Q9LV40 · ROGF8_ARATH
- ProteinRho guanine nucleotide exchange factor 8
- GeneROPGEF8
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids523 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Active as homodimer.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | guanyl-nucleotide exchange factor activity | |
Molecular Function | identical protein binding |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameRho guanine nucleotide exchange factor 8
- Short namesAtRopGEF8
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9LV40
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to the apical region of the pollen tube plasma membrane.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 98 | Loss of homodimerization and GEF activity. | |||
Mutagenesis | 161 | Decreases GEF activity 25-fold; when associated with A-175. | |||
Mutagenesis | 175 | Decreases GEF activity 25-fold; when associated with A-161. | |||
Mutagenesis | 234 | Decreases GEF activity 14-fold. | |||
Mutagenesis | 235 | Loss of GEF activity. | |||
Mutagenesis | 413 | Decreases GEF activity 20-fold. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 62 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000406608 | 1-523 | Rho guanine nucleotide exchange factor 8 | ||
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in pollen grains and pollen tubes.
Gene expression databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 44-67 | Polar residues | |||
Region | 44-83 | Disordered | |||
Domain | 76-440 | PRONE | |||
Compositional bias | 442-461 | Polar residues | |||
Region | 442-523 | Disordered | |||
Compositional bias | 479-501 | Polar residues | |||
Domain
The PRONE (plant-specific Rop nucleotide exchanger) domain is responsible for the GEF activity.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length523
- Mass (Da)58,741
- Last updated2000-10-01 v1
- ChecksumF84808EC9F70811F
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 44-67 | Polar residues | |||
Sequence conflict | 278 | in Ref. 3; ABE65963 | |||
Compositional bias | 442-461 | Polar residues | |||
Compositional bias | 479-501 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB020746 EMBL· GenBank· DDBJ | BAB02015.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE76930.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ446693 EMBL· GenBank· DDBJ | ABE65963.1 EMBL· GenBank· DDBJ | mRNA |