Q9K0U5 · SYDND_NEIMB
- ProteinAspartate--tRNA(Asp/Asn) ligase
- GeneaspS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids602 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).
Catalytic activity
- tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Site | 30 | Important for tRNA non-discrimination | |||
Site | 81 | Important for tRNA non-discrimination | |||
Binding site | 172 | L-aspartate (UniProtKB | ChEBI) | |||
Binding site | 218 | L-aspartate (UniProtKB | ChEBI) | |||
Binding site | 218-220 | ATP (UniProtKB | ChEBI) | |||
Binding site | 227 | ATP (UniProtKB | ChEBI) | |||
Binding site | 457 | L-aspartate (UniProtKB | ChEBI) | |||
Binding site | 491 | ATP (UniProtKB | ChEBI) | |||
Binding site | 498 | L-aspartate (UniProtKB | ChEBI) | |||
Binding site | 543-546 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | aspartate-tRNA ligase activity | |
Molecular Function | aspartate-tRNA(Asn) ligase activity | |
Molecular Function | ATP binding | |
Molecular Function | nucleic acid binding | |
Biological Process | aspartyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAspartate--tRNA(Asp/Asn) ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Neisseriales > Neisseriaceae > Neisseria
Accessions
- Primary accessionQ9K0U5
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000110910 | 1-602 | Aspartate--tRNA(Asp/Asn) ligase | ||
Proteomic databases
Interaction
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 196-199 | Aspartate | |||
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length602
- Mass (Da)68,124
- Last updated2000-10-01 v1
- Checksum5DC8A016B0C13E3C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE002098 EMBL· GenBank· DDBJ | AAF40903.1 EMBL· GenBank· DDBJ | Genomic DNA |