Q9PZT1 · NS1_PAVHV
- ProteinInitiator protein NS1
- GeneNS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids671 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promoter (PubMed:11853402, PubMed:24418564, PubMed:27809499).
Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR) (PubMed:27809499).
Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters (PubMed:11853402, PubMed:8970971).
Transactivates several host promoters some of which induce the S cell cycle phase for the production of host replicative proteins (PubMed:15518826, PubMed:20890043, PubMed:24049177).
Up-regulates the expression of host E2F4 and E2F5 and interacts with both these factors thereby inhibiting the host cell cycle G2/M transition (PubMed:20890043).
This arrest promotes apoptosis for viral release (PubMed:20890043, PubMed:9525624).
Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR) (PubMed:27809499).
Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters (PubMed:11853402, PubMed:8970971).
Transactivates several host promoters some of which induce the S cell cycle phase for the production of host replicative proteins (PubMed:15518826, PubMed:20890043, PubMed:24049177).
Up-regulates the expression of host E2F4 and E2F5 and interacts with both these factors thereby inhibiting the host cell cycle G2/M transition (PubMed:20890043).
This arrest promotes apoptosis for viral release (PubMed:20890043, PubMed:9525624).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Note: The endonuclease active site can probably bind other divalent cations.
pH Dependence
Optimum pH is 7.0-7.5.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 72 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 81 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 83 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 141 | For nuclease activity | ||||
Sequence: Y | ||||||
Binding site | 328-335 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GPPSTGKT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | helicase activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA replication | |
Biological Process | rolling hairpin viral DNA replication | |
Biological Process | symbiont-mediated activation of host apoptosis | |
Biological Process | symbiont-mediated arrest of host cell cycle during G2/M transition | |
Biological Process | symbiont-mediated perturbation of host transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInitiator protein NS1
- EC number
- Short namesNS1
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Monodnaviria > Shotokuvirae > Cossaviricota > Quintoviricetes > Piccovirales > Parvoviridae > Parvovirinae > Erythroparvovirus > Erythroparvovirus primate1
- Virus hosts
Accessions
- Primary accessionQ9PZT1
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 177 | Complete loss of nuclear localization. Complete loss of NS1-mediated nuclear translocation of host E2F4 and E2F5, although still up-regulating their expression. | ||||
Sequence: K → C |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000428715 | 1-671 | Initiator protein NS1 | |||
Sequence: MELFRGVLQVSSNVLDCANDNWWCSLLDLDTSDWEPLTHTNRLMAIYLSSVASKLDFTGGPLAGCLYFFQVECNKFEEGYHIHVVIGGPGLNPRNLTVCVEGLFNNVLYHLVTENVKLKFLPGMTTKGKYFRDGEQFIENYLMKKIPLNVVWCVTNIDGYIDTCISATFRRGACHAKKPRITTAINDTSSDAGESSGTGAEVVPINGKGTKASIKFQTMVNWLCENRVFTEDKWKLVDFNQYTLLSSSHSGSFQIQSALKLAIYKATNLVPTSTFLLHTDFEQVMCIKDNKIVKLLLCQNYDPLLVGQHVLKWIDKKCGKKNTLWFYGPPSTGKTNLAMAIAKSVPVYGMVNWNNENFPFNDVAGKSLVVWDEGIIKSTIVEAAKAILGGQPTRVDQKMRGSVAVPGVPVVITSNGDITFVVSGNTTTTVHAKALKERMVKLNFTVRCSPDMGLLTEADVQQWLTWCNAQSWDHYENWAINYTFDFPGINADALHPDLQTTPIVTDTSISSSGGESSEELSESSFFNLITPGAWNTETPRSSTPIPGTSSGESFVGSSVSSEVVAASWEEAFYTPLADQFRELLVGVDYVWDGVRGLPVCCVQHINNSGGGLGLCPHCINVGAWYNGWKFREFTPDLVRCSCHVGASNPFSVLTCKKCAYLSGLQSFVDYE |
Keywords
- PTM
Interaction
Subunit
Homooligomer; when bound to DNA (PubMed:27809499).
Interacts with host E2F4 and E2F5; these interactions promote the nuclear import of E2F4 and E2F5 and the G2/M block observed in host cells (PubMed:20890043).
Interacts with host transcription factor SP1; this interaction is important for NS1 transcriptional regulation of p21/WAF1 (PubMed:11853402, PubMed:15518826).
Interacts with host transcription factor SP3 (PubMed:11853402).
Interacts with host E2F4 and E2F5; these interactions promote the nuclear import of E2F4 and E2F5 and the G2/M block observed in host cells (PubMed:20890043).
Interacts with host transcription factor SP1; this interaction is important for NS1 transcriptional regulation of p21/WAF1 (PubMed:11853402, PubMed:15518826).
Interacts with host transcription factor SP3 (PubMed:11853402).
Family & Domains
Features
Showing features for region, domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-176 | Endonuclease | ||||
Sequence: MELFRGVLQVSSNVLDCANDNWWCSLLDLDTSDWEPLTHTNRLMAIYLSSVASKLDFTGGPLAGCLYFFQVECNKFEEGYHIHVVIGGPGLNPRNLTVCVEGLFNNVLYHLVTENVKLKFLPGMTTKGKYFRDGEQFIENYLMKKIPLNVVWCVTNIDGYIDTCISATFRRGACHA | ||||||
Domain | 1-186 | PV NS1-Nuc | ||||
Sequence: MELFRGVLQVSSNVLDCANDNWWCSLLDLDTSDWEPLTHTNRLMAIYLSSVASKLDFTGGPLAGCLYFFQVECNKFEEGYHIHVVIGGPGLNPRNLTVCVEGLFNNVLYHLVTENVKLKFLPGMTTKGKYFRDGEQFIENYLMKKIPLNVVWCVTNIDGYIDTCISATFRRGACHAKKPRITTAIN | ||||||
Motif | 81-83 | RCR-2 | ||||
Sequence: HIH | ||||||
Motif | 141-145 | RCR-3 | ||||
Sequence: YLMKK | ||||||
Motif | 177-180 | Nuclear localization signal | ||||
Sequence: KKPR | ||||||
Domain | 302-457 | SF3 helicase | ||||
Sequence: DPLLVGQHVLKWIDKKCGKKNTLWFYGPPSTGKTNLAMAIAKSVPVYGMVNWNNENFPFNDVAGKSLVVWDEGIIKSTIVEAAKAILGGQPTRVDQKMRGSVAVPGVPVVITSNGDITFVVSGNTTTTVHAKALKERMVKLNFTVRCSPDMGLLTE | ||||||
Region | 532-553 | Disordered | ||||
Sequence: GAWNTETPRSSTPIPGTSSGES |
Domain
In the N-terminus, the endonuclease region is involved in binding to the origin of replication on the viral DNA as well as to the overlapping viral promoter p6. In the middle, there are the ATPase and helicase activities. The C-terminus probably contains a transactivation domain.
Sequence similarities
Belongs to the parvoviruses initiator protein NS1 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length671
- Mass (Da)74,065
- Last updated2000-05-01 v1
- Checksum503EFCDC87A0BF74
Keywords
- Technical term