Q9LL41 · PCBER_PINTA

  • Protein
    Phenylcoumaran benzylic ether reductase PT1
  • Gene
    PCBER
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Oxidoreductase involved in lignan biosynthesis. Catalyzes the NADPH-dependent reduction of phenylcoumaran benzylic ethers. Converts dehydrodiconiferyl alcohol (DDC) to isodihydrodehydrodiconiferyl alcohol (IDDDC), and dihydrodehydrodiconiferyl alcohol (DDDC) to tetrahydrodehydrodiconiferyl alcohol (TDDC).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.61 mMdehydrodiconiferyl alcohol
1.95 mMdihydrodehydrodiconiferyl alcohol
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
104.2 nmol/h/mgtoward dehydrodiconiferyl alcohol
55.8 nmol/h/mgtoward dihydrodehydrodiconiferyl alcohol

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site11-17NADP+ (UniProtKB | ChEBI)
Binding site36NADP+ (UniProtKB | ChEBI)
Binding site46NADP+ (UniProtKB | ChEBI)
Active site134Proton acceptor
Binding site138NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionoxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
Biological Processlignan biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phenylcoumaran benzylic ether reductase PT1
  • EC number
  • Short names
    PCBER-Pt1
  • Alternative names
    • PtPCBER

Gene names

    • Name
      PCBER

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Pinopsida > Pinidae > Conifers I > Pinales > Pinaceae > Pinus > Pinus subgen. Pinus

Accessions

  • Primary accession
    Q9LL41
  • Secondary accessions
    • O81651

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004426131-308Phenylcoumaran benzylic ether reductase PT1

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    308
  • Mass (Da)
    33,524
  • Last updated
    2000-10-01 v1
  • Checksum
    E93FA4456C7854BF
MGSRSRILLIGATGYIGRHVAKASLDLGHPTFLLVRESTASSNSEKAQLLESFKASGANIVHGSIDDHASLVEAVKNVDVVISTVGSLQIESQVNIIKAIKEVGTVKRFFPSEFGNDVDNVHAVEPAKSVFEVKAKVRRAIEAEGIPYTYVSSNCFAGYFLRSLAQAGLTAPPRDKVVILGDGNARVVFVKEEDIGTFTIKAVDDPRTLNKTLYLRLPANTLSLNELVALWEKKIDKTLEKAYVPEEEVLKLIADTPFPANISIAISHSIFVKGDQTNFEIGPAGVEASQLYPDVKYTTVDEYLSNFV

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict103in Ref. 1; AAC32591
Sequence conflict129in Ref. 1; AAC32591

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF081678
EMBL· GenBank· DDBJ
AAC32591.1
EMBL· GenBank· DDBJ
mRNA
AF242490
EMBL· GenBank· DDBJ
AAF64173.2
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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