Q9LSP7 · UBC32_ARATH

Function

function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

Catalytic activity

  • S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.
    EC:2.3.2.23 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for active site.

130920406080100120140160180200220240260280300
TypeIDPosition(s)Description
Active site93Glycyl thioester intermediate

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentmembrane
Cellular Componentperinuclear region of cytoplasm
Molecular FunctionATP binding
Molecular Functionubiquitin conjugating enzyme activity
Molecular Functionubiquitin-protein transferase activity
Biological Processcellular response to water deprivation
Biological Processnegative regulation of stomatal opening
Biological Processprotein ubiquitination
Biological Processubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • 3.A.16.1.5the endoplasmic reticular retrotranslocon (er-rt) family

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin-conjugating enzyme E2 32
  • EC number
  • Alternative names
    • E2 ubiquitin-conjugating enzyme 32
    • Ubiquitin carrier protein 32

Gene names

    • Name
      UBC32
    • ORF names
      K14A17.7
    • Ordered locus names
      At3g17000

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9LSP7
  • Secondary accessions
    • Q4TYX9

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane275-295Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003451971-309Ubiquitin-conjugating enzyme E2 32

Proteomic databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain11-166UBC core

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    309
  • Mass (Da)
    34,322
  • Last updated
    2000-10-01 v1
  • Checksum
    B4C77186E1294D51
MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIYHGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAFMPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPLPLECSQAPSIVSEAHSQVEPQEAITVVEERSIATTDTIVDDQIIEETAEAVNTAASVVPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKSNGYSTGFMDDQS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB026636
EMBL· GenBank· DDBJ
BAA94978.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE75893.1
EMBL· GenBank· DDBJ
Genomic DNA
AF386935
EMBL· GenBank· DDBJ
AAK62380.1
EMBL· GenBank· DDBJ
mRNA
BT020530
EMBL· GenBank· DDBJ
AAW52556.1
EMBL· GenBank· DDBJ
mRNA
DQ027045
EMBL· GenBank· DDBJ
AAY44871.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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