Q9N2V6 · GPA16_CAEEL

  • Protein
    Guanine nucleotide-binding protein alpha-16 subunit
  • Gene
    gpa-16
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. In the 1-cell embryo, probably together with goa-1, controls nuclear rotation and spindle elongation during mitosis (PubMed:14534135).
During the first embryonic cell divisons, plays a role in gpr-1/2 cortical localization and in the proper orientation of EMS blastomere mitotic spindle (PubMed:14534135).

Features

Showing features for binding site.

135750100150200250300350
TypeIDPosition(s)Description
Binding site40-47GTP (UniProtKB | ChEBI)
Binding site47Mg2+ (UniProtKB | ChEBI)
Binding site175-181GTP (UniProtKB | ChEBI)
Binding site181Mg2+ (UniProtKB | ChEBI)
Binding site200-204GTP (UniProtKB | ChEBI)
Binding site269-272GTP (UniProtKB | ChEBI)
Binding site329GTP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell cortex
Cellular Componentcytoplasm
Cellular Componentheterotrimeric G-protein complex
Molecular FunctionG protein-coupled receptor binding
Molecular FunctionG-protein beta/gamma-subunit complex binding
Molecular FunctionGDP binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionmetal ion binding
Biological Processadenylate cyclase-modulating G protein-coupled receptor signaling pathway
Biological Processcell division
Biological Processembryonic axis specification
Biological Processestablishment of mitotic spindle orientation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Guanine nucleotide-binding protein alpha-16 subunit

Gene names

    • Name
      gpa-16
    • Synonyms
      spn-1
    • ORF names
      Y95B8A.5

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q9N2V6

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Simultaneous RNAi-mediated knockdown of both gpa-16 and goa-1 causes, in the one-cell embryo, a lack of nuclear rocking movements from prophase to metaphase and symmetric spindle elongation without transversal oscillations of the poles during anaphase. At the 2-cell stage embryo, nuclei are mispositioned and fail to exhibit nuclear rotation. In addition, causes a loss of gpr-1/2 cortical localization in 2-cell and 4-cell stage embryos.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis202In it143ts; temperature sensitive mutant. 70 percent of embryonic lethality. In 31 percent of EMS blastomeres, loss of nuclear rotation resulting in the transversal localization of the mitotic spindle.

PTM/Processing

Features

Showing features for initiator methionine, lipidation, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Lipidation2N-myristoyl glycine
ChainPRO_00002036572-357Guanine nucleotide-binding protein alpha-16 subunit
Lipidation3S-palmitoyl cysteine

Keywords

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q9N2V6gpr-1 Q95QJ72EBI-1005005, EBI-316069
BINARY Q9N2V6ric-8 Q9GSX9-12EBI-1005005, EBI-1004494

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain32-357G-alpha
Region35-48G1 motif
Region173-181G2 motif
Region196-205G3 motif
Region265-272G4 motif
Region327-332G5 motif

Sequence similarities

Belongs to the G-alpha family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    357
  • Mass (Da)
    41,328
  • Last updated
    2007-01-23 v3
  • Checksum
    C0CDB733D06EE6C9
MGCIMSQEDEAAKRRSKKIDRLLKEDGENSMRTIKLLLLGAGESGKSTILKQMRIIHDVGYTTEERKVFRGVVYGNIILSLNAIIHAMEQLKISFTTLDHESDARKLLMFSTTGEEDELPEELVVLMKSVWSDSGIQKALERSREYQLNDSAGYYLSQLDRICAPNYIPTQDDILRTRIKTTGIVETQFVYKDRLFLVFDVGGQRSERKKWIHCFEDVTALIFCVALSEYDMVLVEDCQTNRMRESLKLFDSICNNKWFVETSIILFLNKKDLFEEKIVRSPLTHCFPEYTGANNYEEASAYIQQQFEDMNKRTTGEKNQEIYTQFTCATDTNNIRFVFDAVTDIIIRDNLRTCGLY

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
V6CLF2V6CLF2_CAEELgpa-16115
V6CK09V6CK09_CAEELgpa-16126

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY008138
EMBL· GenBank· DDBJ
AAG32091.1
EMBL· GenBank· DDBJ
mRNA
FO081787
EMBL· GenBank· DDBJ
CCD73543.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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