Q9LW27 · C71BF_ARATH
- ProteinBifunctional dihydrocamalexate synthase/camalexin synthase
- GeneCYP71B15
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids490 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Multifunctional enzyme involved in the biosynthesis of the indole-derived phytoalexin camalexin. Catalyzes two reactions, the formation of dihydrocamalexate from indole-3-acetonitrile-cysteine conjugate and the oxidative decarboxylation of dihydrocamalexate which is the final step in camalexin biosynthesis. Required for the resistance to the fungal pathogens A.brassicicola, B.cinerea, B.elliptica, B.tulipae, L.maculans and Colletotrichum higginsianum. Seems not to be required for resistance to P.syringae, P.porri, and not involved in age-related resistance.
Catalytic activity
- 2-(L-cystein-S-yl)-2-(1H-indol-3-yl)-acetonitrile + 2 reduced [NADPH--hemoprotein reductase] + 2 O2 = camalexin + hydrogen cyanide + 2 oxidized [NADPH--hemoprotein reductase] + CO2 + 4 H2O + 2 H+This reaction proceeds in the forward direction.
CHEBI:79191 + 2 RHEA-COMP:11964 + 2 CHEBI:15379 = CHEBI:22990 + CHEBI:18407 + 2 RHEA-COMP:11965 + CHEBI:16526 + 4 CHEBI:15377 + 2 CHEBI:15378 - 2-(L-cystein-S-yl)-2-(1H-indol-3-yl)-acetonitrile + reduced [NADPH--hemoprotein reductase] + O2 = (R)-dihydrocamalexate + hydrogen cyanide + oxidized [NADPH--hemoprotein reductase] + 2 H2O + 2 H+This reaction proceeds in the forward direction.
- (R)-dihydrocamalexate + reduced [NADPH--hemoprotein reductase] + O2 = camalexin + oxidized [NADPH--hemoprotein reductase] + CO2 + 2 H2OThis reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | membrane | |
Molecular Function | dihydrocamalexic acid decarboxylase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | camalexin biosynthetic process | |
Biological Process | defense response | |
Biological Process | defense response to fungus | |
Biological Process | indole phytoalexin biosynthetic process | |
Biological Process | regulation of systemic acquired resistance | |
Biological Process | response to abscisic acid | |
Biological Process | response to bacterium | |
Biological Process | response to insect | |
Biological Process | response to water deprivation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional dihydrocamalexate synthase/camalexin synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9LW27
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 1-21 | Helical | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth conditions, but deficient in the phytoalexin camalexin accumulation upon bacterial infection and markedly increased susceptibility to infection by the necrotrophic fungus Alternaria brassicicola.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 176 | In pad3-2. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 26 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000052093 | 1-490 | Bifunctional dihydrocamalexate synthase/camalexin synthase | ||
Proteomic databases
Expression
Induction
By salicylic acid, flagellin, oligogalacturonides, silver nitrate, UV-C and infection with A.alternata and various strains of P.syringae. Activated by the transcription factor WRKY33 upon infection with P.syringae.
Gene expression databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q9LW27 | CYP71A12 O49340 | 5 | EBI-16915951, EBI-30855500 | |
BINARY | Q9LW27 | CYP71A13 O49342 | 5 | EBI-16915951, EBI-2356153 |
Complex viewer
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length490
- Mass (Da)56,061
- Last updated2000-10-01 v1
- ChecksumB8F84D85F252668D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB016889 EMBL· GenBank· DDBJ | BAB01230.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE77220.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK117967 EMBL· GenBank· DDBJ | BAC42604.1 EMBL· GenBank· DDBJ | mRNA |