Q9KWL3 · LIGC_SPHSK

Function

function

Involved in the degradation of protocatechuate (PCA) via the PCA 4,5-cleavage pathway. Catalyzes the oxidation of the hemiacetal form of 4-carboxy-2-hydroxymuconate-6-semialdehyde (CHMS) to produce 2-pyrone-4,6-dicarboxylate (PDC). LigC has 10-times-higher affinity to NADP than to NAD.

Catalytic activity

Activity regulation

Inhibited by p-chloromercuribenzoate (10 mM), HgCl2 (10 mM), or 5,5-dithiobis(2-nitrobenzoate) (100 mM).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
20.6 μMNAD825with CHMS as substrate
24.6 μMCHMS825with NADP as cofactor
26 μMNADP825with CHMS as substrate
252 μMCHMS825with NAD as cofactor
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
363 μmol/min/mg825with CHMS as substrate, with NADP as cofactor
449 μmol/min/mg825with CHMS as substrate, with NAD as cofactor

pH Dependence

Optimum pH is 8.

Temperature Dependence

Optimum temperature is 25 degrees Celsius.

Pathway

Secondary metabolite metabolism; lignin degradation.

GO annotations

AspectTerm
Molecular Function4-carboxy-2-hydroxymuconate semialdehyde hemiacetal dehydrogenase activity
Molecular Functionnucleotide binding
Biological Process3,4-dihydroxybenzoate catabolic process
Biological Processlignin catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase
  • EC number
  • Short names
    CHMS dehydrogenase
  • Alternative names
    • 2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase

Gene names

    • Name
      ligC

Organism names

Accessions

  • Primary accession
    Q9KWL3

Phenotypes & Variants

Disruption phenotype

Disruption of this gene prevents growth with vanillate. Only PCA is accumulated during the incubation of vanillate with the whole cells of the ligC insertion mutant. A repression of PCA 4,5-dioxygenase (LigAB) activity is also observed.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004186161-3154-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the Gfo/Idh/MocA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    315
  • Mass (Da)
    34,629
  • Last updated
    2000-10-01 v1
  • Checksum
    58077E4EE667DCE1
MRIALAGAGAFGEKHLDGLKNIDGVEIVSIISRKAEQAAEVAAKYGAKHSGTDLSEALARDDVDAVILCTPTQMHAEQAIACMNAGKHVQVEIPLADSWADAEAVMKKSQETGLVCMVGHTRRFNPSHQYIHNKIVAGELAIQQMDVQTYFFRRKNMNAKGEPRSWTDHLLWHHAAHTVDLFAYQAGKIVQANAVQGPIHPELGIAMDMSIQLKSETGAICTLSLSFNNDGPLGTFFRYICDNGTWIARYDDLVTGKEEPVDVSKVDVSMNGIELQDREFIAAIREGREPNSSVARVLDCYRVLGELEVQLEKQG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB035122
EMBL· GenBank· DDBJ
BAA97119.1
EMBL· GenBank· DDBJ
Genomic DNA
AB073227
EMBL· GenBank· DDBJ
BAB88744.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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