Q9PZT1 · NS1_PAVHV

Function

function

Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promoter (PubMed:11853402, PubMed:24418564, PubMed:27809499).
Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR) (PubMed:27809499).
Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters (PubMed:11853402, PubMed:8970971).
Transactivates several host promoters some of which induce the S cell cycle phase for the production of host replicative proteins (PubMed:15518826, PubMed:20890043, PubMed:24049177).
Up-regulates the expression of host E2F4 and E2F5 and interacts with both these factors thereby inhibiting the host cell cycle G2/M transition (PubMed:20890043).
This arrest promotes apoptosis for viral release (PubMed:20890043, PubMed:9525624).

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: The endonuclease active site can probably bind other divalent cations.

pH Dependence

Optimum pH is 7.0-7.5.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site72a divalent metal cation (UniProtKB | ChEBI)
Binding site81a divalent metal cation (UniProtKB | ChEBI)
Binding site83a divalent metal cation (UniProtKB | ChEBI)
Active site141For nuclease activity
Binding site328-335ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA binding
Molecular Functionendonuclease activity
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Biological ProcessDNA replication
Biological Processrolling hairpin viral DNA replication
Biological Processsymbiont-mediated activation of host apoptosis
Biological Processsymbiont-mediated arrest of host cell cycle during G2/M transition
Biological Processsymbiont-mediated perturbation of host transcription

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Initiator protein NS1
  • EC number
  • Short names
    NS1
  • Alternative names
    • NCVP1
    • Non-capsid protein NS-1
    • Non-structural protein 1
    • Non-structural protein NS1

Gene names

    • Name
      NS1

Organism names

Accessions

  • Primary accession
    Q9PZT1

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis177Complete loss of nuclear localization. Complete loss of NS1-mediated nuclear translocation of host E2F4 and E2F5, although still up-regulating their expression.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004287151-671Initiator protein NS1

Keywords

Interaction

Subunit

Homooligomer; when bound to DNA (PubMed:27809499).
Interacts with host E2F4 and E2F5; these interactions promote the nuclear import of E2F4 and E2F5 and the G2/M block observed in host cells (PubMed:20890043).
Interacts with host transcription factor SP1; this interaction is important for NS1 transcriptional regulation of p21/WAF1 (PubMed:11853402, PubMed:15518826).
Interacts with host transcription factor SP3 (PubMed:11853402).

Structure

Family & Domains

Features

Showing features for region, domain, motif.

TypeIDPosition(s)Description
Region1-176Endonuclease
Domain1-186PV NS1-Nuc
Motif81-83RCR-2
Motif141-145RCR-3
Motif177-180Nuclear localization signal
Domain302-457SF3 helicase
Region532-553Disordered

Domain

In the N-terminus, the endonuclease region is involved in binding to the origin of replication on the viral DNA as well as to the overlapping viral promoter p6. In the middle, there are the ATPase and helicase activities. The C-terminus probably contains a transactivation domain.

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    671
  • Mass (Da)
    74,065
  • Last updated
    2000-05-01 v1
  • Checksum
    503EFCDC87A0BF74
MELFRGVLQVSSNVLDCANDNWWCSLLDLDTSDWEPLTHTNRLMAIYLSSVASKLDFTGGPLAGCLYFFQVECNKFEEGYHIHVVIGGPGLNPRNLTVCVEGLFNNVLYHLVTENVKLKFLPGMTTKGKYFRDGEQFIENYLMKKIPLNVVWCVTNIDGYIDTCISATFRRGACHAKKPRITTAINDTSSDAGESSGTGAEVVPINGKGTKASIKFQTMVNWLCENRVFTEDKWKLVDFNQYTLLSSSHSGSFQIQSALKLAIYKATNLVPTSTFLLHTDFEQVMCIKDNKIVKLLLCQNYDPLLVGQHVLKWIDKKCGKKNTLWFYGPPSTGKTNLAMAIAKSVPVYGMVNWNNENFPFNDVAGKSLVVWDEGIIKSTIVEAAKAILGGQPTRVDQKMRGSVAVPGVPVVITSNGDITFVVSGNTTTTVHAKALKERMVKLNFTVRCSPDMGLLTEADVQQWLTWCNAQSWDHYENWAINYTFDFPGINADALHPDLQTTPIVTDTSISSSGGESSEELSESSFFNLITPGAWNTETPRSSTPIPGTSSGESFVGSSVSSEVVAASWEEAFYTPLADQFRELLVGVDYVWDGVRGLPVCCVQHINNSGGGLGLCPHCINVGAWYNGWKFREFTPDLVRCSCHVGASNPFSVLTCKKCAYLSGLQSFVDYE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF162273
EMBL· GenBank· DDBJ
AAD46613.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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