Q9PDD9 · MNMA_XYLFA
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids379 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- uridine34 in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 2-thiouridine34 in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H+
RHEA-COMP:11727 CHEBI:65315 Position: 34+ RHEA-COMP:11726 + CHEBI:17499 + CHEBI:30616 = RHEA-COMP:11728 CHEBI:87170 Position: 34+ RHEA-COMP:10131 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 9-16 | ATP (UniProtKB | ChEBI) | |||
Binding site | 35 | ATP (UniProtKB | ChEBI) | |||
Active site | 99 | Nucleophile | |||
Binding site | 123 | ATP (UniProtKB | ChEBI) | |||
Site | 124 | Interaction with tRNA | |||
Active site | 195 | Cysteine persulfide intermediate | |||
Site | 340 | Interaction with tRNA | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Lysobacterales > Lysobacteraceae > Xylella
Accessions
- Primary accessionQ9PDD9
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000121705 | 1-379 | tRNA-specific 2-thiouridylase MnmA | ||
Disulfide bond | 99↔195 | Alternate | |||
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 94-96 | Interaction with target base in tRNA | |||
Region | 145-147 | Interaction with tRNA | |||
Region | 307-308 | Interaction with tRNA | |||
Sequence similarities
Belongs to the MnmA/TRMU family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length379
- Mass (Da)42,975
- Last updated2000-10-01 v1
- ChecksumEF0DEA153D1F377C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE003849 EMBL· GenBank· DDBJ | AAF84249.1 EMBL· GenBank· DDBJ | Genomic DNA |