing features for chain, cross-link.

TypeIDPosition(s)Description
ChainPRO_00002951081-499Zinc finger protein PLAG1
Cross-link244Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-link263Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modification

Sumoylated with SUMO1; which inhibits transcriptional activity, but does not affect nuclear localization. Blockers of sumoylation pathway such as SENP3 and inactive UBE2I increases transcriptional capacity. Sumoylation is increased in the presence of PIAS1 (By similarity).
Acetylated by lysine acetyltransferase EP300; which activates transcriptional capacity. Lysine residues that are sumoylated also seem to be target for acetylation (By similarity).

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in heart, spleen, lung, kidney, brain, testis and epididymis but not in salivary glands.

Developmental stage

High fetal expression with a strong decline after birth. Expressed at 9.5 dpc and 10.5 dpc in nervous system with highest level in telencephalon, diencephalon, and midbrain, as well as regionalized expression in the neural tube, which is characteristic of genes involved in the specification of neuronal fates.

Gene expression databases

Interaction

Subunit

Interacts with KPNA2, which escorts protein to the nucleus via interaction with nuclear localization signal. Interacts with E3 SUMO-protein ligase PIAS1, PIAS2 and PIAS4 (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, motif, zinc finger, compositional bias.

TypeIDPosition(s)Description
Region1-33Disordered
Region2-84Interaction with KPNA2
Motif22-25Nuclear localization signal
Zinc finger34-56C2H2-type 1
Region41-242Decreased nuclear import with localization in the nucleus but also in the cytoplasm
Zinc finger62-86C2H2-type 2
Zinc finger92-114C2H2-type 3
Zinc finger121-143C2H2-type 4
Zinc finger150-172C2H2-type 5
Zinc finger185-207C2H2-type 6
Zinc finger213-236C2H2-type 7
Region243-383Repression domain; contains 3 sumoylation motifs and massively decrease transcription activity
Region243-499Activates transcription; Inhibition of nuclear import due to lack of NLS and KPNA2 interaction
Compositional bias365-385Polar residues
Region365-400Disordered
Region384-499Massively activates transcription

Domain

C2H2-type zinc fingers 3 interacts with DNA-binding site G-clusterinc fingers. C2H2-type zinc fingers 6 and 7 interact with DNA-binding site core sequence (By similarity).

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    499
  • Mass (Da)
    55,579
  • Last updated
    2011-07-27 v2
  • Checksum
    A3493D2DADCB83B2
MATVIPGDLSEVRDTQKAPSGKRKRGESKPRKNFPCQLCDKAFNSVEKLKVHSFSHTGERPYKCTHQDCTKAFVSKYKLQRHMATHSPEKTHKCNYCEKMFHRKDHLKNHLHTHDPNKETFKCEECGKSYNTKLGFKRHLALHAATSGDLTCKVCLQNFESTGVLLEHLKSHAGKSSGGVKEKKHQCEHCERRFYTRKDVRRHMVVHTGRKDFLCQYCAQRFGRKDHLTRHMKKSHNQELLKVKTEPVDFLDPFTCNMSVPIKDELLPVMSLPSSELLSKPFTNTLQLNLYNTPFQSMQSSGSAHQMITTLPLGMTCPIDMDAVHPSHHLAFKCPFSSTSYAISIPEKEQPLKGEIESYLMELQGGAPSSSQDSPASSSKLGLEPQSGSPDDGAGDLSLSKSSISISDPLSTPALDFSQLFNFIPLNGPPYNPLSVGSLGMSYSQEEAHSSVSQLPTQTQDLQDPANTVGLSSLHSLSAAFTSSLSSSTTLPRFHQAFQ

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
D6RES7D6RES7_MOUSEPlag143

Sequence caution

The sequence BAE22643.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias365-385Polar residues
Sequence conflict433in Ref. 1; AAS86161 and 2; AAF21762

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY574219
EMBL· GenBank· DDBJ
AAS86161.1
EMBL· GenBank· DDBJ
mRNA
AF057366
EMBL· GenBank· DDBJ
AAF21762.1
EMBL· GenBank· DDBJ
mRNA
BC139283
EMBL· GenBank· DDBJ
AAI39284.1
EMBL· GenBank· DDBJ
mRNA
BC139285
EMBL· GenBank· DDBJ
AAI39286.1
EMBL· GenBank· DDBJ
mRNA
AL807387
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH466538
EMBL· GenBank· DDBJ
EDL05712.1
EMBL· GenBank· DDBJ
Genomic DNA
AK135759
EMBL· GenBank· DDBJ
BAE22643.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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