Q9NPZ5 · B3GA2_HUMAN
- ProteinGalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2
- GeneB3GAT2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids323 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins.
Catalytic activity
- 3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + H+ + UDP
Cofactor
Pathway
Protein modification; protein glycosylation.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 87-89 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: PTY | ||||||
Binding site | 118 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 155 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 160 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 185-187 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: DDD | ||||||
Binding site | 187 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Site | 218 | Interaction with galactose moiety of substrate glycoprotein | ||||
Sequence: E | ||||||
Active site | 273 | Proton donor/acceptor | ||||
Sequence: E | ||||||
Binding site | 300-302 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: HTR | ||||||
Site | 310 | Interaction with galactose moiety of substrate glycoprotein | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Molecular Function | galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | carbohydrate biosynthetic process | |
Biological Process | carbohydrate metabolic process | |
Biological Process | chondroitin sulfate proteoglycan biosynthetic process | |
Biological Process | protein glycosylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameGalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9NPZ5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-2 | Cytoplasmic | ||||
Sequence: MK | ||||||
Transmembrane | 3-23 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: SALFTRFFILLPWILIVIIML | ||||||
Topological domain | 24-323 | Lumenal | ||||
Sequence: DVDTRRPVPPLTPRPYFSPYAVGRGGARLPLRRGGPAHGTQKRNQSRPQPQPEPQLPTIYAITPTYSRPVQKAELTRLANTFRQVAQLHWILVEDAAARSELVSRFLARAGLPSTHLHVPTPRRYKRPGLPRATEQRNAGLAWLRQRHQHQRAQPGVLFFADDDNTYSLELFQEMRTTRKVSVWPVGLVGGRRYERPLVENGKVVGWYTGWRADRPFAIDMAGFAVSLQVILSNPKAVFKRRGSQPGMQESDFLKQITTVEELEPKANNCTKVLVWHTRTEKVNLANEPKYHLDTVKIEV |
Keywords
- Cellular component
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 438 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000195172 | 1-323 | Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 | |||
Sequence: MKSALFTRFFILLPWILIVIIMLDVDTRRPVPPLTPRPYFSPYAVGRGGARLPLRRGGPAHGTQKRNQSRPQPQPEPQLPTIYAITPTYSRPVQKAELTRLANTFRQVAQLHWILVEDAAARSELVSRFLARAGLPSTHLHVPTPRRYKRPGLPRATEQRNAGLAWLRQRHQHQRAQPGVLFFADDDNTYSLELFQEMRTTRKVSVWPVGLVGGRRYERPLVENGKVVGWYTGWRADRPFAIDMAGFAVSLQVILSNPKAVFKRRGSQPGMQESDFLKQITTVEELEPKANNCTKVLVWHTRTEKVNLANEPKYHLDTVKIEV | ||||||
Glycosylation | 67 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 292 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the trachea, retina, spinal cord, hippocampus and other brain regions, and, at lower levels, in testis and ovary.
Gene expression databases
Organism-specific databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 51-80 | Disordered | ||||
Sequence: RLPLRRGGPAHGTQKRNQSRPQPQPEPQLP | ||||||
Region | 234-243 | Interaction with galactose moiety of substrate glycoprotein | ||||
Sequence: WRADRPFAID |
Sequence similarities
Belongs to the glycosyltransferase 43 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length323
- Mass (Da)36,919
- Last updated2001-06-01 v2
- Checksum85058D52D2D28463
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A087WXU9 | A0A087WXU9_HUMAN | B3GAT2 | 251 |
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY070019 EMBL· GenBank· DDBJ | AAL57718.1 EMBL· GenBank· DDBJ | mRNA | ||
AY070110 EMBL· GenBank· DDBJ | AAL58977.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY070108 EMBL· GenBank· DDBJ | AAL58977.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY070109 EMBL· GenBank· DDBJ | AAL58977.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB075843 EMBL· GenBank· DDBJ | BAB85549.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AL121961 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL450320 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AK055248 EMBL· GenBank· DDBJ | BAB70889.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |