Q9NUL7 · DDX28_HUMAN
- ProteinProbable ATP-dependent RNA helicase DDX28
- GeneDDX28
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids540 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays an essential role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function (PubMed:25683708, PubMed:25683715).
May be involved in RNA processing or transport. Has RNA and Mg2+-dependent ATPase activity (PubMed:11350955).
May be involved in RNA processing or transport. Has RNA and Mg2+-dependent ATPase activity (PubMed:11350955).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrial nucleoid | |
Cellular Component | mitochondrion | |
Cellular Component | nucleolus | |
Cellular Component | nucleoplasm | |
Cellular Component | ribonucleoprotein granule | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Molecular Function | rRNA binding | |
Biological Process | mitochondrial large ribosomal subunit assembly |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable ATP-dependent RNA helicase DDX28
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9NUL7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Transported between these two compartments. Nuclear localization depends on active RNA polymerase II transcription. Localizes to mitochondrial RNA granules found in close proximity to the mitochondrial nucleoids.
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_052163 | 4 | in dbSNP:rs237831 | |||
Sequence: T → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 739 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000055033 | 1-540 | UniProt | Probable ATP-dependent RNA helicase DDX28 | |||
Sequence: MALTRPVRLFSLVTRLLLAPRRGLTVRSPDEPLPVVRIPVALQRQLEQRQSRRRNLPRPVLVRPGPLLVSARRPELNQPARLTLGRWERAPLASQGWKSRRARRDHFSIERAQQEAPAVRKLSSKGSFADLGLEPRVLHALQEAAPEVVQPTTVQSSTIPSLLRGRHVVCAAETGSGKTLSYLLPLLQRLLGQPSLDSLPIPAPRGLVLVPSRELAQQVRAVAQPLGRSLGLLVRDLEGGHGMRRIRLQLSRQPSADVLVATPGALWKALKSRLISLEQLSFLVLDEADTLLDESFLELVDYILEKSHIAEGPADLEDPFNPKAQLVLVGATFPEGVGQLLNKVASPDAVTTITSSKLHCIMPHVKQTFLRLKGADKVAELVHILKHRDRAERTGPSGTVLVFCNSSSTVNWLGYILDDHKIQHLRLQGQMPALMRVGIFQSFQKSSRDILLCTDIASRGLDSTGVELVVNYDFPPTLQDYIHRAGRVGRVGSEVPGTVISFVTHPWDVSLVQKIELAARRRRSLPGLASSVKEPLPQAT | |||||||
Modified residue (large scale data) | 28 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 127 | PRIDE | Phosphoserine | ||||
Sequence: S |
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 3-18 | Mitochondrial targeting signal | ||||
Sequence: LTRPVRLFSLVTRLLL | ||||||
Motif | 126-156 | Q motif | ||||
Sequence: GSFADLGLEPRVLHALQEAAPEVVQPTTVQS | ||||||
Domain | 159-351 | Helicase ATP-binding | ||||
Sequence: IPSLLRGRHVVCAAETGSGKTLSYLLPLLQRLLGQPSLDSLPIPAPRGLVLVPSRELAQQVRAVAQPLGRSLGLLVRDLEGGHGMRRIRLQLSRQPSADVLVATPGALWKALKSRLISLEQLSFLVLDEADTLLDESFLELVDYILEKSHIAEGPADLEDPFNPKAQLVLVGATFPEGVGQLLNKVASPDAVT | ||||||
Motif | 180-191 | Nuclear export signal | ||||
Sequence: LSYLLPLLQRLL | ||||||
Motif | 286-289 | DEAD | ||||
Sequence: DEAD | ||||||
Domain | 377-536 | Helicase C-terminal | ||||
Sequence: KVAELVHILKHRDRAERTGPSGTVLVFCNSSSTVNWLGYILDDHKIQHLRLQGQMPALMRVGIFQSFQKSSRDILLCTDIASRGLDSTGVELVVNYDFPPTLQDYIHRAGRVGRVGSEVPGTVISFVTHPWDVSLVQKIELAARRRRSLPGLASSVKEPL | ||||||
Motif | 520-523 | Nuclear localization signal | ||||
Sequence: RRRR |
Sequence similarities
Belongs to the DEAD box helicase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length540
- Mass (Da)59,581
- Last updated2010-05-18 v2
- Checksum1FAA4477853893B5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF329821 EMBL· GenBank· DDBJ | AAG59833.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK002144 EMBL· GenBank· DDBJ | BAA92106.1 EMBL· GenBank· DDBJ | mRNA | ||
AC130462 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC024273 EMBL· GenBank· DDBJ | AAH24273.1 EMBL· GenBank· DDBJ | mRNA |