Q9LVQ0 · PME31_ARATH

Function

function

Acts in the modification of cell walls via demethylesterification of cell wall pectin (PubMed:18936961).
Acts in a blockwise manner, resulting in a cell wall rigidification

Miscellaneous

This is the only member of the pectinesterase family that do not contain a transmembrane or a signal peptide.

Catalytic activity

Activity regulation

Does not require salt for activity. Not inhibited by kiwi pectin methylesterase inhibitor (PMEI).

pH Dependence

Optimum pH is 8.0-8.5.

Temperature Dependence

Fully active at 0 or 30 degrees Celsius, inactive at 55 degrees Celsius.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site91substrate
Binding site121substrate
Site143Transition state stabilizer
Active site144Proton donor
Active site165Nucleophile
Binding site222substrate
Binding site224substrate

GO annotations

AspectTerm
Molecular Functionpectinesterase activity
Biological Processcell wall modification
Biological Processdefense response to Gram-negative bacterium
Biological Processpectin catabolic process
Biological Processpectin metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pectinesterase 31
  • EC number
  • Short names
    PE 31
  • Alternative names
    • Pectin methylesterase 31 (AtPME31)

Gene names

    • Name
      PME31
    • Synonyms
      ARATH31
    • ORF names
      MXE2.5
    • Ordered locus names
      At3g29090

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9LVQ0

Proteomes

Organism-specific databases

Genome annotation databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003716831-317Pectinesterase 31

Proteomic databases

Expression

Tissue specificity

Expressed in siliques.

Developmental stage

Expressed during late developmental phases of siliques.

Gene expression databases

Interaction

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q9LVQ0RFC4 Q93ZX14EBI-4466887, EBI-4470690

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the pectinesterase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    317
  • Mass (Da)
    35,520
  • Last updated
    2000-10-01 v1
  • Checksum
    BCD96098853B585B
MATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAGISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHIHCKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMDACIRNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQDRPWLCLRMGVKTPYSA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB018121
EMBL· GenBank· DDBJ
BAB01985.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE77534.1
EMBL· GenBank· DDBJ
Genomic DNA
AY070091
EMBL· GenBank· DDBJ
AAL49785.1
EMBL· GenBank· DDBJ
mRNA
AY096561
EMBL· GenBank· DDBJ
AAM20211.1
EMBL· GenBank· DDBJ
mRNA
AY084418
EMBL· GenBank· DDBJ
AAM60992.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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