Q9M4B5 · PFD4_ARATH

Function

function

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it (PubMed:19825635).
Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins (PubMed:19825635).
Together with other chaperonins, contribute to the regulation of gene expression by modulating the spliceosome function on pre-mRNA splicing post-transcriptionally by acting as a co-chaperone of Hsp90 to control levels of LSM8, especially in cold conditions (PubMed:32396196).
Required for microtubules (MTs) (MTs) organization and dynamicity (PubMed:19004800, PubMed:28412546).
Involved in the process leading to microtubules dissociation in response to gibberellic acid (GA) probably due to the DELLA proteins-mediated translocation of the prefoldin co-chaperone complex from the cytoplasm to the nucleus (PubMed:28412546).
Prevents cold acclimation (e.g. 7 days at 4 degrees Celsius) in a DELLA proteins-dependent manner by promoting nuclear proteasome-mediated HY5 degradation, thus modulating the expression of several genes and reducing anthocyanin biosynthesis, but seems not involved in constitutive freezing tolerance (PubMed:28412546).

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular Componentprefoldin complex
Molecular FunctionHsp90 protein binding
Molecular Functionunfolded protein binding
Biological Processcellular response to gibberellin stimulus
Biological Processcold acclimation
Biological Processcortical microtubule organization
Biological ProcessmRNA processing
Biological Processprotein folding
Biological Processresponse to cold
Biological Processresponse to salt stress

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Prefoldin subunit 4
  • Alternative names
    • ABI3-interacting protein 3
    • Gene involved in microtubule biogenesis 3

Gene names

    • Name
      PFD4
    • Synonyms
      AIP3
      , GIM3
    • ORF names
      F22O13.27
    • Ordered locus names
      At1g08780

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Landsberg erecta
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9M4B5
  • Secondary accessions
    • Q84VV8

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Cytoplasm
Nucleus
Note: In the presence of gibberellic acid (GA) and at room temperature, the prefoldin complex stays in the cytoplasm and is functional (PubMed:28412546).
But in the absence of GA or in response to cold, the prefoldin complex is localized to the nucleus in the presence of DELLA proteins, which severely compromises alpha/beta-tubulin heterodimer availability, thus affecting microtubules (MTs) organization (PubMed:28412546).
This changing subcellular localization follows a daily rhythm coordinated oscillation (By similarity).

Keywords

Phenotypes & Variants

Disruption phenotype

Hypersensitivity to oryzalin (a microtubule inhibitor) (PubMed:19004800).
Increased sensitivity to high salt treatment (PubMed:32396196).
Smaller plants due to reduced cells size and associated with lower levels of both alpha and beta tubulin subunits, as well as highly disorganized cortical microtubules (MTs) (PubMed:28412546, PubMed:32396196).
Increased capacity to tolerate freezing temperatures upon acclimation (e.g. 7 days at 4 degrees Celsius) associated with a continuous accumulation of HY5 in cold conditions, characterized by a lower ubiquitination status (PubMed:28412546).
Reduced levels of LSM8 and of LSM2-8 complex via a post-transcriptional process, especially in cold conditions (at protein level) (PubMed:32396196).
Slightly reduced production of U6 snRNA at room temperature, but to higher extent in cold situation (PubMed:32396196).
Lower pre-mRNA splicing events and reduced production of U6 snRNA in plants lacking PFD2, PFD4 and PFD6, probably due to a reduced activity of the LSM2-8 complex (PubMed:32396196).

Variants

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The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001248451-129Prefoldin subunit 4

Proteomic databases

PTM databases

Expression

Induction

Accumulates in response to cold (PubMed:28412546, PubMed:32396196).
Reduced levels upon treatment with geldanamycin (GDA), a Hsp90 inhibitor (PubMed:32396196).

Gene expression databases

Interaction

Subunit

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits forming prefoldin co-chaperone complex (By similarity).
Interacts with ABI3 (via C-terminus) (PubMed:10743655).
Interacts with PFD6 (PubMed:19004800).
Binds to HY5 in the nucleus and at low temperature (e.g. at 4 degrees Celsius) (PubMed:28412546).
Interacts with LSM8, a specific subunit of the LSM2-8 complex, which is a core component of the spliceosome (PubMed:32396196).
Binds to HSP90 to facilitate the formation of a larger complex made at least of HSP90, PFD4 and LSM8 (PubMed:32396196).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY Q9M4B5ABI3 Q015932EBI-2130809, EBI-1578892
BINARY Q9M4B5MPK11 Q9LMM53EBI-2130809, EBI-2358699

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for coiled coil.

TypeIDPosition(s)Description
Coiled coil25-59
Coiled coil95-115

Sequence similarities

Belongs to the prefoldin subunit beta family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    129
  • Mass (Da)
    14,881
  • Last updated
    2011-05-31 v3
  • Checksum
    1562DF409E43884A
MQQQGSKSGSEMEVTWEDQQNINIFSRLNNRVHDLDDDIKSAKEKCENLEDAGNELILADEEMVRFQIGEVFAHVPRDDVETKIEEMKEATCKSLEKLEQEKESIVTQMAALKKVLYAKFKDSINLEED

Sequence caution

The sequence AAF99774.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB75510.1 differs from that shown. Reason: Miscellaneous discrepancy Intron retention.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC003981
EMBL· GenBank· DDBJ
AAF99774.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002684
EMBL· GenBank· DDBJ
AEE28346.1
EMBL· GenBank· DDBJ
Genomic DNA
BT004788
EMBL· GenBank· DDBJ
AAO44054.1
EMBL· GenBank· DDBJ
mRNA
AK227759
EMBL· GenBank· DDBJ
BAE99742.1
EMBL· GenBank· DDBJ
mRNA
AJ251088
EMBL· GenBank· DDBJ
CAB75510.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.

Genome annotation databases

Similar Proteins

Disclaimer

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