Q9PM40 · RNC_CAMJE

Function

function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (By similarity).
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA (Probable). Complements pre-crRNA and tracrRNA coprocessing defects in an rnc deletion in S.pyogenes strain 370 (PubMed:24270795).

Catalytic activity

  • Endonucleolytic cleavage to 5'-phosphomonoester.
    EC:3.1.26.3 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site40Mg2+ (UniProtKB | ChEBI)
Active site44
Binding site113Mg2+ (UniProtKB | ChEBI)
Active site116
Binding site116Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionmetal ion binding
Molecular Functionribonuclease III activity
Molecular FunctionrRNA binding
Biological ProcessmRNA processing
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease 3
  • EC number
  • Alternative names
    • Ribonuclease III
      (RNase III
      )

Gene names

    • Name
      rnc
    • Ordered locus names
      Cj1635c

Organism names

Accessions

  • Primary accession
    Q9PM40
  • Secondary accessions
    • Q0P7Z3

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001803831-224Ribonuclease 3

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-127RNase III
Domain154-223DRBM

Sequence similarities

Belongs to the ribonuclease III family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    224
  • Mass (Da)
    24,964
  • Last updated
    2000-10-01 v1
  • Checksum
    4AB69FD394DB17CA
MKNIEKLEQSLTYEFKDKNLLIHALTHKSFKKSYNNERLEFLGDAVLDLVVGEYLFHKFAKDAEGDLSKLRAALVNEKSFAKIANSLNLGDFILMSVAEENNGGKEKPSILSDALEAIIGAIHLEAGFEFAKTIALRLIEKNFPQIDAKILIKDYKTKLQEITQGKIGQTPQYETVRAFGPDHLKQFEIALMLDGKELARAIAGSKKEAQQMAAKIALEKLGAL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL111168
EMBL· GenBank· DDBJ
CAL35732.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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