Q9LYU8 · AK1_ARATH
- ProteinAspartokinase 1, chloroplastic
- GeneAK1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids569 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the first step of essential amino acids lysine, threonine, methionine and isoleucine synthesis via the aspartate-family pathway.
Miscellaneous
Only one ACT domain (ACT1) is implicated in effector binding.
Catalytic activity
- L-aspartate + ATP = 4-phospho-L-aspartate + ADP
Activity regulation
Inhibited by S-adenosyl-L-methionine (SAM) and lysine in a synergistic manner. No inhibition by threonine, leucine or SAM alone, and no activation or inhibition by alanine, cysteine, isoleucine, serine, valine, methionine, glutamine, asparagine, glutamic acid or arginine.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
1700 μM | ATP | |||||
2037 μM | aspartate |
K(cat) is 23.4/sec.
Pathway
Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.
Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 91 | ATP (UniProtKB | ChEBI) | |||
Binding site | 94 | ATP (UniProtKB | ChEBI) | |||
Binding site | 123 | ATP (UniProtKB | ChEBI) | |||
Binding site | 207 | substrate | |||
Binding site | 413 | L-lysine (UniProtKB | ChEBI); allosteric effector | |||
Binding site | 415 | L-lysine (UniProtKB | ChEBI); allosteric effector | |||
Binding site | 430 | S-adenosyl-L-methionine (UniProtKB | ChEBI); allosteric effector | |||
Binding site | 431 | L-lysine (UniProtKB | ChEBI); allosteric effector | |||
Binding site | 432 | L-lysine (UniProtKB | ChEBI); allosteric effector | |||
Binding site | 437 | L-lysine (UniProtKB | ChEBI); allosteric effector | |||
Binding site | 452 | S-adenosyl-L-methionine (UniProtKB | ChEBI); allosteric effector | |||
Binding site | 453 | S-adenosyl-L-methionine (UniProtKB | ChEBI); allosteric effector | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast stroma | |
Molecular Function | aspartate kinase activity | |
Molecular Function | ATP binding | |
Biological Process | amino acid biosynthetic process | |
Biological Process | lysine biosynthetic process via diaminopimelate | |
Biological Process | threonine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAspartokinase 1, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9LYU8
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-90 | Chloroplast | |||
Chain | PRO_0000248157 | 91-569 | Aspartokinase 1, chloroplastic | ||
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 405-483 | ACT 1 | |||
Domain | 484-560 | ACT 2 | |||
Sequence similarities
Belongs to the aspartokinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length569
- Mass (Da)62,298
- Last updated2000-10-01 v1
- MD5 Checksum7E065E6BBDD5C5196966C22496D613D7
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 369 | in Ref. 1; CAA67376 | |||
Sequence conflict | 569 | in Ref. 1; CAA67376 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X98873 EMBL· GenBank· DDBJ | CAA67376.1 EMBL· GenBank· DDBJ | mRNA | ||
AL163491 EMBL· GenBank· DDBJ | CAB86635.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED91874.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT000493 EMBL· GenBank· DDBJ | AAN18062.1 EMBL· GenBank· DDBJ | mRNA | ||
AY057674 EMBL· GenBank· DDBJ | AAL15305.1 EMBL· GenBank· DDBJ | mRNA |