Q9P7H1 · FLP1_SCHPO
- ProteinTyrosine-protein phosphatase CDC14 homolog
- Geneclp1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids537 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Protein phosphatase which antagonizes mitotic cyclin-dependent kinase cdc2, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Dephosphorylates the mitotic inducer cdc25 for its rapid degradation. Down-regulation of cdc25 activity ensures a prompt inactivation of mitotic cdc2 complexes to trigger cell division. Dephosphorylates also cdc2-phosphorylated nsk1, allowing nsk1-binding to kinetochores and spindle. Dephosphorylates ase1, which is essential for spindle midzone assembly and for continuous extension of the anaphase spindle. Tethered to the contractile ring by mid1, where it dephosphorylates cdc15.
Catalytic activity
- O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 286 | Phosphocysteine intermediate | |||
GO annotations
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTyrosine-protein phosphatase CDC14 homolog
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Taphrinomycotina > Schizosaccharomycetes > Schizosaccharomycetales > Schizosaccharomycetaceae > Schizosaccharomyces
Accessions
- Primary accessionQ9P7H1
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to kinetochores in prometaphase. Cytoplasmic retention is mediated through the binding of rad24. Tethered to the contractile ring by mid1.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 286 | Inactivates phosphatase activity. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 4 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000094874 | 1-537 | Tyrosine-protein phosphatase CDC14 homolog | ||
Modified residue | 453 | Phosphothreonine | |||
Modified residue | 468 | Phosphoserine | |||
Modified residue | 470 | Phosphoserine | |||
Modified residue | 513 | Phosphoserine | |||
Post-translational modification
Phosphorylated by cds1, chk1, pmk1, and cdc2 upon Hydroxylurea and H2O2 stress treatment. Phosphorylation regulates the nucleolar-to-nucleoplasmic transition. Is able to autodephosphorylate.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with ark1 at the kinetochores. Interacts with bir1, cdc25, mid1, nbl1, pic1, and rad24.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q9P7H1 | rad24 P42656 | 3 | EBI-704737, EBI-704791 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 182-345 | Tyrosine-protein phosphatase | |||
Region | 359-537 | Disordered | |||
Compositional bias | 368-384 | Polar residues | |||
Compositional bias | 403-439 | Polar residues | |||
Compositional bias | 447-527 | Polar residues | |||
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length537
- Mass (Da)60,253
- Last updated2000-10-01 v1
- MD5 Checksum372D34C281543350D38C561BD668D335
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 368-384 | Polar residues | |||
Compositional bias | 403-439 | Polar residues | |||
Compositional bias | 447-527 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU329670 EMBL· GenBank· DDBJ | CAB76271.1 EMBL· GenBank· DDBJ | Genomic DNA |