Q9P1Q0 · VPS54_HUMAN
- ProteinVacuolar protein sorting-associated protein 54
- GeneVPS54
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids977 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545).
Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061).
Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameVacuolar protein sorting-associated protein 54
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9P1Q0
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Associates with membranes in an EIPR1-independent manner.
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_052944 | 561 | in dbSNP:rs34015596 | |||
Sequence: S → C | ||||||
Natural variant | VAR_061983 | 912 | in dbSNP:rs11558741 | |||
Sequence: M → I |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 983 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), modified residue.
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000148731 | 1-977 | UniProt | Vacuolar protein sorting-associated protein 54 | |||
Sequence: MASSHSSSPVPQGSSSDVFFKIEVDPSKHIRPVPSLPDVCPKEPTGDSHSLYVAPSLVTDQHRWTVYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEEERLISLVFGLLKQRKLNFLEIYGEKMVITAKNIIKQCVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR | |||||||
Modified residue (large scale data) | 7 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 8 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 8 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 35 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 45 | PRIDE | Phosphothreonine | ||||
Sequence: T | |||||||
Modified residue (large scale data) | 52 | PRIDE | Phosphotyrosine | ||||
Sequence: Y | |||||||
Modified residue (large scale data) | 541 | PRIDE | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54 (PubMed:15878329, PubMed:25799061, PubMed:27440922).
EIPR1 interacts with GARP complex and mediates its recruitment to the trans-Golgi network (PubMed:27440922).
Interacts with VPS51 in an EIPR1-independent manner (PubMed:31721635).
EIPR1 interacts with GARP complex and mediates its recruitment to the trans-Golgi network (PubMed:27440922).
Interacts with VPS51 in an EIPR1-independent manner (PubMed:31721635).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9P1Q0 | VPS53 Q5VIR6 | 4 | EBI-5235744, EBI-2850511 | |
BINARY | Q9P1Q0-4 | CASP6 P55212 | 3 | EBI-25835297, EBI-718729 | |
BINARY | Q9P1Q0-4 | CCK P06307 | 3 | EBI-25835297, EBI-6624398 | |
BINARY | Q9P1Q0-4 | FGFR3 P22607 | 3 | EBI-25835297, EBI-348399 | |
BINARY | Q9P1Q0-4 | GRIN2C Q14957 | 3 | EBI-25835297, EBI-8285963 | |
BINARY | Q9P1Q0-4 | GSN P06396 | 3 | EBI-25835297, EBI-351506 | |
BINARY | Q9P1Q0-4 | HIP1 O00291 | 3 | EBI-25835297, EBI-473886 | |
BINARY | Q9P1Q0-4 | LAMP2 P13473-2 | 3 | EBI-25835297, EBI-21591415 | |
BINARY | Q9P1Q0-4 | Q9Y649 | 3 | EBI-25835297, EBI-25900580 | |
BINARY | Q9P1Q0-4 | RAN P62826 | 3 | EBI-25835297, EBI-286642 | |
BINARY | Q9P1Q0-4 | UBQLN1 Q9UMX0 | 3 | EBI-25835297, EBI-741480 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for coiled coil, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 240-260 | |||||
Sequence: ELQDYLRKTSQAVKMLRDKIA | ||||||
Coiled coil | 480-507 | |||||
Sequence: LDKNQRTRELEEISQQKNAAKDNSLDTE | ||||||
Compositional bias | 535-571 | Polar residues | ||||
Sequence: TSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSS | ||||||
Region | 535-575 | Disordered | ||||
Sequence: TSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPG | ||||||
Coiled coil | 582-603 | |||||
Sequence: SEDMKLTDSELGKLANNIQELL |
Sequence similarities
Belongs to the VPS54 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 5 isoforms produced by Alternative splicing.
Q9P1Q0-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length977
- Mass (Da)110,589
- Last updated2005-05-10 v2
- Checksum3290160E3460C836
Q9P1Q0-2
- Name2
- Differences from canonical
- 964-977: DLDLNMAEIWEQKR → IWT
Q9P1Q0-3
- Name3
Q9P1Q0-4
- Name4
- Differences from canonical
- 46-57: Missing
Q9P1Q0-5
- Name5
- Differences from canonical
- 1-838: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0A0MT48 | A0A0A0MT48_HUMAN | VPS54 | 81 |
Sequence caution
Features
Showing features for alternative sequence, sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_013752 | 1-117 | in isoform 3 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_013751 | 1-838 | in isoform 5 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_013753 | 46-57 | in isoform 4 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_013754 | 118-126 | in isoform 3 | |||
Sequence: TVYQQEISQ → MLPTKNRIK | ||||||
Sequence conflict | 130 | in Ref. 1; AAF37319 | ||||
Sequence: I → V | ||||||
Alternative sequence | VSP_013755 | 380-415 | in isoform 3 | |||
Sequence: Missing | ||||||
Sequence conflict | 526 | in Ref. 1; AAF37319 | ||||
Sequence: E → K | ||||||
Compositional bias | 535-571 | Polar residues | ||||
Sequence: TSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSS | ||||||
Sequence conflict | 601 | in Ref. 1; AAF37319 | ||||
Sequence: E → G | ||||||
Sequence conflict | 643 | in Ref. 1; AAF37319 | ||||
Sequence: E → G | ||||||
Sequence conflict | 650 | in Ref. 1; AAF37319 | ||||
Sequence: E → A | ||||||
Sequence conflict | 677-679 | in Ref. 1; AAF37319 | ||||
Sequence: EER → VGG | ||||||
Sequence conflict | 727 | in Ref. 5; BAA92134 | ||||
Sequence: K → E | ||||||
Sequence conflict | 844 | in Ref. 4; AAH41868 | ||||
Sequence: D → G | ||||||
Sequence conflict | 904 | in Ref. 4; AAH41868 | ||||
Sequence: L → H | ||||||
Alternative sequence | VSP_013756 | 964-977 | in isoform 2 | |||
Sequence: DLDLNMAEIWEQKR → IWT |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF102177 EMBL· GenBank· DDBJ | AAF37319.1 EMBL· GenBank· DDBJ | mRNA | ||
AY444798 EMBL· GenBank· DDBJ | AAS20945.1 EMBL· GenBank· DDBJ | mRNA | ||
AL137604 EMBL· GenBank· DDBJ | CAB70837.1 EMBL· GenBank· DDBJ | mRNA | ||
AL359939 EMBL· GenBank· DDBJ | CAB95772.1 EMBL· GenBank· DDBJ | mRNA | ||
CR749701 EMBL· GenBank· DDBJ | CAH18479.1 EMBL· GenBank· DDBJ | mRNA | ||
BC030275 EMBL· GenBank· DDBJ | AAH30275.1 EMBL· GenBank· DDBJ | mRNA | ||
BC041868 EMBL· GenBank· DDBJ | AAH41868.1 EMBL· GenBank· DDBJ | mRNA | ||
AK002205 EMBL· GenBank· DDBJ | BAA92134.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |