Q9LKF9 · CPSF2_ARATH
- ProteinCleavage and polyadenylation specificity factor subunit 2
- GeneCPSF100
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids739 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
CPSF plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A)polymerase and other factors to bring about cleavage and poly(A) addition (By similarity).
Required for antisense-RNA-mediated gene silencing (PubMed:17008405).
Required for antisense-RNA-mediated gene silencing (PubMed:17008405).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | mRNA cleavage and polyadenylation specificity factor complex | |
Cellular Component | nucleus | |
Cellular Component | plasmodesma | |
Molecular Function | RNA binding | |
Biological Process | mRNA 3'-end processing by stem-loop binding and cleavage | |
Biological Process | regulatory ncRNA-mediated post-transcriptional gene silencing |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameCleavage and polyadenylation specificity factor subunit 2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9LKF9
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
Impaired antisense-RNA-mediated gene silencing. Early flowering.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 44 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000074398 | 1-739 | Cleavage and polyadenylation specificity factor subunit 2 | |||
Sequence: MGTSVQVTPLCGVYNENPLSYLVSIDGFNFLIDCGWNDLFDTSLLEPLSRVASTIDAVLLSHPDTLHIGALPYAMKQLGLSAPVYATEPVHRLGLLTMYDQFLSRKQVSDFDLFTLDDIDSAFQNVIRLTYSQNYHLSGKGEGIVIAPHVAGHMLGGSIWRITKDGEDVIYAVDYNHRKERHLNGTVLQSFVRPAVLITDAYHALYTNQTARQQRDKEFLDTISKHLEVGGNVLLPVDTAGRVLELLLILEQHWSQRGFSFPIYFLTYVSSSTIDYVKSFLEWMSDSISKSFETSRDNAFLLRHVTLLINKTDLDNAPPGPKVVLASMASLEAGFAREIFVEWANDPRNLVLFTETGQFGTLARMLQSAPPPKFVKVTMSKRVPLAGEELIAYEEEQNRLKREEALRASLVKEEETKASHGSDDNSSEPMIIDTKTTHDVIGSHGPAYKDILIDGFVPPSSSVAPMFPYYDNTSEWDDFGEIINPDDYVIKDEDMDRGAMHNGGDVDGRLDEATASLMLDTRPSKVMSNELIVTVSCSLVKMDYEGRSDGRSIKSMIAHVSPLKLVLVHAIAEATEHLKQHCLNNICPHVYAPQIEETVDVTSDLCAYKVQLSEKLMSNVIFKKLGDSEVAWVDSEVGKTERDMRSLLPMPGAASPHKPVLVGDLKIADFKQFLSSKGVQVEFAGGGALRCGEYVTLRKVGPTGQKGGASGPQQILIEGPLCEDYYKIRDYLYSQFYLL |
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Component of the CPSF complex, at least composed of CPSF160, CPSF100, CPSF73-I, CPSF73-II, CPSF30, FY and FIPS5. Forms a complex with cleavage and polyadenylation specificity factor (CPSF) subunits FY, PAPS2, CSTF50, CPSF30, CPSF73-I, CPSF73-II and CPSF160.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9LKF9 | CPSF160 Q9FGR0 | 4 | EBI-1775444, EBI-1775436 | |
BINARY | Q9LKF9 | CPSF30 A9LNK9 | 3 | EBI-1775444, EBI-962511 | |
BINARY | Q9LKF9 | CPSF73-I Q9C952 | 4 | EBI-1775444, EBI-1775464 | |
BINARY | Q9LKF9 | CPSF73-II Q8GUU3 | 3 | EBI-1775444, EBI-1775477 | |
BINARY | Q9LKF9 | FY Q6NLV4 | 3 | EBI-1775444, EBI-1632908 | |
BINARY | Q9LKF9 | PAPS2 O82312 | 5 | EBI-1775444, EBI-1775513 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 411-430 | Disordered | ||||
Sequence: VKEEETKASHGSDDNSSEPM |
Sequence similarities
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length739
- Mass (Da)82,139
- Last updated2001-10-18 v2
- Checksum9ABFC916712AD464
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 49 | in Ref. 1; AAF82809 | ||||
Sequence: S → P | ||||||
Sequence conflict | 441 | in Ref. 1; AAF82809 | ||||
Sequence: I → V |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF283277 EMBL· GenBank· DDBJ | AAF82809.1 EMBL· GenBank· DDBJ | mRNA | ||
AB005244 EMBL· GenBank· DDBJ | BAB10061.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED93228.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY034982 EMBL· GenBank· DDBJ | AAK59487.1 EMBL· GenBank· DDBJ | mRNA | ||
BT004374 EMBL· GenBank· DDBJ | AAO42368.1 EMBL· GenBank· DDBJ | mRNA |