Q9N2V6 · GPA16_CAEEL
- ProteinGuanine nucleotide-binding protein alpha-16 subunit
- Genegpa-16
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids357 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. In the 1-cell embryo, probably together with goa-1, controls nuclear rotation and spindle elongation during mitosis (PubMed:14534135).
During the first embryonic cell divisons, plays a role in gpr-1/2 cortical localization and in the proper orientation of EMS blastomere mitotic spindle (PubMed:14534135).
During the first embryonic cell divisons, plays a role in gpr-1/2 cortical localization and in the proper orientation of EMS blastomere mitotic spindle (PubMed:14534135).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 40-47 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GAGESGKS | ||||||
Binding site | 47 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 175-181 | GTP (UniProtKB | ChEBI) | ||||
Sequence: LRTRIKT | ||||||
Binding site | 181 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 200-204 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DVGGQ | ||||||
Binding site | 269-272 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NKKD | ||||||
Binding site | 329 | GTP (UniProtKB | ChEBI) | ||||
Sequence: A |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cell cortex | |
Cellular Component | cytoplasm | |
Cellular Component | heterotrimeric G-protein complex | |
Molecular Function | G protein-coupled receptor binding | |
Molecular Function | G-protein beta/gamma-subunit complex binding | |
Molecular Function | GDP binding | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | adenylate cyclase-modulating G protein-coupled receptor signaling pathway | |
Biological Process | cell division | |
Biological Process | embryonic axis specification | |
Biological Process | establishment of mitotic spindle orientation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGuanine nucleotide-binding protein alpha-16 subunit
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ9N2V6
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Simultaneous RNAi-mediated knockdown of both gpa-16 and goa-1 causes, in the one-cell embryo, a lack of nuclear rocking movements from prophase to metaphase and symmetric spindle elongation without transversal oscillations of the poles during anaphase. At the 2-cell stage embryo, nuclei are mispositioned and fail to exhibit nuclear rotation. In addition, causes a loss of gpr-1/2 cortical localization in 2-cell and 4-cell stage embryos.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 202 | In it143ts; temperature sensitive mutant. 70 percent of embryonic lethality. In 31 percent of EMS blastomeres, loss of nuclear rotation resulting in the transversal localization of the mitotic spindle. | ||||
Sequence: G → D |
PTM/Processing
Features
Showing features for initiator methionine, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Lipidation | 2 | N-myristoyl glycine | ||||
Sequence: G | ||||||
Chain | PRO_0000203657 | 2-357 | Guanine nucleotide-binding protein alpha-16 subunit | |||
Sequence: GCIMSQEDEAAKRRSKKIDRLLKEDGENSMRTIKLLLLGAGESGKSTILKQMRIIHDVGYTTEERKVFRGVVYGNIILSLNAIIHAMEQLKISFTTLDHESDARKLLMFSTTGEEDELPEELVVLMKSVWSDSGIQKALERSREYQLNDSAGYYLSQLDRICAPNYIPTQDDILRTRIKTTGIVETQFVYKDRLFLVFDVGGQRSERKKWIHCFEDVTALIFCVALSEYDMVLVEDCQTNRMRESLKLFDSICNNKWFVETSIILFLNKKDLFEEKIVRSPLTHCFPEYTGANNYEEASAYIQQQFEDMNKRTTGEKNQEIYTQFTCATDTNNIRFVFDAVTDIIIRDNLRTCGLY | ||||||
Lipidation | 3 | S-palmitoyl cysteine | ||||
Sequence: C |
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9N2V6 | gpr-1 Q95QJ7 | 2 | EBI-1005005, EBI-316069 | |
BINARY | Q9N2V6 | ric-8 Q9GSX9-1 | 2 | EBI-1005005, EBI-1004494 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 32-357 | G-alpha | ||||
Sequence: RTIKLLLLGAGESGKSTILKQMRIIHDVGYTTEERKVFRGVVYGNIILSLNAIIHAMEQLKISFTTLDHESDARKLLMFSTTGEEDELPEELVVLMKSVWSDSGIQKALERSREYQLNDSAGYYLSQLDRICAPNYIPTQDDILRTRIKTTGIVETQFVYKDRLFLVFDVGGQRSERKKWIHCFEDVTALIFCVALSEYDMVLVEDCQTNRMRESLKLFDSICNNKWFVETSIILFLNKKDLFEEKIVRSPLTHCFPEYTGANNYEEASAYIQQQFEDMNKRTTGEKNQEIYTQFTCATDTNNIRFVFDAVTDIIIRDNLRTCGLY | ||||||
Region | 35-48 | G1 motif | ||||
Sequence: KLLLLGAGESGKST | ||||||
Region | 173-181 | G2 motif | ||||
Sequence: DILRTRIKT | ||||||
Region | 196-205 | G3 motif | ||||
Sequence: FLVFDVGGQR | ||||||
Region | 265-272 | G4 motif | ||||
Sequence: ILFLNKKD | ||||||
Region | 327-332 | G5 motif | ||||
Sequence: TCATDT |
Sequence similarities
Belongs to the G-alpha family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length357
- Mass (Da)41,328
- Last updated2007-01-23 v3
- ChecksumC0CDB733D06EE6C9
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY008138 EMBL· GenBank· DDBJ | AAG32091.1 EMBL· GenBank· DDBJ | mRNA | ||
FO081787 EMBL· GenBank· DDBJ | CCD73543.1 EMBL· GenBank· DDBJ | Genomic DNA |