Q9MA75 · VIP1_ARATH
- ProteinTranscription factor VIP1
- GeneVIP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids341 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription activator that binds specifically to the VIP1 response elements (VREs) DNA sequence 5'-ACNGCT-3' found in some stress genes (e.g. TRX8 and MYB44), when phosphorylated/activated by MPK3. Required for Agrobacterium VirE2 nuclear import and tumorigenicity. Promotes transient expression of T-DNA in early stages by interacting with VirE2 in complex with the T-DNA and facilitating its translocation to the nucleus, and mediates stable genetic transformation by Agrobacterium by binding H2A histone. Prevents cell differentiation and shoot formation. Limits sulfate utilization efficiency (SUE) and sulfate uptake, especially in low-sulfur conditions (PubMed:11432846, PubMed:12124400, PubMed:15108305, PubMed:15824315, PubMed:17947581, PubMed:19820165, PubMed:20547563).
Plays a role in osmosensory response by binding to the 5'-AGCTGT/G-3' DNA sequence found in the promoters of the hypoosmolarity-responsive genes CYP707A1 and CYP707A3 (PubMed:22452852, PubMed:25093810).
Involved in the negative regulation of touch-induced root bending and salt-dependent root bending (PubMed:27208231, PubMed:30010769, PubMed:31504762).
Plays a role in osmosensory response by binding to the 5'-AGCTGT/G-3' DNA sequence found in the promoters of the hypoosmolarity-responsive genes CYP707A1 and CYP707A3 (PubMed:22452852, PubMed:25093810).
Involved in the negative regulation of touch-induced root bending and salt-dependent root bending (PubMed:27208231, PubMed:30010769, PubMed:31504762).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | chromatin binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | mitogen-activated protein kinase binding | |
Molecular Function | nucleic acid binding | |
Molecular Function | sequence-specific DNA binding | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | cellular response to sulfate starvation | |
Biological Process | defense response | |
Biological Process | DNA-mediated transformation | |
Biological Process | import into nucleus | |
Biological Process | negative regulation of cell differentiation | |
Biological Process | osmosensory signaling pathway | |
Biological Process | response to osmotic stress | |
Biological Process | sulfate transport | |
Biological Process | thigmotropism |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameTranscription factor VIP1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9MA75
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
Enhanced low sulfur tolerance with higher rate of sulfate uptake at low sulfate levels. Improved tolerance to heavy metal (e.g. CdCl2) and oxidative stress (e.g. paraquat).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 79 | Cytoplasmic and nuclear. | ||||
Sequence: S → A | ||||||
Mutagenesis | 79 | Only nuclear. | ||||
Sequence: S → D | ||||||
Mutagenesis | 163-341 | Transient T-DNA transformation end expression, but impaired stable genetic transformation by Agrobacterium, loss of multimerization, and abolished interaction with histone H2A. | ||||
Sequence: Missing | ||||||
Mutagenesis | 212 | Impaired VIP1 response elements (VREs) DNA-binding and altered subsequent transcription activation. | ||||
Sequence: K → R or T |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 33 variants from UniProt as well as other sources including ClinVar and dbSNP.
Keywords
- Disease
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000405593 | 1-341 | Transcription factor VIP1 | |||
Sequence: MEGGGRGPNQTILSEIEHMPEAPRQRISHHRRARSETFFSGESIDDLLLFDPSDIDFSSLDFLNAPPPPQQSQQQPQASPMSVDSEETSSNGVVPPNSLPPKPEARFGRHVRSFSVDSDFFDDLGVTEEKFIATSSGEKKKGNHHHSRSNSMDGEMSSASFNIESILASVSGKDSGKKNMGMGGDRLAELALLDPKRAKRILANRQSAARSKERKIRYTGELERKVQTLQNEATTLSAQVTMLQRGTSELNTENKHLKMRLQALEQQAELRDALNEALRDELNRLKVVAGEIPQGNGNSYNRAQFSSQQSAMNQFGNKTNQQMSTNGQPSLPSYMDFTKRG | ||||||
Modified residue | 79 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated by MPK3. This phosphorylation promotes nuclear localization.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Mostly expressed in dividing cells, present in leaves, roots and seedlings.
Induction
Transcriptionally activated during the acquisition of pluripotentiality (in protoplasts) by pericentromeric chromatin decondensation and DNA demethylation. Targeted to degradation by the proteasome by VBF and Agrobacterium virF in SCF(VBF) and SCF(virF) E3 ubiquitin ligase complexes after mediating T-DNA translocation to the nucleus.
Gene expression databases
Interaction
Subunit
Forms homomultimers. Interacts with Agrobacterium tumefaciens VirE2 and mediates its translocation to the host nucleus. Binds to VIP2. Forms a complex made of Agrobacterium VirE2, VIP1, VIP2 and single-stranded DNA (ssDNA). The interaction with KAP1 mediates its nuclear import. Binds to the H2A histone RAT5. Interacts with MPK3 and Agrobacterium virF. Forms a complex made of VIP1, VBF and Agrobacterium virE2. Interacts with SCF(VBF) E3 ubiquitin ligase complex. Binds directly to VBF. Forms heterodimers with BZIP34 and BZIP61.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | Q9MA75 | virF P15597 | 3 | EBI-606057, EBI-605118 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-33 | Disordered | ||||
Sequence: MEGGGRGPNQTILSEIEHMPEAPRQRISHHRRA | ||||||
Region | 1-162 | Necessary and sufficient for transient T-DNA transformation end expression | ||||
Sequence: MEGGGRGPNQTILSEIEHMPEAPRQRISHHRRARSETFFSGESIDDLLLFDPSDIDFSSLDFLNAPPPPQQSQQQPQASPMSVDSEETSSNGVVPPNSLPPKPEARFGRHVRSFSVDSDFFDDLGVTEEKFIATSSGEKKKGNHHHSRSNSMDGEMSSASFN | ||||||
Compositional bias | 19-33 | Basic and acidic residues | ||||
Sequence: MPEAPRQRISHHRRA | ||||||
Region | 59-106 | Disordered | ||||
Sequence: SLDFLNAPPPPQQSQQQPQASPMSVDSEETSSNGVVPPNSLPPKPEAR | ||||||
Compositional bias | 75-95 | Polar residues | ||||
Sequence: QPQASPMSVDSEETSSNGVVP | ||||||
Region | 135-156 | Disordered | ||||
Sequence: SSGEKKKGNHHHSRSNSMDGEM | ||||||
Region | 163-341 | Involved in homomultimerization and histone H2A binding | ||||
Sequence: IESILASVSGKDSGKKNMGMGGDRLAELALLDPKRAKRILANRQSAARSKERKIRYTGELERKVQTLQNEATTLSAQVTMLQRGTSELNTENKHLKMRLQALEQQAELRDALNEALRDELNRLKVVAGEIPQGNGNSYNRAQFSSQQSAMNQFGNKTNQQMSTNGQPSLPSYMDFTKRG | ||||||
Domain | 194-257 | bZIP | ||||
Sequence: DPKRAKRILANRQSAARSKERKIRYTGELERKVQTLQNEATTLSAQVTMLQRGTSELNTENKHL | ||||||
Region | 196-217 | Basic motif | ||||
Sequence: KRAKRILANRQSAARSKERKIR | ||||||
Motif | 198-205 | Nuclear localization signal | ||||
Sequence: AKRILANR | ||||||
Region | 222-257 | Leucine-zipper | ||||
Sequence: LERKVQTLQNEATTLSAQVTMLQRGTSELNTENKHL | ||||||
Compositional bias | 307-333 | Polar residues | ||||
Sequence: SQQSAMNQFGNKTNQQMSTNGQPSLPS | ||||||
Region | 307-341 | Disordered | ||||
Sequence: SQQSAMNQFGNKTNQQMSTNGQPSLPSYMDFTKRG |
Sequence similarities
Belongs to the bZIP family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length341
- Mass (Da)37,791
- Last updated2000-10-01 v1
- Checksum10BE8D8230C53531
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 19-33 | Basic and acidic residues | ||||
Sequence: MPEAPRQRISHHRRA | ||||||
Compositional bias | 75-95 | Polar residues | ||||
Sequence: QPQASPMSVDSEETSSNGVVP | ||||||
Sequence conflict | 132 | in Ref. 4; AAM63070 | ||||
Sequence: I → L | ||||||
Compositional bias | 307-333 | Polar residues | ||||
Sequence: SQQSAMNQFGNKTNQQMSTNGQPSLPS | ||||||
Sequence conflict | 319 | in Ref. 4; AAM63070 | ||||
Sequence: T → K |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC009526 EMBL· GenBank· DDBJ | AAF63120.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE31988.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY065453 EMBL· GenBank· DDBJ | AAL38894.1 EMBL· GenBank· DDBJ | mRNA | ||
AY117284 EMBL· GenBank· DDBJ | AAM51359.1 EMBL· GenBank· DDBJ | mRNA | ||
AY085857 EMBL· GenBank· DDBJ | AAM63070.1 EMBL· GenBank· DDBJ | mRNA | ||
AF225983 EMBL· GenBank· DDBJ | AAF37279.4 EMBL· GenBank· DDBJ | mRNA |