Q9M879 · STAD5_ARATH
- ProteinStearoyl-[acyl-carrier-protein] 9-desaturase 5, chloroplastic
- GeneS-ACP-DES5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids396 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons 9 and 10 of the acyl chain.
Catalytic activity
- 2 H+ + O2 + octadecanoyl-[ACP] + 2 reduced [2Fe-2S]-[ferredoxin] = (9Z)-octadecenoyl-[ACP] + 2 H2O + 2 oxidized [2Fe-2S]-[ferredoxin]
2 CHEBI:15378 + CHEBI:15379 + RHEA-COMP:9656 + 2 RHEA-COMP:10001 = RHEA-COMP:9924 + 2 CHEBI:15377 + 2 RHEA-COMP:10000
Cofactor
Note: Binds 2 Fe2+ ions per subunit.
Pathway
Lipid metabolism; fatty acid metabolism.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 137 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 175 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 175 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 178 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 228 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 261 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 261 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 264 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast stroma | |
Molecular Function | acyl-[acyl-carrier-protein] desaturase activity | |
Molecular Function | metal ion binding | |
Molecular Function | stearoyl-[acp] desaturase activity | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | fatty acid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameStearoyl-[acyl-carrier-protein] 9-desaturase 5, chloroplastic
- EC number
- Short namesStearoyl-ACP desaturase 5
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9M879
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-29 | Chloroplast | ||||
Sequence: MAMAMDRIVFSPSSYVYRPCQARGSRSSR | ||||||
Chain | PRO_0000401423 | 30-396 | Stearoyl-[acyl-carrier-protein] 9-desaturase 5, chloroplastic | |||
Sequence: VSMASTIRSATTEVTNGRKLYIPPREVHVQVKHSMPPQKLEIFKSLEGWADETLLTYLKPVEKSWQPTDFLPEPESEGFYDQVKELRERCKELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTPWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPKTENNPYLGFIYTSFQERATFISHGNTARLAKDRGDLKLAQICGTIAADERRHETAYTKIVEKLFEIDPDGTILGLADMMKKKISMPAHLMYDGQDDNLFEHFSTVAQRLGVYTAKDYADILEFLVERWNVETLTDLSSEGHRAQDFVCGLPARIRKIEERAQGRAKEAAKNIPFSWIFGRNIRA |
Proteomic databases
PTM databases
Expression
Tissue specificity
Ubiquitously expressed with a preference in leaves, flowers and stems.
Induction
Positively regulated by LEC1.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length396
- Mass (Da)45,303
- Last updated2000-10-01 v1
- ChecksumFFDF05586ECB8CAD
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 18 | in Ref. 4; AAM64846 | ||||
Sequence: R → L | ||||||
Sequence conflict | 70 | in Ref. 3; AAM20635/AAM91283 | ||||
Sequence: E → D |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC021640 EMBL· GenBank· DDBJ | AAF32470.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE73841.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY099784 EMBL· GenBank· DDBJ | AAM20635.1 EMBL· GenBank· DDBJ | mRNA | ||
AY128883 EMBL· GenBank· DDBJ | AAM91283.1 EMBL· GenBank· DDBJ | mRNA | ||
AY087294 EMBL· GenBank· DDBJ | AAM64846.1 EMBL· GenBank· DDBJ | mRNA |