Q9NX76 · CKLF6_HUMAN
- ProteinCKLF-like MARVEL transmembrane domain-containing protein 6
- GeneCMTM6
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids183 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Master regulator of recycling and plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Associates with both constitutive and IFNG-induced PD-L1/CD274 at recycling endosomes, where it protects PD-L1/CD274 from being targeted for lysosomal degradation, likely by preventing its STUB1-mediated ubiquitination. May stabilize PD-L1/CD274 expression on antigen presenting cells and potentiates inhibitory signaling by PDCD1/CD279, its receptor on T-cells, ultimately triggering T-cell anergy.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | azurophil granule membrane | |
Cellular Component | early endosome membrane | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Cellular Component | recycling endosome membrane | |
Cellular Component | specific granule membrane | |
Biological Process | endocytic recycling | |
Biological Process | protein transport | |
Biological Process | regulation of protein stability |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCKLF-like MARVEL transmembrane domain-containing protein 6
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9NX76
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Early endosome membrane ; Multi-pass membrane protein
Recycling endosome membrane ; Multi-pass membrane protein
Note: Co-localizes with PD-L1/CD274 in the plasma membrane and in recycling endosomes.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-39 | Cytoplasmic | ||||
Sequence: MENGAVYSPTTEEDPGPARGPRSGLAAYFFMGRLPLLRR | ||||||
Transmembrane | 40-60 | Helical | ||||
Sequence: VLKGLQLLLSLLAFICEEVVS | ||||||
Topological domain | 61-67 | Extracellular | ||||
Sequence: QCTLCGG | ||||||
Transmembrane | 68-88 | Helical | ||||
Sequence: LYFFEFVSCSAFLLSLLILIV | ||||||
Topological domain | 89-106 | Cytoplasmic | ||||
Sequence: YCTPFYERVDTTKVKSSD | ||||||
Transmembrane | 107-127 | Helical | ||||
Sequence: FYITLGTGCVFLLASIIFVST | ||||||
Topological domain | 128-134 | Extracellular | ||||
Sequence: HDRTSAE | ||||||
Transmembrane | 135-155 | Helical | ||||
Sequence: IAAIVFGFIASFMFLLDFITM | ||||||
Topological domain | 156-183 | Cytoplasmic | ||||
Sequence: LYEKRQESQLRKPENTTRAEALTEPLNA |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_061998 | 91 | in dbSNP:rs35574803 | |||
Sequence: T → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 184 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for modified residue, chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Modified residue | 1 | UniProt | N-acetylmethionine | ||||
Sequence: M | |||||||
Chain | PRO_0000186107 | 1-183 | UniProt | CKLF-like MARVEL transmembrane domain-containing protein 6 | |||
Sequence: MENGAVYSPTTEEDPGPARGPRSGLAAYFFMGRLPLLRRVLKGLQLLLSLLAFICEEVVSQCTLCGGLYFFEFVSCSAFLLSLLILIVYCTPFYERVDTTKVKSSDFYITLGTGCVFLLASIIFVSTHDRTSAEIAAIVFGFIASFMFLLDFITMLYEKRQESQLRKPENTTRAEALTEPLNA | |||||||
Modified residue (large scale data) | 7 | PRIDE | Phosphotyrosine | ||||
Sequence: Y | |||||||
Modified residue | 8 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 8 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 171 | UniProt | Phosphothreonine | ||||
Sequence: T | |||||||
Modified residue (large scale data) | 178 | PRIDE | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with PD-L1/CD274 (via transmembrane domain); the interaction is direct (PubMed:28813410, PubMed:28813417).
Interacts with CMTM4 (PubMed:28813410).
Interacts with CD58, ARG1, ENO1 and TMPO (PubMed:28813417).
Interacts with CMTM4 (PubMed:28813410).
Interacts with CD58, ARG1, ENO1 and TMPO (PubMed:28813417).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9NX76 | APOA5 Q6Q788 | 3 | EBI-1054315, EBI-3936819 | |
BINARY | Q9NX76 | BPIFA2 Q96DR5 | 3 | EBI-1054315, EBI-10284754 | |
BINARY | Q9NX76 | CD160 O95971 | 3 | EBI-1054315, EBI-4314390 | |
BINARY | Q9NX76 | CD274 Q9NZQ7 | 14 | EBI-1054315, EBI-4314282 | |
BINARY | Q9NX76 | CD40 P25942 | 4 | EBI-1054315, EBI-525714 | |
BINARY | Q9NX76 | CIAO2A Q9H5X1 | 3 | EBI-1054315, EBI-752069 | |
BINARY | Q9NX76 | COQ8A Q8NI60 | 3 | EBI-1054315, EBI-745535 | |
BINARY | Q9NX76 | ECPAS Q5VYK3 | 3 | EBI-1054315, EBI-521451 | |
BINARY | Q9NX76 | EHHADH Q08426 | 3 | EBI-1054315, EBI-2339219 | |
BINARY | Q9NX76 | MIEF1 Q9NQG6 | 3 | EBI-1054315, EBI-740987 | |
BINARY | Q9NX76 | RBFA Q8N0V3 | 3 | EBI-1054315, EBI-3232108 | |
BINARY | Q9NX76 | RBMX P38159 | 3 | EBI-1054315, EBI-743526 | |
BINARY | Q9NX76 | RUSC1 Q9BVN2 | 3 | EBI-1054315, EBI-6257312 | |
BINARY | Q9NX76 | SNRPB2 P08579 | 3 | EBI-1054315, EBI-1053651 | |
BINARY | Q9NX76 | SPG21 Q9NZD8 | 3 | EBI-1054315, EBI-742688 | |
BINARY | Q9NX76 | TCEA2 Q15560 | 3 | EBI-1054315, EBI-710310 | |
BINARY | Q9NX76 | TMED8 Q6PL24 | 3 | EBI-1054315, EBI-11603430 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 33-160 | MARVEL | ||||
Sequence: RLPLLRRVLKGLQLLLSLLAFICEEVVSQCTLCGGLYFFEFVSCSAFLLSLLILIVYCTPFYERVDTTKVKSSDFYITLGTGCVFLLASIIFVSTHDRTSAEIAAIVFGFIASFMFLLDFITMLYEKR |
Sequence similarities
Belongs to the chemokine-like factor family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length183
- Mass (Da)20,419
- Last updated2000-10-01 v1
- Checksum43DAEB652A6B50B4
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF479261 EMBL· GenBank· DDBJ | AAN73039.1 EMBL· GenBank· DDBJ | mRNA | ||
AK000403 EMBL· GenBank· DDBJ | BAA91141.1 EMBL· GenBank· DDBJ | mRNA | ||
CR457231 EMBL· GenBank· DDBJ | CAG33512.1 EMBL· GenBank· DDBJ | mRNA | ||
CH471055 EMBL· GenBank· DDBJ | EAW64429.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC002797 EMBL· GenBank· DDBJ | AAH02797.1 EMBL· GenBank· DDBJ | mRNA |