Q9JM47 · CSEN_RAT
- ProteinCalsenilin
- GeneKcnip3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids256 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity).
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (By similarity).
May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of amyloid-beta formation (By similarity).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 175 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 177 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 179 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 181 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 186 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 223 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 225 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 227 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 234 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCalsenilin
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ9JM47
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Lipid-anchor
Note: Also membrane-bound, associated with the plasma membrane. In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 45-46 | Greatly reduces plasma membrane localization. | ||||
Sequence: CC → AA or SS |
PTM/Processing
Features
Showing features for chain, modified residue, cross-link, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000073817 | 1-256 | Calsenilin | |||
Sequence: MQRTKEAMKASDGSLLGDPGRIPLSKREGIKWQRPRFTRQALMRCCLIKWILSSAAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYSQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPLEHVERFFQKMDRNQDGVVTIDEFLETCQKDENIMSSMQLFENVI | ||||||
Modified residue | 14 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 26 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) | ||||
Sequence: K | ||||||
Lipidation | 45 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 46 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Modified residue | 60 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 63 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 90 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) | ||||
Sequence: K |
Post-translational modification
Palmitoylated. Palmitoylation enhances association with the plasma membrane.
Proteolytically cleaved by caspase-3.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Detected in brain cortex, thalamus, dentate gyrus and cerebellum (at protein level) (PubMed:16123112).
Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells
Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells
Gene expression databases
Interaction
Subunit
Binds to DNA as a homomultimer. Dimerization is induced by binding to calcium. Interacts with the C-terminus of PSEN1 and PSEN2 and with PSEN2 CTF subunit. Associates with KCN1. Component of heteromultimeric potassium channels (PubMed:16123112).
Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity).
Interacts with KCND2 and KCND3 (PubMed:10676964).
Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity).
Interacts with KCND2 and KCND3 (PubMed:10676964).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-22 | Disordered | ||||
Sequence: MQRTKEAMKASDGSLLGDPGRI | ||||||
Domain | 67-123 | EF-hand 1; degenerate | ||||
Sequence: LELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYSQFFP | ||||||
Domain | 126-161 | EF-hand 2 | ||||
Sequence: DATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRG | ||||||
Domain | 162-197 | EF-hand 3 | ||||
Sequence: TVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDM | ||||||
Domain | 210-245 | EF-hand 4 | ||||
Sequence: APLEHVERFFQKMDRNQDGVVTIDEFLETCQKDENI | ||||||
Region | 243-256 | Interaction with KCND2 | ||||
Sequence: ENIMSSMQLFENVI |
Sequence similarities
Belongs to the recoverin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length256
- Mass (Da)29,471
- Last updated2000-10-01 v1
- Checksum6DF71E1584C1E9A0
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0G2K6M5 | A0A0G2K6M5_RAT | Kcnip3 | 290 | ||
A0A140TAG1 | A0A140TAG1_RAT | Kcnip3 | 255 | ||
A0A8L2R519 | A0A8L2R519_RAT | Kcnip3 | 277 | ||
A0A8L2QQE1 | A0A8L2QQE1_RAT | Kcnip3 | 230 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 208 | in Ref. 2; AAG15382 | ||||
Sequence: E → K |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB043892 EMBL· GenBank· DDBJ | BAA96360.1 EMBL· GenBank· DDBJ | mRNA | ||
AF297118 EMBL· GenBank· DDBJ | AAG15382.1 EMBL· GenBank· DDBJ | mRNA |