Q9PGD4 · PBPA_XYLFA
- ProteinPenicillin-binding protein 1A
- GenemrcA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids809 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).
Catalytic activity
- [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H+
[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate RHEA-COMP:9602 + CHEBI:60033 = [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate RHEA-COMP:9603 + CHEBI:58405 + CHEBI:15378
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 90 | Proton donor; for transglycosylase activity | ||||
Sequence: E | ||||||
Active site | 460 | Acyl-ester intermediate; for transpeptidase activity | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | penicillin binding | |
Molecular Function | peptidoglycan glycosyltransferase activity | |
Molecular Function | serine-type D-Ala-D-Ala carboxypeptidase activity | |
Biological Process | cell wall organization | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | proteolysis | |
Biological Process | regulation of cell shape | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePenicillin-binding protein 1A
- Short namesPBP-1a; PBP1a
Including 2 domains:
- Recommended namePenicillin-insensitive transglycosylase
- EC number
- Alternative names
- Recommended namePenicillin-sensitive transpeptidase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Lysobacterales > Lysobacteraceae > Xylella
Accessions
- Primary accessionQ9PGD4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-5 | Cytoplasmic | ||||
Sequence: MYRLR | ||||||
Transmembrane | 6-26 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: GWLGWLLTILIILTLVGILAA | ||||||
Topological domain | 27-809 | Periplasmic | ||||
Sequence: GGMYYAISSKLPDIRNLKQVELQEPMYVYSHDGLLMAIFGEIRRYPVQIKEVPEQLKQAFLATEDARFYEHSGIDYKGIARAIWLLATTNEKRVPGGSTITQQVARQFFLNSEYSYKRKLAEILLARKIESQLKKDEILELYLNKSFFGNRAYGIAAAAEFYYGKKLNELNLDEMASLAGIPKFPSSGNPITNPARARERRDLYVLQRMVDLNFITQDQANAAKAIPMHAKPHEPPVQVYAPYAAEMVRQEMIAKYGGAALNKGYHVTTTIDGTLQMAAETSVRNGLALYEHRHRWRGAEQHIEVDANADTATLAAHLSGISSQGGMFPVIVARTTPEGSAVVVRSDRSELTLPPAAARWTGTPLNKLLKRGDVVRVRPGEKWGEWTLEQLPSAQSTLVSLDANNGALRALVGGFSFTANKFNRATQARRQPGSSFKPFVYSAALEKGFNPASIVPDAPVIFRDRRGHTWSPQNDDGKFQGPMRIRDALVQSRNLVSVRLLDAIGVDFARNYITQFGFEENEIPPNLSMSLGTASLTPLSIARGYAVFANGGFRIDPWLIDEIHDREHKLIFKNTPKVACRSCTSANTNVTQSTSQLVDGFNFGAPTKTEPSKPVKNNNEPPQTIPSPDPNTPMAPRAIDERVVFQLVSMMRDVVLRGTATAARSLNRQDIAGKTGSTNEHRDAWFSGFGGPYVTTVWVGRDDFQSLGYREYGGKAALPIWIEYMRTALKDKPIARNVPPQGMTQTTLNGATEWVKNEDINHLQDHDFKLHEEESDEQTFDIF |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000083176 | 1-809 | Penicillin-binding protein 1A | |||
Sequence: MYRLRGWLGWLLTILIILTLVGILAAGGMYYAISSKLPDIRNLKQVELQEPMYVYSHDGLLMAIFGEIRRYPVQIKEVPEQLKQAFLATEDARFYEHSGIDYKGIARAIWLLATTNEKRVPGGSTITQQVARQFFLNSEYSYKRKLAEILLARKIESQLKKDEILELYLNKSFFGNRAYGIAAAAEFYYGKKLNELNLDEMASLAGIPKFPSSGNPITNPARARERRDLYVLQRMVDLNFITQDQANAAKAIPMHAKPHEPPVQVYAPYAAEMVRQEMIAKYGGAALNKGYHVTTTIDGTLQMAAETSVRNGLALYEHRHRWRGAEQHIEVDANADTATLAAHLSGISSQGGMFPVIVARTTPEGSAVVVRSDRSELTLPPAAARWTGTPLNKLLKRGDVVRVRPGEKWGEWTLEQLPSAQSTLVSLDANNGALRALVGGFSFTANKFNRATQARRQPGSSFKPFVYSAALEKGFNPASIVPDAPVIFRDRRGHTWSPQNDDGKFQGPMRIRDALVQSRNLVSVRLLDAIGVDFARNYITQFGFEENEIPPNLSMSLGTASLTPLSIARGYAVFANGGFRIDPWLIDEIHDREHKLIFKNTPKVACRSCTSANTNVTQSTSQLVDGFNFGAPTKTEPSKPVKNNNEPPQTIPSPDPNTPMAPRAIDERVVFQLVSMMRDVVLRGTATAARSLNRQDIAGKTGSTNEHRDAWFSGFGGPYVTTVWVGRDDFQSLGYREYGGKAALPIWIEYMRTALKDKPIARNVPPQGMTQTTLNGATEWVKNEDINHLQDHDFKLHEEESDEQTFDIF |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 52-221 | Transglycosylase | ||||
Sequence: MYVYSHDGLLMAIFGEIRRYPVQIKEVPEQLKQAFLATEDARFYEHSGIDYKGIARAIWLLATTNEKRVPGGSTITQQVARQFFLNSEYSYKRKLAEILLARKIESQLKKDEILELYLNKSFFGNRAYGIAAAAEFYYGKKLNELNLDEMASLAGIPKFPSSGNPITNPA | ||||||
Region | 411-749 | Transpeptidase | ||||
Sequence: EWTLEQLPSAQSTLVSLDANNGALRALVGGFSFTANKFNRATQARRQPGSSFKPFVYSAALEKGFNPASIVPDAPVIFRDRRGHTWSPQNDDGKFQGPMRIRDALVQSRNLVSVRLLDAIGVDFARNYITQFGFEENEIPPNLSMSLGTASLTPLSIARGYAVFANGGFRIDPWLIDEIHDREHKLIFKNTPKVACRSCTSANTNVTQSTSQLVDGFNFGAPTKTEPSKPVKNNNEPPQTIPSPDPNTPMAPRAIDERVVFQLVSMMRDVVLRGTATAARSLNRQDIAGKTGSTNEHRDAWFSGFGGPYVTTVWVGRDDFQSLGYREYGGKAALPIWIE | ||||||
Region | 624-661 | Disordered | ||||
Sequence: VDGFNFGAPTKTEPSKPVKNNNEPPQTIPSPDPNTPMA |
Sequence similarities
In the N-terminal section; belongs to the glycosyltransferase 51 family.
In the C-terminal section; belongs to the transpeptidase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length809
- Mass (Da)90,224
- Last updated2001-06-20 v2
- Checksum9B3207B44BFC4EF3
Sequence caution
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE003849 EMBL· GenBank· DDBJ | AAF83178.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |