Q9M2U5 · CHI5_ARATH
- ProteinEndochitinase EP3
- GeneEP3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids273 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Probably involved in hypersensitive reaction upon Xanthomonas campestris infection.
Catalytic activity
pH Dependence
Optimum pH is 4.8.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 136 | Proton donor | ||||
Sequence: E |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | chitin binding | |
Molecular Function | chitinase activity | |
Biological Process | cell wall macromolecule catabolic process | |
Biological Process | chitin catabolic process | |
Biological Process | defense response | |
Biological Process | polysaccharide catabolic process | |
Biological Process | response to bacterium | |
Biological Process | response to wounding | |
Biological Process | somatic embryogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameEndochitinase EP3
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9M2U5
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
PTM/Processing
Features
Showing features for signal, glycosylation, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-28 | |||||
Sequence: MLTPTISKSISLVTILLVLQAFSNTTKA | ||||||
Glycosylation | 24 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Chain | PRO_0000433911 | 29-273 | Endochitinase EP3 | |||
Sequence: QNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPPPANGVSVAEIVTQEFFNGIISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYIEEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATDPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQLGVDPGNNLTC | ||||||
Disulfide bond | 31↔39 | |||||
Sequence: CGCSSELCC | ||||||
Disulfide bond | 33↔45 | |||||
Sequence: CSSELCCSQFGFC | ||||||
Disulfide bond | 38↔52 | |||||
Sequence: CCSQFGFCGNTSDYC | ||||||
Glycosylation | 47 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 56↔61 | |||||
Sequence: CQQGPC | ||||||
Glycosylation | 157 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 270 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in cells surrounding embryos, stems, seedlings, pollen, roots, shoots, inflorescence, flowers, siliques and leaves (PubMed:11525512, PubMed:19420714).
Present in seedpods and seed embryos, but not in roots, inflorescence stems, leaves and flowers (PubMed:9426222).
Present in seedpods and seed embryos, but not in roots, inflorescence stems, leaves and flowers (PubMed:9426222).
Induction
Accumulates rapidly and transiently in leaves after inoculation with Xanthomonas campestris (PubMed:9426222).
Slightly repressed by wounding (PubMed:19420714).
Slightly repressed by wounding (PubMed:19420714).
Developmental stage
Expressed during somatic embryogenesis in nursing cells surrounding the embryos but not in embryos. Accumulates in mature pollen and growing pollen tubes until they enter the receptive synergid, but not in endosperm and integuments. In adult plants, present in hydathodes, stipules, root epidermis and emerging root hairs.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 29-63 | Chitin-binding type-1 | ||||
Sequence: QNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFA | ||||||
Region | 70-273 | Catalytic | ||||
Sequence: VSVAEIVTQEFFNGIISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYIEEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATDPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQLGVDPGNNLTC |
Sequence similarities
Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length273
- Mass (Da)29,436
- Last updated2000-10-01 v1
- Checksum1DB0A002F4EF44EC
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 139 | in Ref. 1; CAA74930 | ||||
Sequence: H → RN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Y14590 EMBL· GenBank· DDBJ | CAA74930.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL132971 EMBL· GenBank· DDBJ | CAB81807.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE79228.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT010422 EMBL· GenBank· DDBJ | AAQ62423.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176488 EMBL· GenBank· DDBJ | BAD44251.1 EMBL· GenBank· DDBJ | mRNA | ||
Z26409 EMBL· GenBank· DDBJ | CAA81243.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |