Q9LSE2 · ICE1_ARATH
- ProteinTranscription factor ICE1
- GeneSCRM
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids494 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. Functions as a dimer with SPCH during stomatal initiation (PubMed:18641265, PubMed:28507175).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | cis-regulatory region sequence-specific DNA binding | |
Molecular Function | core promoter sequence-specific DNA binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | protein dimerization activity | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | abscisic acid-activated signaling pathway | |
Biological Process | endosperm development | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | positive regulation of DNA-templated transcription | |
Biological Process | regulation of abscisic acid biosynthetic process | |
Biological Process | response to cold | |
Biological Process | response to freezing | |
Biological Process | seed dormancy process | |
Biological Process | seed morphogenesis | |
Biological Process | stomatal lineage progression |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameTranscription factor ICE1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9LSE2
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
The ice1-2 scrm2-1 double mutant lacks stomata so that the epidermis only contains pavement cells.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 236 | In scrm-D and ice1-1D; cold resistance and excessive stomatal differentiation. Suppresses the cold-induction of CBF3/DREB1A. | |||
Mutagenesis | 312 | Loss of excessive stomatal differentiation; when associated with H-236. | |||
Mutagenesis | 393 | Loss of sumoylation. Increases freezing sensitivity. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 23 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, cross-link.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000127235 | 1-494 | Transcription factor ICE1 | ||
Cross-link | 393 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | |||
Post-translational modification
Ubiquitinated. Cold-treatment induced association with the E3 ubiquitin ligase HOS1 targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway. Sumoylated at Lys-393 by SIZ1. Sumoylated ICE1 represses HOS1 and MYB15 and facilitates the positive regulation of CBF3/DREB1A-dependent cold signaling and freezing tolerance.
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Widely expressed in the whole plant with high expression in leaves and stem. Broad expression within stomatal cell lineages of leaf epidermis.
Induction
By high-salt stress, cold stress and abscisic acid (ABA) treatment.
Gene expression databases
Interaction
Subunit
Homodimer (Probable). Efficient DNA binding requires dimerization with another bHLH protein (By similarity).
Interacts with the C-terminal part of HOS1 (PubMed:18641265).
Heterodimers with SPCH, MUTE, and FAMA (PubMed:18641265, PubMed:28507175).
Interacts with the C-terminal part of HOS1 (PubMed:18641265).
Heterodimers with SPCH, MUTE, and FAMA (PubMed:18641265, PubMed:28507175).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q9LSE2 | HOS1 Q84JU6 | 3 | EBI-15583266, EBI-15583242 | |
BINARY | Q9LSE2 | LFR Q9LS90 | 3 | EBI-15583266, EBI-40270350 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-58 | Disordered | |||
Compositional bias | 30-58 | Polar residues | |||
Compositional bias | 240-255 | Polar residues | |||
Region | 240-264 | Disordered | |||
Region | 281-306 | Disordered | |||
Domain | 303-352 | bHLH | |||
Coiled coil | 344-365 | ||||
Region | 363-384 | Disordered | |||
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9LSE2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length494
- Mass (Da)53,539
- Last updated2000-10-01 v1
- MD5 ChecksumEBF6FAB44F3301780827D48023484084
Q9LSE2-2
- Name2
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1I9LRH4 | A0A1I9LRH4_ARATH | ICE1 | 476 |
Features
Showing features for compositional bias, sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 30-58 | Polar residues | |||
Sequence conflict | 93 | in Ref. 6; BAF00372 | |||
Sequence conflict | 190 | in Ref. 6; BAF00372 | |||
Compositional bias | 240-255 | Polar residues | |||
Alternative sequence | VSP_020190 | 336-363 | in isoform 2 | ||
Alternative sequence | VSP_020191 | 364-494 | in isoform 2 | ||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY195621 EMBL· GenBank· DDBJ | AAP14668.1 EMBL· GenBank· DDBJ | mRNA | ||
AB026648 EMBL· GenBank· DDBJ | BAB01738.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB016889 EMBL· GenBank· DDBJ | BAB01738.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE77208.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE77209.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE77210.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | ANM65181.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY079016 EMBL· GenBank· DDBJ | AAL84972.1 EMBL· GenBank· DDBJ | mRNA | ||
BT020379 EMBL· GenBank· DDBJ | AAV85734.1 EMBL· GenBank· DDBJ | mRNA | ||
AK228443 EMBL· GenBank· DDBJ | BAF00372.1 EMBL· GenBank· DDBJ | mRNA |