Q9QWH1 · PHC2_MOUSE
- ProteinPolyhomeotic-like protein 2
- GenePhc2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids850 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | heterochromatin | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Cellular Component | PRC1 complex | |
Molecular Function | chromatin binding | |
Molecular Function | DNA binding | |
Molecular Function | histone binding | |
Molecular Function | identical protein binding | |
Molecular Function | zinc ion binding | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | spermatogenesis |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePolyhomeotic-like protein 2
- Short namesmPH2
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9QWH1
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Co-localizes with SAMD7 in nuclear polycomb bodies.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mice are viable and fertile but show posterior transformations of the axial skeleton and premature senescence of mouse embryonic fibroblast associated with derepression of Hox cluster genes and Cdkn2a genes, respectively. Mice lacking Phc2 and Phc1 die at an early gestational stage. Mice mutant for Phc1 and/or Phc2 demonstrate that Phc1 and Phc2 mutations affect synergistically the survival of embryos in a gene dosage-dependent manner and thus show functional redundancy of Phc1 and Phc2.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 42 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, cross-link, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000076287 | 1-850 | Polyhomeotic-like protein 2 | |||
Sequence: MENELPVPHTSNRASVTTNTSGTNSSSGCISSSGGGGGSGGRPTAPQISVYSGIPDRQTVQVIQQALHRQPSTAAQYLQQMYAAQQQHLMLQTAALQQQHLSSAQLQSLAAVQQASLVANRQGSTPGSSVSSQAPAQSSSLNLAASPAAAQLINRAQSVNSAAASGLAQQAVLLGNTSSPALTASQAQMYLRAQMLIFTPTATVATVQPELCTGSPARPPTPAQVQNLTLRTQQTPAAAASGPPPTQPVLPSLALKPTPSSSQPLPAPPQGRTMAQGSPAGAKPSGTDNAPETLKAGDGNCNMEGRPGPGRAVPAVATHPLIAPAYAHLQSHQLLPQPPAKHPQPQFVAQQQPQPPRPAPQVQSQPQLASVSPSLALQSSPEDHALPLGSVTQALPLQCSTTHVHKPGNSQQCHLPTLDTGSQNGHPEGGSHPPQRRFQHTSAVILQVQPASPVTPQQCAPDDWKEVVPAEKSVPVARPGPSPHQQAIIPAIPGGLPGPKSPNIQQCPAHETGQGIVHALTDLSSPGMTSGNGNSASSIAGTAPQNGENKPPQAIVKPQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGFLPEKPPQQDHTTTTDSEMEEPYLQESKEEGTPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTKRVGLFHSDRSKLQKAGTTTHNRRRASKASLPTLTKDTKKQPSGTVPLSVTAALQLAHSQEDSSRCSDNSSYEEPLSPISASSSTSRRRQGQRDLDLPDMHMRDLVGVGHHFLPSEPTKWNVEDVYEFIRSLPGCQEIAEEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS | ||||||
Cross-link | 590 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 592 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 611 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 613 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 624 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 694 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 743 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 839 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Isoform 2 is ubiquitously expressed in embryos and adult tissues at much higher level than isoform 1.
Developmental stage
Detected at 11.5 dpc in the developing brain, in the ventricular zones of the cortex and ganglionic eminences as well as in adult, in mature structures such as the granule cell layer of the dentate gyrus and cerebellum.
Gene expression databases
Interaction
Subunit
Component of a PRC1-like complex. Interacts with CBX4 (By similarity).
Interacts with BMI1, PCGF2, PHC1 and RNF2 (PubMed:16024804, PubMed:27827373, PubMed:9627119).
Interacts with CHTOP (PubMed:22872859).
Interacts with the N-terminal region of the SP1 transcription factor and with MAPKAPK2 (By similarity).
Interacts with SAMD7 (PubMed:28900001).
Interacts with SAMD11 (PubMed:27827373).
Interacts with BMI1, PCGF2, PHC1 and RNF2 (PubMed:16024804, PubMed:27827373, PubMed:9627119).
Interacts with CHTOP (PubMed:22872859).
Interacts with the N-terminal region of the SP1 transcription factor and with MAPKAPK2 (By similarity).
Interacts with SAMD7 (PubMed:28900001).
Interacts with SAMD11 (PubMed:27827373).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9QWH1 | Bmi1 P25916 | 3 | EBI-642357, EBI-927401 | |
BINARY | Q9QWH1 | Mapkapk2 P49138 | 5 | EBI-642357, EBI-298776 | |
XENO | Q9QWH1 | MAPKAPK2 P49137 | 2 | EBI-642357, EBI-993299 | |
BINARY | Q9QWH1 | Pcgf2 P23798 | 5 | EBI-642357, EBI-926857 | |
BINARY | Q9QWH1 | Phc1 Q64028 | 2 | EBI-642357, EBI-927346 | |
BINARY | Q9QWH1 | Ring1 O35730 | 2 | EBI-642357, EBI-929310 | |
BINARY | Q9QWH1 | Rnf2 Q9CQJ4 | 7 | EBI-642357, EBI-927321 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region, motif, zinc finger, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-38 | Polar residues | ||||
Sequence: MENELPVPHTSNRASVTTNTSGTNSSSGCISSSGGGGG | ||||||
Region | 1-79 | Disordered | ||||
Sequence: MENELPVPHTSNRASVTTNTSGTNSSSGCISSSGGGGGSGGRPTAPQISVYSGIPDRQTVQVIQQALHRQPSTAAQYLQ | ||||||
Region | 33-56 | Interaction with BMI1 | ||||
Sequence: SGGGGGSGGRPTAPQISVYSGIPD | ||||||
Region | 233-314 | Disordered | ||||
Sequence: QQTPAAAASGPPPTQPVLPSLALKPTPSSSQPLPAPPQGRTMAQGSPAGAKPSGTDNAPETLKAGDGNCNMEGRPGPGRAVP | ||||||
Region | 335-386 | Disordered | ||||
Sequence: LPQPPAKHPQPQFVAQQQPQPPRPAPQVQSQPQLASVSPSLALQSSPEDHAL | ||||||
Compositional bias | 338-362 | Pro residues | ||||
Sequence: PPAKHPQPQFVAQQQPQPPRPAPQV | ||||||
Compositional bias | 363-379 | Polar residues | ||||
Sequence: QSQPQLASVSPSLALQS | ||||||
Region | 402-436 | Disordered | ||||
Sequence: THVHKPGNSQQCHLPTLDTGSQNGHPEGGSHPPQR | ||||||
Compositional bias | 528-549 | Polar residues | ||||
Sequence: MTSGNGNSASSIAGTAPQNGEN | ||||||
Region | 528-553 | Disordered | ||||
Sequence: MTSGNGNSASSIAGTAPQNGENKPPQ | ||||||
Motif | 550-579 | HD1 | ||||
Sequence: KPPQAIVKPQILTHVIEGFVIQEGAEPFPV | ||||||
Region | 597-624 | Disordered | ||||
Sequence: FLPEKPPQQDHTTTTDSEMEEPYLQESK | ||||||
Compositional bias | 610-624 | Basic and acidic residues | ||||
Sequence: TTDSEMEEPYLQESK | ||||||
Zinc finger | 625-659 | FCS-type | ||||
Sequence: EEGTPLKLKCELCGRVDFAYKFKRSKRFCSMACAK | ||||||
Region | 676-712 | Disordered | ||||
Sequence: RSKLQKAGTTTHNRRRASKASLPTLTKDTKKQPSGTV | ||||||
Compositional bias | 689-712 | Polar residues | ||||
Sequence: RRRASKASLPTLTKDTKKQPSGTV | ||||||
Compositional bias | 725-754 | Polar residues | ||||
Sequence: SQEDSSRCSDNSSYEEPLSPISASSSTSRR | ||||||
Region | 725-764 | Disordered | ||||
Sequence: SQEDSSRCSDNSSYEEPLSPISASSSTSRRRQGQRDLDLP | ||||||
Domain | 786-850 | SAM | ||||
Sequence: WNVEDVYEFIRSLPGCQEIAEEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS |
Domain
HD1 motif interacts with SAM domain of PHC1.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9QWH1-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length850
- Mass (Da)89,799
- Last updated2000-05-01 v1
- Checksum87746AFD7808DC7B
Q9QWH1-2
- Name2
- Differences from canonical
- 1-527: Missing
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-38 | Polar residues | ||||
Sequence: MENELPVPHTSNRASVTTNTSGTNSSSGCISSSGGGGG | ||||||
Alternative sequence | VSP_016919 | 1-527 | in isoform 2 | |||
Sequence: Missing | ||||||
Compositional bias | 338-362 | Pro residues | ||||
Sequence: PPAKHPQPQFVAQQQPQPPRPAPQV | ||||||
Compositional bias | 363-379 | Polar residues | ||||
Sequence: QSQPQLASVSPSLALQS | ||||||
Compositional bias | 528-549 | Polar residues | ||||
Sequence: MTSGNGNSASSIAGTAPQNGEN | ||||||
Compositional bias | 610-624 | Basic and acidic residues | ||||
Sequence: TTDSEMEEPYLQESK | ||||||
Compositional bias | 689-712 | Polar residues | ||||
Sequence: RRRASKASLPTLTKDTKKQPSGTV | ||||||
Compositional bias | 725-754 | Polar residues | ||||
Sequence: SQEDSSRCSDNSSYEEPLSPISASSSTSRR |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB062362 EMBL· GenBank· DDBJ | BAB93527.1 EMBL· GenBank· DDBJ | mRNA | ||
U81491 EMBL· GenBank· DDBJ | AAD00519.1 EMBL· GenBank· DDBJ | mRNA | ||
AL611969 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL611983 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC057571 EMBL· GenBank· DDBJ | AAH57571.1 EMBL· GenBank· DDBJ | mRNA | ||
BC071246 EMBL· GenBank· DDBJ | AAH71246.1 EMBL· GenBank· DDBJ | mRNA | ||
AF060076 EMBL· GenBank· DDBJ | AAC23570.1 EMBL· GenBank· DDBJ | mRNA |