Q9PKI5 · KDTA_CHLMU
- Protein3-deoxy-D-manno-octulosonic acid transferase
- GenewaaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids430 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of three 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A. Thus generates the genus-specific LPS epitope of Chlamydia, composed of the trisaccharide alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo.
Catalytic activity
- CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) = alpha-Kdo-(2->6)-lipid IVA (E. coli) + CMP + H+
Pathway
Bacterial outer membrane biogenesis; LPS core biosynthesis.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 66 | Proton acceptor | ||||
Sequence: E | ||||||
Site | 137 | Transition state stabilizer | ||||
Sequence: E | ||||||
Site | 215 | Transition state stabilizer | ||||
Sequence: K | ||||||
Binding site | 274-275 | CMP (UniProtKB | ChEBI) | ||||
Sequence: PR | ||||||
Binding site | 314-316 | CMP (UniProtKB | ChEBI) | ||||
Sequence: MGI | ||||||
Binding site | 341-344 | CMP (UniProtKB | ChEBI) | ||||
Sequence: NLLE |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | Kdo transferase activity | |
Biological Process | lipopolysaccharide core region biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended name3-deoxy-D-manno-octulosonic acid transferase
- EC number
- Short namesKdo transferase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Chlamydiota > Chlamydiia > Chlamydiales > Chlamydiaceae > Chlamydia/Chlamydophila group > Chlamydia
Accessions
- Primary accessionQ9PKI5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 12-32 | Helical; Signal-anchor | ||||
Sequence: AFLVAAFLAAAPRIFYKVVFH |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000080282 | 1-430 | 3-deoxy-D-manno-octulosonic acid transferase | |||
Sequence: MRRWLTSRLYDAFLVAAFLAAAPRIFYKVVFHGKYINSWKIRFGVEKPQVKGEGPLVWFHGASVGEVSLLEPLLKKWRQEFPDWRFVVTACSEAGVYTAQRLYAPLGATVFVLPLDLSCIINPVVRSLSPQVVIFSEGDCWLHFLMGAKKLGAKAFLINGKLSENSCKRFAFLKRLGRSYFAPLDLLVLQDKVYKQRFMQIGIPEDKIQISGNLKTFIETETSINNRSLWRKKLKLSPSDRLIVLGSMHPKDVEVWADVAQHFNKFSTKILWVPRHLEKLKEHARLLEKAGISFGLWSKEDSLLQYDSLIVDAMGILKDLYSAADLAFVGGTFDPLVGGHNLLEPLQKEVPLMFGPHIHSQSVLAELLRTKEVGVSVDKENLLEAVENLLEDEKKRQAYIERGKSFLKNAGTSFEHTWEILKSQIACIKI |
Structure
Family & Domains
Sequence similarities
Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length430
- Mass (Da)48,858
- Last updated2000-10-01 v1
- ChecksumFCB828B9FA796C8A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE002160 EMBL· GenBank· DDBJ | AAF39326.1 EMBL· GenBank· DDBJ | Genomic DNA |