Q9LKF9 · CPSF2_ARATH

Function

function

CPSF plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A)polymerase and other factors to bring about cleavage and poly(A) addition (By similarity).
Required for antisense-RNA-mediated gene silencing (PubMed:17008405).

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular ComponentmRNA cleavage and polyadenylation specificity factor complex
Cellular Componentnucleus
Cellular Componentplasmodesma
Molecular FunctionRNA binding
Biological ProcessmRNA 3'-end processing by stem-loop binding and cleavage
Biological Processregulatory ncRNA-mediated post-transcriptional gene silencing

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Cleavage and polyadenylation specificity factor subunit 2
  • Alternative names
    • Cleavage and polyadenylation specificity factor 100 kDa subunit (AtCPSF100; CPSF 100 kDa subunit)
    • Protein EMBRYO DEFECTIVE 1265
    • Protein ENHANCED SILENCING PHENOTYPE 5

Gene names

    • Name
      CPSF100
    • Synonyms
      EMB1265, ESP5
    • ORF names
      MRO11.8
    • Ordered locus names
      At5g23880

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9LKF9
  • Secondary accessions
    • Q9FF92

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Nucleus
Cytoplasm
Note: Localized in the cytoplasm when associated with CPSF30.

Keywords

Phenotypes & Variants

Disruption phenotype

Impaired antisense-RNA-mediated gene silencing. Early flowering.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 44 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000743981-739Cleavage and polyadenylation specificity factor subunit 2

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Component of the CPSF complex, at least composed of CPSF160, CPSF100, CPSF73-I, CPSF73-II, CPSF30, FY and FIPS5. Forms a complex with cleavage and polyadenylation specificity factor (CPSF) subunits FY, PAPS2, CSTF50, CPSF30, CPSF73-I, CPSF73-II and CPSF160.

Binary interactions

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region411-430Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    739
  • Mass (Da)
    82,139
  • Last updated
    2001-10-18 v2
  • Checksum
    9ABFC916712AD464
MGTSVQVTPLCGVYNENPLSYLVSIDGFNFLIDCGWNDLFDTSLLEPLSRVASTIDAVLLSHPDTLHIGALPYAMKQLGLSAPVYATEPVHRLGLLTMYDQFLSRKQVSDFDLFTLDDIDSAFQNVIRLTYSQNYHLSGKGEGIVIAPHVAGHMLGGSIWRITKDGEDVIYAVDYNHRKERHLNGTVLQSFVRPAVLITDAYHALYTNQTARQQRDKEFLDTISKHLEVGGNVLLPVDTAGRVLELLLILEQHWSQRGFSFPIYFLTYVSSSTIDYVKSFLEWMSDSISKSFETSRDNAFLLRHVTLLINKTDLDNAPPGPKVVLASMASLEAGFAREIFVEWANDPRNLVLFTETGQFGTLARMLQSAPPPKFVKVTMSKRVPLAGEELIAYEEEQNRLKREEALRASLVKEEETKASHGSDDNSSEPMIIDTKTTHDVIGSHGPAYKDILIDGFVPPSSSVAPMFPYYDNTSEWDDFGEIINPDDYVIKDEDMDRGAMHNGGDVDGRLDEATASLMLDTRPSKVMSNELIVTVSCSLVKMDYEGRSDGRSIKSMIAHVSPLKLVLVHAIAEATEHLKQHCLNNICPHVYAPQIEETVDVTSDLCAYKVQLSEKLMSNVIFKKLGDSEVAWVDSEVGKTERDMRSLLPMPGAASPHKPVLVGDLKIADFKQFLSSKGVQVEFAGGGALRCGEYVTLRKVGPTGQKGGASGPQQILIEGPLCEDYYKIRDYLYSQFYLL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict49in Ref. 1; AAF82809
Sequence conflict441in Ref. 1; AAF82809

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF283277
EMBL· GenBank· DDBJ
AAF82809.1
EMBL· GenBank· DDBJ
mRNA
AB005244
EMBL· GenBank· DDBJ
BAB10061.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED93228.1
EMBL· GenBank· DDBJ
Genomic DNA
AY034982
EMBL· GenBank· DDBJ
AAK59487.1
EMBL· GenBank· DDBJ
mRNA
BT004374
EMBL· GenBank· DDBJ
AAO42368.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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