Q9M2U5 · CHI5_ARATH

Function

function

Probably involved in hypersensitive reaction upon Xanthomonas campestris infection.

Catalytic activity

  • Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
    EC:3.2.1.14 (UniProtKB | ENZYME | Rhea)

pH Dependence

Optimum pH is 4.8.

Features

Showing features for active site.

127320406080100120140160180200220240260
TypeIDPosition(s)Description
Active site136Proton donor

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Functionchitin binding
Molecular Functionchitinase activity
Biological Processcell wall macromolecule catabolic process
Biological Processchitin catabolic process
Biological Processdefense response
Biological Processpolysaccharide catabolic process
Biological Processresponse to bacterium
Biological Processresponse to wounding
Biological Processsomatic embryogenesis

Keywords

Enzyme and pathway databases

Protein family/group databases

    • CBM18Carbohydrate-Binding Module Family 18
    • GH19Glycoside Hydrolase Family 19

Names & Taxonomy

Protein names

  • Recommended name
    Endochitinase EP3
  • EC number
  • Alternative names
    • Chitinase class IV
      (AtchitIV
      )
    • Protein HOMOLOG OF CARROT EP3-3 CHITINASE
      (AtEP3
      )

Gene names

    • Name
      EP3
    • Synonyms
      CHIV
    • ORF names
      T12E18.110
    • Ordered locus names
      At3g54420

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9M2U5
  • Secondary accessions
    • O23248
    • Q42085

Proteomes

Organism-specific databases

Genome annotation databases

PTM/Processing

Features

Showing features for signal, glycosylation, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-28
Glycosylation24N-linked (GlcNAc...) asparagine
ChainPRO_000043391129-273Endochitinase EP3
Disulfide bond31↔39
Disulfide bond33↔45
Disulfide bond38↔52
Glycosylation47N-linked (GlcNAc...) asparagine
Disulfide bond56↔61
Glycosylation157N-linked (GlcNAc...) asparagine
Glycosylation270N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in cells surrounding embryos, stems, seedlings, pollen, roots, shoots, inflorescence, flowers, siliques and leaves (PubMed:11525512, PubMed:19420714).
Present in seedpods and seed embryos, but not in roots, inflorescence stems, leaves and flowers (PubMed:9426222).

Induction

Accumulates rapidly and transiently in leaves after inoculation with Xanthomonas campestris (PubMed:9426222).
Slightly repressed by wounding (PubMed:19420714).

Developmental stage

Expressed during somatic embryogenesis in nursing cells surrounding the embryos but not in embryos. Accumulates in mature pollen and growing pollen tubes until they enter the receptive synergid, but not in endosperm and integuments. In adult plants, present in hydathodes, stipules, root epidermis and emerging root hairs.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain29-63Chitin-binding type-1
Region70-273Catalytic

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    273
  • Mass (Da)
    29,436
  • Last updated
    2000-10-01 v1
  • Checksum
    1DB0A002F4EF44EC
MLTPTISKSISLVTILLVLQAFSNTTKAQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPPPANGVSVAEIVTQEFFNGIISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYIEEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATDPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQLGVDPGNNLTC

Sequence caution

The sequence CAA81243.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict139in Ref. 1; CAA74930

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Y14590
EMBL· GenBank· DDBJ
CAA74930.1
EMBL· GenBank· DDBJ
Genomic DNA
AL132971
EMBL· GenBank· DDBJ
CAB81807.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE79228.1
EMBL· GenBank· DDBJ
Genomic DNA
BT010422
EMBL· GenBank· DDBJ
AAQ62423.1
EMBL· GenBank· DDBJ
mRNA
AK176488
EMBL· GenBank· DDBJ
BAD44251.1
EMBL· GenBank· DDBJ
mRNA
Z26409
EMBL· GenBank· DDBJ
CAA81243.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

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