Q9LV40 · ROGF8_ARATH

Function

function

Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Active as homodimer.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentplasma membrane
Molecular Functionguanyl-nucleotide exchange factor activity
Molecular Functionidentical protein binding

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Rho guanine nucleotide exchange factor 8
  • Short names
    AtRopGEF8
  • Alternative names
    • Rho of plants guanine nucleotide exchange factor 8

Gene names

    • Name
      ROPGEF8
    • ORF names
      MOB24.21
    • Ordered locus names
      At3g24620

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9LV40
  • Secondary accessions
    • Q1PEM4

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Cell membrane
Note: Localizes to the apical region of the pollen tube plasma membrane.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis98Loss of homodimerization and GEF activity.
Mutagenesis161Decreases GEF activity 25-fold; when associated with A-175.
Mutagenesis175Decreases GEF activity 25-fold; when associated with A-161.
Mutagenesis234Decreases GEF activity 14-fold.
Mutagenesis235Loss of GEF activity.
Mutagenesis413Decreases GEF activity 20-fold.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 62 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004066081-523Rho guanine nucleotide exchange factor 8

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in pollen grains and pollen tubes.

Gene expression databases

Interaction

Subunit

Homodimer. The homodimer interacts with ARAC5/ROP4. Interacts with ARAC11/ROP1 and ARAC10/ROP11. Interacts with PRK6 (PubMed:26961657).

Protein-protein interaction databases

Family & Domains

Features

Showing features for compositional bias, region, domain.

Type
IDPosition(s)Description
Compositional bias44-67Polar residues
Region44-83Disordered
Domain76-440PRONE
Compositional bias442-461Polar residues
Region442-523Disordered
Compositional bias479-501Polar residues

Domain

The PRONE (plant-specific Rop nucleotide exchanger) domain is responsible for the GEF activity.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    523
  • Mass (Da)
    58,741
  • Last updated
    2000-10-01 v1
  • Checksum
    F84808EC9F70811F
MVAALERGLSASKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFVESPVESSLPMISPLTRPGKRSERQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCTEIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKWWLPPVKVPPGGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGRASLGDSIYKSITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSWGSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILESLAYTVMSRIEDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSKTLLDFMGWNDNSSKGGDKPTKSPNLTPKKLSYLEKLENLNGFRSPKDRH

Features

Showing features for compositional bias, sequence conflict.

Type
IDPosition(s)Description
Compositional bias44-67Polar residues
Sequence conflict278in Ref. 3; ABE65963
Compositional bias442-461Polar residues
Compositional bias479-501Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB020746
EMBL· GenBank· DDBJ
BAB02015.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE76930.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ446693
EMBL· GenBank· DDBJ
ABE65963.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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