Q9LYD9 · EMF1_ARATH
- ProteinProtein EMBRYONIC FLOWER 1
- GeneEMF1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1096 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription repressor that regulates phase transition during shoot, flower and seeds development. Controls leaves development, shoot architecture and flowering by delaying both the vegetative to reproductive transition and flower initiation. Participates in polycomb group (PcG) protein complex-mediated (including EMF2) silencing of the flower homeotic genes AGAMOUS (AG), PISTILLATA (PI), and APETALA3 (AP3), as well as of some regulatory genes such as ABSCISIC ACID INSENSITIVE3 (ABI3), LONG VEGETATIVE PHASE1 (LOV1), and FLOWERING LOCUS C (FLC) during vegetative development. Required for histone methylation or for maintaining a stable histone methylation (e.g. H3K27me3) pattern of repressed target genes (including genes involved in salt stress response and flower development); this repression is counteracted by ULT1 (PubMed:23632855).
Can bind non specifically DNA (both double- and single-stranded) and RNA
Can bind non specifically DNA (both double- and single-stranded) and RNA
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | double-stranded DNA binding | |
Molecular Function | RNA binding | |
Molecular Function | single-stranded DNA binding | |
Biological Process | epigenetic regulation of gene expression | |
Biological Process | hyperosmotic salinity response | |
Biological Process | meristem determinacy | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | negative regulation of flower development | |
Biological Process | negative regulation of gene expression, epigenetic | |
Biological Process | post-embryonic development | |
Biological Process | regulation of flower development | |
Biological Process | regulation of response to salt stress | |
Biological Process | shoot system development |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameProtein EMBRYONIC FLOWER 1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9LYD9
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Follow a speckle-like pattern.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Abolished rosette shoot development, reduced inflorescence with several flowers lacking petals, and differentiation of the apical meristem from indeterminate to determinate growth by producing a single terminal flower on all nodes. Altered inflorescence-to-flower transition. Degenerated flowers with only carpelloid structures capped with stigmatic papillae but lacking leaves, petals and stamen. Derepressed seed development program. Decreased H3K27me3 marks but increased H3K4me3 marks on target gene loci (PubMed:23632855).
Plants missing both EMF1 and ULT1 have rescued normal H3K27me3 marks and H3K4me3 marks (PubMed:23632855).
Plants missing both EMF1 and ULT1 have rescued normal H3K27me3 marks and H3K4me3 marks (PubMed:23632855).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 65 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000405409 | 1-1096 | Protein EMBRYONIC FLOWER 1 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in mature embryo, root tips, cotyledons, leaves, stems, shoot apex, and flower clusters, with highest levels in flowers. The presence in the shoot apical meristem (SAM) is required to maintain vegetative development and prevent early flowering.
Developmental stage
After flowering, expressed in the stigma and anthers.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 155-189 | Disordered | |||
Compositional bias | 168-182 | Polar residues | |||
Motif | 170-177 | Nuclear localization signal 1 | |||
Region | 274-296 | Disordered | |||
Motif | 281-288 | Nuclear localization signal 2 | |||
Compositional bias | 315-339 | Polar residues | |||
Region | 315-348 | Disordered | |||
Region | 337-617 | DNA-binding | |||
Region | 366-420 | Disordered | |||
Compositional bias | 384-400 | Polar residues | |||
Region | 563-612 | Disordered | |||
Compositional bias | 569-605 | Basic and acidic residues | |||
Region | 629-651 | Disordered | |||
Region | 866-1096 | DNA-binding | |||
Region | 1007-1032 | Disordered | |||
Compositional bias | 1018-1032 | Polar residues | |||
Region | 1070-1096 | Disordered | |||
Motif | 1071-1078 | Nuclear localization signal 3 | |||
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,096
- Mass (Da)121,671
- Last updated2000-10-01 v1
- Checksum190308C7A96A0F61
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 168-182 | Polar residues | |||
Compositional bias | 315-339 | Polar residues | |||
Compositional bias | 384-400 | Polar residues | |||
Compositional bias | 569-605 | Basic and acidic residues | |||
Compositional bias | 1018-1032 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF319968 EMBL· GenBank· DDBJ | AAK98528.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL163815 EMBL· GenBank· DDBJ | CAB87713.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED91692.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | ANM68488.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | ANM68489.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598316 EMBL· GenBank· DDBJ | ABR08848.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598317 EMBL· GenBank· DDBJ | ABR08849.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598318 EMBL· GenBank· DDBJ | ABR08850.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598319 EMBL· GenBank· DDBJ | ABR08851.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598320 EMBL· GenBank· DDBJ | ABR08852.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598321 EMBL· GenBank· DDBJ | ABR08853.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598322 EMBL· GenBank· DDBJ | ABR08854.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598323 EMBL· GenBank· DDBJ | ABR08855.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598324 EMBL· GenBank· DDBJ | ABR08856.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598325 EMBL· GenBank· DDBJ | ABR08857.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598326 EMBL· GenBank· DDBJ | ABR08858.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598327 EMBL· GenBank· DDBJ | ABR08859.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598328 EMBL· GenBank· DDBJ | ABR08860.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598329 EMBL· GenBank· DDBJ | ABR08861.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598330 EMBL· GenBank· DDBJ | ABR08862.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598331 EMBL· GenBank· DDBJ | ABR08863.1 EMBL· GenBank· DDBJ | Genomic DNA |