Q9PLT8 · RECB_CHLMU
- ProteinRecBCD enzyme subunit RecB
- GenerecB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1026 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.
Miscellaneous
In the RecBCD complex, RecB has a slow 3'-5' helicase, an exonuclease activity and loads RecA onto ssDNA, RecD has a fast 5'-3' helicase activity, while RecC stimulates the ATPase and processivity of the RecB helicase and contributes to recognition of the Chi site.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 3'-5' DNA helicase activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | exodeoxyribonuclease V activity | |
Molecular Function | isomerase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | double-strand break repair via homologous recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRecBCD enzyme subunit RecB
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Chlamydiota > Chlamydiia > Chlamydiales > Chlamydiaceae > Chlamydia/Chlamydophila group > Chlamydia
Accessions
- Primary accessionQ9PLT8
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000102043 | 1-1026 | RecBCD enzyme subunit RecB | |||
Sequence: MSSFDIFSPTTSVSGKFFLEASAGTGKTFTIEQVILRSLLEGNVEQTKNILVVTFTNAATNELKLRIQESLKQALTLFSQALSHPETPLPPYVSSQETKVKQLYXKXRNSLATLDEMNIFTIHGLCRFTLEQHFPWVQPIHPSSMFSEPQTIQQYILDYLRQNSWENVLSPKQYAFLSYHHRATTQQTRHLADRLLQDYASTPNLALPPLSITLQKVKNWSSQYKHLSPLSLEELQDFSLRFKQSDLPIDRELPDFVKQFETDPNSLDILFFPGMVQKFQEENRNKKKLGPPFSPLDPFLKDWLLIAQPFCQKEPIFHTLLKSVQQHLKTYCAQSYSHDESIATLESLLAQNDHVVSQLRKQFQLVLIDEFQDTDKRQWKIFSKLFASPDYSGSLFLIGDPKQSIYEWRNADLPTYLQAKNSFPKESQLILDTNYRSTPQLMQALNHLFSLPSPFLETPQTILYHPLQSKGSASTSYSEFRPIHFFTTQDSQEEALWISKTASYLRSTFAIPFGNMAVLVQDYPQALKLITHSTIPMAYCKEKRIFDRTESPYLLILLLEALLYPENQQKIQAILLSRFFHLSATDIHQHLKIFSSLFFMLNTYLHQYSLLATFYKLMGETVFSQTIGETLLQTPLGDIIFQELEELCLYLDKTTENPHHKLFHLINILDTGKYDEELSFSSQSNDDNVLKITTVHSSKGLEYDVVFCSSLNKVKEKSPSVHMREMYVACTRAKKFLFIPFSSIEKRLQSNKKVSALANYANITQHDNIPHLVETLTASHPEFFSSGTQPPESNISIFSEPLPEQEFFSLPILSSQPIYSFSSTTESQYFTEPFQEISSSSLFPGGSLTGTLIHKLLESLSGNFNASLEEITHKAQTLLKNTILEGFESIISEKIYTAFSTKLPFVSGSFALKDVHPYNIRVEETFLLQENGELWQGIVDLFFEHNNRFFIIDWKTSFLGDEPSLYSPDKLLLYIQRQGLDKQGVLYKRAAKKFLHQFNSSLQIEMAFVFIRGIDDKGNGFLQPSP |
Interaction
Subunit
Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-438 | UvrD-like helicase ATP-binding | ||||
Sequence: MSSFDIFSPTTSVSGKFFLEASAGTGKTFTIEQVILRSLLEGNVEQTKNILVVTFTNAATNELKLRIQESLKQALTLFSQALSHPETPLPPYVSSQETKVKQLYXKXRNSLATLDEMNIFTIHGLCRFTLEQHFPWVQPIHPSSMFSEPQTIQQYILDYLRQNSWENVLSPKQYAFLSYHHRATTQQTRHLADRLLQDYASTPNLALPPLSITLQKVKNWSSQYKHLSPLSLEELQDFSLRFKQSDLPIDRELPDFVKQFETDPNSLDILFFPGMVQKFQEENRNKKKLGPPFSPLDPFLKDWLLIAQPFCQKEPIFHTLLKSVQQHLKTYCAQSYSHDESIATLESLLAQNDHVVSQLRKQFQLVLIDEFQDTDKRQWKIFSKLFASPDYSGSLFLIGDPKQSIYEWRNADLPTYLQAKNSFPKESQLILDTNYRST | ||||||
Region | 1-766 | DNA-binding and helicase activity, interacts with RecC | ||||
Sequence: MSSFDIFSPTTSVSGKFFLEASAGTGKTFTIEQVILRSLLEGNVEQTKNILVVTFTNAATNELKLRIQESLKQALTLFSQALSHPETPLPPYVSSQETKVKQLYXKXRNSLATLDEMNIFTIHGLCRFTLEQHFPWVQPIHPSSMFSEPQTIQQYILDYLRQNSWENVLSPKQYAFLSYHHRATTQQTRHLADRLLQDYASTPNLALPPLSITLQKVKNWSSQYKHLSPLSLEELQDFSLRFKQSDLPIDRELPDFVKQFETDPNSLDILFFPGMVQKFQEENRNKKKLGPPFSPLDPFLKDWLLIAQPFCQKEPIFHTLLKSVQQHLKTYCAQSYSHDESIATLESLLAQNDHVVSQLRKQFQLVLIDEFQDTDKRQWKIFSKLFASPDYSGSLFLIGDPKQSIYEWRNADLPTYLQAKNSFPKESQLILDTNYRSTPQLMQALNHLFSLPSPFLETPQTILYHPLQSKGSASTSYSEFRPIHFFTTQDSQEEALWISKTASYLRSTFAIPFGNMAVLVQDYPQALKLITHSTIPMAYCKEKRIFDRTESPYLLILLLEALLYPENQQKIQAILLSRFFHLSATDIHQHLKIFSSLFFMLNTYLHQYSLLATFYKLMGETVFSQTIGETLLQTPLGDIIFQELEELCLYLDKTTENPHHKLFHLINILDTGKYDEELSFSSQSNDDNVLKITTVHSSKGLEYDVVFCSSLNKVKEKSPSVHMREMYVACTRAKKFLFIPFSSIEKRLQSNKKVSALANYANITQH | ||||||
Domain | 452-700 | UvrD-like helicase C-terminal | ||||
Sequence: PSPFLETPQTILYHPLQSKGSASTSYSEFRPIHFFTTQDSQEEALWISKTASYLRSTFAIPFGNMAVLVQDYPQALKLITHSTIPMAYCKEKRIFDRTESPYLLILLLEALLYPENQQKIQAILLSRFFHLSATDIHQHLKIFSSLFFMLNTYLHQYSLLATFYKLMGETVFSQTIGETLLQTPLGDIIFQELEELCLYLDKTTENPHHKLFHLINILDTGKYDEELSFSSQSNDDNVLKITTVHSSKG | ||||||
Region | 815-1026 | Nuclease activity, interacts with RecD and RecA | ||||
Sequence: SQPIYSFSSTTESQYFTEPFQEISSSSLFPGGSLTGTLIHKLLESLSGNFNASLEEITHKAQTLLKNTILEGFESIISEKIYTAFSTKLPFVSGSFALKDVHPYNIRVEETFLLQENGELWQGIVDLFFEHNNRFFIIDWKTSFLGDEPSLYSPDKLLLYIQRQGLDKQGVLYKRAAKKFLHQFNSSLQIEMAFVFIRGIDDKGNGFLQPSP |
Domain
The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC.
The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA.
Sequence similarities
Belongs to the helicase family. UvrD subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,026
- Mass (Da)117,832
- Last updated2010-07-13 v2
- Checksum4804515055FF6F93
Features
Showing features for sequence uncertainty.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence uncertainty | 125 | L or I | ||||
Sequence: L |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE002160 EMBL· GenBank· DDBJ | AAF38900.2 EMBL· GenBank· DDBJ | Genomic DNA |