Q9N290 · A4GAT_GORGO
- ProteinLactosylceramide 4-alpha-galactosyltransferase
- GeneA4GALT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids327 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of galactose from UDP-alpha-D-galactose to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)). Also able to transfer galactose to galactosylceramide/beta-D-Gal-(1<->1')-Cer. Globoside Gb3Cer is a glycosphingolipid of the globo serie, one of the major types of neutral root structures of glycosphingolipids, that constitute a significant portion of mammalian cell membranes.
Catalytic activity
- a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP-alpha-D-galactose = a globoside Gb3Cer (d18:1(4E)) + H+ + UDPThis reaction proceeds in the forward direction.
- beta-D-Gal-(1<->1')-Cer + UDP-alpha-D-galactose = alpha-D-Gal-(1->4)-beta-D-Gal-(1<->1')-Cer + H+ + UDPThis reaction proceeds in the forward direction.
Pathway
Glycolipid biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Molecular Function | acetylglucosaminyltransferase activity | |
Molecular Function | galactosyltransferase activity | |
Molecular Function | lactosylceramide 4-alpha-galactosyltransferase activity | |
Biological Process | lipid metabolic process | |
Biological Process | protein O-linked glycosylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLactosylceramide 4-alpha-galactosyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Gorilla
Accessions
- Primary accessionQ9N290
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000080577 | 1-327 | Lactosylceramide 4-alpha-galactosyltransferase | |||
Sequence: GFKFTFFVSIMIYWHVVGEPKEKGQLYNLPAEIPCPTLAPPTPPSHGPAPGNIFFLETSDRTNPNFLFMCSVESAARTHPESHVLVLMKGLPGGNASLPRHLGISLLSCFPNVQMLPLDLRELFRDTPLADWYAAVQGRWEPYLLPVLSDASRIALMWKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYVLNGAFLAFERXHEFMALCMXDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEELPRLFSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAMKMYL |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 166-168 | DXD motif | ||||
Sequence: DTD |
Domain
The conserved DXD motif is involved in enzyme activity.
Sequence similarities
Belongs to the glycosyltransferase 32 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusFragment
- Length327
- Mass (Da)37,394
- Last updated2000-10-01 v1
- ChecksumED9A9A37A19F66B8
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: G |
Keywords
- Technical term