Q9KWL3 · LIGC_SPHSK
- Protein4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase
- GeneligC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids315 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the degradation of protocatechuate (PCA) via the PCA 4,5-cleavage pathway. Catalyzes the oxidation of the hemiacetal form of 4-carboxy-2-hydroxymuconate-6-semialdehyde (CHMS) to produce 2-pyrone-4,6-dicarboxylate (PDC). LigC has 10-times-higher affinity to NADP than to NAD.
Catalytic activity
- 4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP+ = 2-oxo-2H-pyran-4,6-dicarboxylate + H+ + NADPH
Activity regulation
Inhibited by p-chloromercuribenzoate (10 mM), HgCl2 (10 mM), or 5,5-dithiobis(2-nitrobenzoate) (100 mM).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
20.6 μM | NAD | 8 | 25 | with CHMS as substrate | ||
24.6 μM | CHMS | 8 | 25 | with NADP as cofactor | ||
26 μM | NADP | 8 | 25 | with CHMS as substrate | ||
252 μM | CHMS | 8 | 25 | with NAD as cofactor |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
363 μmol/min/mg | 8 | 25 | with CHMS as substrate, with NADP as cofactor | ||
449 μmol/min/mg | 8 | 25 | with CHMS as substrate, with NAD as cofactor |
pH Dependence
Optimum pH is 8.
Temperature Dependence
Optimum temperature is 25 degrees Celsius.
Pathway
Secondary metabolite metabolism; lignin degradation.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4-carboxy-2-hydroxymuconate semialdehyde hemiacetal dehydrogenase activity | |
Molecular Function | nucleotide binding | |
Biological Process | 3,4-dihydroxybenzoate catabolic process | |
Biological Process | lignin catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase
- EC number
- Short namesCHMS dehydrogenase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingobium
Accessions
- Primary accessionQ9KWL3
Phenotypes & Variants
Disruption phenotype
Disruption of this gene prevents growth with vanillate. Only PCA is accumulated during the incubation of vanillate with the whole cells of the ligC insertion mutant. A repression of PCA 4,5-dioxygenase (LigAB) activity is also observed.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418616 | 1-315 | 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase | |||
Sequence: MRIALAGAGAFGEKHLDGLKNIDGVEIVSIISRKAEQAAEVAAKYGAKHSGTDLSEALARDDVDAVILCTPTQMHAEQAIACMNAGKHVQVEIPLADSWADAEAVMKKSQETGLVCMVGHTRRFNPSHQYIHNKIVAGELAIQQMDVQTYFFRRKNMNAKGEPRSWTDHLLWHHAAHTVDLFAYQAGKIVQANAVQGPIHPELGIAMDMSIQLKSETGAICTLSLSFNNDGPLGTFFRYICDNGTWIARYDDLVTGKEEPVDVSKVDVSMNGIELQDREFIAAIREGREPNSSVARVLDCYRVLGELEVQLEKQG |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length315
- Mass (Da)34,629
- Last updated2000-10-01 v1
- Checksum58077E4EE667DCE1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB035122 EMBL· GenBank· DDBJ | BAA97119.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB073227 EMBL· GenBank· DDBJ | BAB88744.1 EMBL· GenBank· DDBJ | Genomic DNA |