Q9LW27 · C71BF_ARATH

Function

function

Multifunctional enzyme involved in the biosynthesis of the indole-derived phytoalexin camalexin. Catalyzes two reactions, the formation of dihydrocamalexate from indole-3-acetonitrile-cysteine conjugate and the oxidative decarboxylation of dihydrocamalexate which is the final step in camalexin biosynthesis. Required for the resistance to the fungal pathogens A.brassicicola, B.cinerea, B.elliptica, B.tulipae, L.maculans and Colletotrichum higginsianum. Seems not to be required for resistance to P.syringae, P.porri, and not involved in age-related resistance.

Catalytic activity

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentintracellular membrane-bounded organelle
Cellular Componentmembrane
Molecular Functiondihydrocamalexic acid decarboxylase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Biological Processcamalexin biosynthetic process
Biological Processdefense response
Biological Processdefense response to fungus
Biological Processindole phytoalexin biosynthetic process
Biological Processregulation of systemic acquired resistance
Biological Processresponse to abscisic acid
Biological Processresponse to bacterium
Biological Processresponse to insect
Biological Processresponse to water deprivation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional dihydrocamalexate synthase/camalexin synthase
  • EC number
  • Alternative names
    • Cytochrome P450 71B15
    • Dihydrocamalexate:NADP(+) oxidoreductase (decarboxylating)
    • Protein PHYTOALEXIN DEFICIENT 3

Gene names

    • Name
      CYP71B15
    • Synonyms
      PAD3
    • ORF names
      MDJ14.15
    • Ordered locus names
      At3g26830

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9LW27
  • Secondary accessions
    • Q541X4

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1-21Helical

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype under normal growth conditions, but deficient in the phytoalexin camalexin accumulation upon bacterial infection and markedly increased susceptibility to infection by the necrotrophic fungus Alternaria brassicicola.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis176In pad3-2.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 26 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000520931-490Bifunctional dihydrocamalexate synthase/camalexin synthase

Proteomic databases

Expression

Induction

By salicylic acid, flagellin, oligogalacturonides, silver nitrate, UV-C and infection with A.alternata and various strains of P.syringae. Activated by the transcription factor WRKY33 upon infection with P.syringae.

Gene expression databases

Interaction

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY Q9LW27CYP71A12 O493405EBI-16915951, EBI-30855500
BINARY Q9LW27CYP71A13 O493425EBI-16915951, EBI-2356153

Complex viewer

View interactors in UniProtKB
View CPX-2833 in Complex Portal

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    490
  • Mass (Da)
    56,061
  • Last updated
    2000-10-01 v1
  • Checksum
    B8F84D85F252668D
MSVFLCFLVLLPLILIFLNVLKPSKYKLPPGPKKLPIIGNLHQRRTLHPRNRRNLAEMYGPVALLQYGFVPVVAISSKEAAEEVLKINDLECCSRPEAAGMRATFYNFKDIGMAPFGDEWSLMRKLSVVELFSVKKLQSFKYIIEEENNLCVKKLSEFATRQSPVNLERAIFTLVGNIVCRIGYGINLYECDFFEADRVVDLVLKAEAVIRETVFSDFFPGRIGRFIDCISGQNRRLKNNFSVVDTFFQNVLNEHLKPGRESSTIVDLMIDMKKKQENDGDALKFTTDHLKGMISDIFVAGIGGVAGITLWGMTELIRNPRVMKKVQDEIRTTLGDKKERIKEEDLNQLHYFKLVVKETLRLHPTTPLLLPRQTMSHIKIQGYDVPAKTQILVNVYAMGRDPKLWENADEFNPDRFLDSSVDFKGKNYEFIPFGSGRRICPGMTMGTILVEMALLNLLYFFDWGLAKQEEAKEIINGEENFLAFFQVLHH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB016889
EMBL· GenBank· DDBJ
BAB01230.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE77220.1
EMBL· GenBank· DDBJ
Genomic DNA
AK117967
EMBL· GenBank· DDBJ
BAC42604.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help