Q9M1W4 · HMT2_ARATH

Function

function

Catalyzes methyl transfer from S-methylmethionine (SMM) to adenosyl-L-homocysteine (AdoMet). SMM degradation (by HMT-1, HMT-2 and HMT-3) and biosynthesis (by MMT1) constitute the SMM cycle in plants, which is probably required to achieve short term control of AdoMet level.

Miscellaneous

In contrast to HMT-1, it is not inhibited by methionine.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
50 μMS-methylmethionine
225 μM(S,S)-AdoMet

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site245Zn2+ (UniProtKB | ChEBI)
Binding site312Zn2+ (UniProtKB | ChEBI)
Binding site313Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionS-adenosylmethionine-homocysteine S-methyltransferase activity
Molecular FunctionS-methylmethionine-homocysteine S-methyltransferase activity
Molecular Functionzinc ion binding
Biological Processmethionine biosynthetic process
Biological Processmethylation
Biological ProcessS-methylmethionine cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Homocysteine S-methyltransferase 2
  • EC number
  • Alternative names
    • S-methylmethionine:homocysteine methyltransferase 2 (AtHMT-2; SMM:Hcy S-methyltransferase 2)

Gene names

    • Name
      HMT-2
    • ORF names
      F16M2.100
    • Ordered locus names
      At3g63250

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9M1W4
  • Secondary accessions
    • Q0WTD0
    • Q9SDL6

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001146121-333Homocysteine S-methyltransferase 2

Proteomic databases

Expression

Tissue specificity

Expressed predominantly in roots. Expressed in rosette leaves, cauline leaves and developing seeds.

Gene expression databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain8-327Hcy-binding

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q9M1W4-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    333
  • Mass (Da)
    36,451
  • Last updated
    2000-10-01 v1
  • Checksum
    CB44D8F3BAC15D85
MTGNSFNSMKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADIISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSLLGGCCRTTPTTIRAIHKRLVNRRSL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
F4J0X9F4J0X9_ARATHHMT2293

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict103in Ref. 1; AAF23822

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF219223
EMBL· GenBank· DDBJ
AAF23822.1
EMBL· GenBank· DDBJ
Genomic DNA
AL138648
EMBL· GenBank· DDBJ
CAB86426.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE80456.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
ANM63398.1
EMBL· GenBank· DDBJ
Genomic DNA
AF428402
EMBL· GenBank· DDBJ
AAL16170.1
EMBL· GenBank· DDBJ
mRNA
BT010165
EMBL· GenBank· DDBJ
AAQ22634.1
EMBL· GenBank· DDBJ
mRNA
AK227627
EMBL· GenBank· DDBJ
BAE99618.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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