Q9M9S3 · WHY1_ARATH
- ProteinSingle-stranded DNA-binding protein WHY1, chloroplastic
- GeneWHY1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids263 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Single-stranded DNA-binding protein that functions in both chloroplasts and nucleus. In chloroplasts, maintains plastid genome stability by preventing break-induced and short homology-dependent illegitimate recombinations. In nucleus, modulates telomere length homeostasis by inhibiting the action of the telomerase at the extreme termini of chromosomes. Is recruited to a distal element upstream of the kinesin KP1 to mediate the transcriptional repression of KP1. Is required for full salicylic acid-dependent plant disease resistance responses. Can bind double-stranded DNA in vivo.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast nucleoid | |
Cellular Component | chromosome, telomeric region | |
Cellular Component | DNA repair complex | |
Cellular Component | extracellular region | |
Molecular Function | DNA binding | |
Molecular Function | mRNA binding | |
Molecular Function | single-stranded DNA binding | |
Molecular Function | telomeric DNA binding | |
Biological Process | defense response | |
Biological Process | DNA repair | |
Biological Process | negative regulation of telomere maintenance via telomerase | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | regulation of telomere maintenance | |
Biological Process | salicylic acid mediated signaling pathway | |
Biological Process | systemic acquired resistance |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameSingle-stranded DNA-binding protein WHY1, chloroplastic
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9M9S3
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Can localize to both chloroplast and nucleus (PubMed:15967440).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth conditions, but mutant plants present a progressive lengthening of telomeric repeats.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 91 | No effect on DNA binding. Affects its function in DNA repair. | ||||
Sequence: K → A | ||||||
Mutagenesis | 148 | In atwhy1.2; reduces binding activity to single-stranded DNA. | ||||
Sequence: G → E | ||||||
Mutagenesis | 183 | In atwhy1.1; reduces binding activity to single-stranded DNA. | ||||
Sequence: P → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 11 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-47 | Chloroplast | ||||
Sequence: MSQLLSTPLMAVNSNPRFLSSSSVLVTGGFAVKRHGFALKPTTKTVK | ||||||
Chain | PRO_0000420447 | 48-263 | Single-stranded DNA-binding protein WHY1, chloroplastic | |||
Sequence: LFSVKSRQTDYFEKQRFGDSSSSPSPAEGLPARFYVGHSIYKGKAALTVDPRAPEFVALDSGAFKLSKDGFLLLQFAPSAGVRQYDWSKKQVFSLSVTEIGTLVSLGPRESCEFFHDPFKGKSDEGKVRKVLKVEPLPDGSGHFFNLSVQNKLVNVDESIYIPITRAEFAVLISAFNFVLPYLIGWHAFANSIKPEETSRVNNASPNYGGDYEWNR |
Proteomic databases
Expression
Induction
By salicylic acid (SA) and infection by H.parasitica.
Gene expression databases
Interaction
Subunit
Homotetramer.
Complex viewer
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 89-94 | Required for ssDNA binding | ||||
Sequence: KGKAAL | ||||||
Motif | 167-180 | Nuclear localization signal | ||||
Sequence: KGKSDEGKVRKVLK |
Sequence similarities
Belongs to the Whirly family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length263
- Mass (Da)29,058
- Last updated2000-10-01 v1
- ChecksumE90F85763198C614
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC012188 EMBL· GenBank· DDBJ | AAF43941.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE29160.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF332452 EMBL· GenBank· DDBJ | AAG48815.1 EMBL· GenBank· DDBJ | mRNA | ||
AF370156 EMBL· GenBank· DDBJ | AAK43971.1 EMBL· GenBank· DDBJ | mRNA | ||
AY059097 EMBL· GenBank· DDBJ | AAL15203.1 EMBL· GenBank· DDBJ | mRNA |