Q9LL41 · PCBER_PINTA
- ProteinPhenylcoumaran benzylic ether reductase PT1
- GenePCBER
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids308 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Oxidoreductase involved in lignan biosynthesis. Catalyzes the NADPH-dependent reduction of phenylcoumaran benzylic ethers. Converts dehydrodiconiferyl alcohol (DDC) to isodihydrodehydrodiconiferyl alcohol (IDDDC), and dihydrodehydrodiconiferyl alcohol (DDDC) to tetrahydrodehydrodiconiferyl alcohol (TDDC).
Catalytic activity
- --dehydrodiconiferyl alcohol + H+ + NADPH = (S)-isodihydrodehydrodiconiferyl alcohol + NADP+
- +-dehydrodiconiferyl alcohol + H+ + NADPH = (R)-isodihydrodehydrodiconiferyl alcohol + NADP+
- (2R,3S)-dihydrodehydrodiconiferyl alcohol + H+ + NADPH = (S)-tetrahydrodehydrodiconiferyl alcohol + NADP+
- (2S,3R)-dihydrodehydrodiconiferyl alcohol + H+ + NADPH = (R)-tetrahydrodehydrodiconiferyl alcohol + NADP+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.61 mM | dehydrodiconiferyl alcohol | |||||
1.95 mM | dihydrodehydrodiconiferyl alcohol |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
104.2 nmol/h/mg | toward dehydrodiconiferyl alcohol | ||||
55.8 nmol/h/mg | toward dihydrodehydrodiconiferyl alcohol |
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor | |
Biological Process | lignan biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhenylcoumaran benzylic ether reductase PT1
- EC number
- Short namesPCBER-Pt1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Pinopsida > Pinidae > Conifers I > Pinales > Pinaceae > Pinus > Pinus subgen. Pinus
Accessions
- Primary accessionQ9LL41
- Secondary accessions
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000442613 | 1-308 | Phenylcoumaran benzylic ether reductase PT1 | |||
Sequence: MGSRSRILLIGATGYIGRHVAKASLDLGHPTFLLVRESTASSNSEKAQLLESFKASGANIVHGSIDDHASLVEAVKNVDVVISTVGSLQIESQVNIIKAIKEVGTVKRFFPSEFGNDVDNVHAVEPAKSVFEVKAKVRRAIEAEGIPYTYVSSNCFAGYFLRSLAQAGLTAPPRDKVVILGDGNARVVFVKEEDIGTFTIKAVDDPRTLNKTLYLRLPANTLSLNELVALWEKKIDKTLEKAYVPEEEVLKLIADTPFPANISIAISHSIFVKGDQTNFEIGPAGVEASQLYPDVKYTTVDEYLSNFV |
Structure
Family & Domains
Sequence similarities
Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length308
- Mass (Da)33,524
- Last updated2000-10-01 v1
- ChecksumE93FA4456C7854BF
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 103 | in Ref. 1; AAC32591 | ||||
Sequence: V → I | ||||||
Sequence conflict | 129 | in Ref. 1; AAC32591 | ||||
Sequence: S → N |
Keywords
- Technical term