Q9JM47 · CSEN_RAT

Function

function

Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity).
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (By similarity).
May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of amyloid-beta formation (By similarity).

Features

Showing features for binding site.

125620406080100120140160180200220240
TypeIDPosition(s)Description
Binding site175Ca2+ 1 (UniProtKB | ChEBI)
Binding site177Ca2+ 1 (UniProtKB | ChEBI)
Binding site179Ca2+ 1 (UniProtKB | ChEBI)
Binding site181Ca2+ 1 (UniProtKB | ChEBI)
Binding site186Ca2+ 1 (UniProtKB | ChEBI)
Binding site223Ca2+ 2 (UniProtKB | ChEBI)
Binding site225Ca2+ 2 (UniProtKB | ChEBI)
Binding site227Ca2+ 2 (UniProtKB | ChEBI)
Binding site234Ca2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentaxon
Cellular Componentaxon terminus
Cellular Componentcytosol
Cellular Componentdendrite
Cellular Componentendoplasmic reticulum
Cellular ComponentGolgi apparatus
Cellular Componentnucleus
Cellular Componentprotein-DNA complex
Cellular Componentvoltage-gated potassium channel complex
Molecular Functioncalcium ion binding
Molecular Functioncalcium-dependent protein binding
Molecular Functioncore promoter sequence-specific DNA binding
Molecular FunctionDNA binding
Molecular FunctionDNA-binding transcription repressor activity, RNA polymerase II-specific
Molecular Functionmagnesium ion binding
Molecular Functionmolecular function activator activity
Molecular Functionpotassium channel activity
Molecular Functionpotassium channel regulator activity
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Functionsequence-specific DNA binding
Molecular Functiontransmembrane transporter binding
Biological Processapoptotic process
Biological Processbehavioral response to pain
Biological Processintracellular protein transport
Biological Processnegative regulation of transcription by RNA polymerase II
Biological Processprotein localization to plasma membrane
Biological Processregulation of neuron apoptotic process
Biological Processregulation of potassium ion transmembrane transport
Biological Processresponse to magnesium ion
Biological Processresponse to pain
Biological Processsensory perception of pain

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Calsenilin
  • Alternative names
    • A-type potassium channel modulatory protein 3
    • DRE-antagonist modulator (DREAM)
    • Kv channel-interacting protein 3 (KChIP3)

Gene names

    • Name
      Kcnip3
    • Synonyms
      Csen, Dream, Kchip3

Organism names

  • Taxonomic identifier
  • Strain
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q9JM47

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Cell membrane
; Lipid-anchor
Endoplasmic reticulum
Golgi apparatus
Nucleus
Note: Also membrane-bound, associated with the plasma membrane. In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis45-46Greatly reduces plasma membrane localization.

PTM/Processing

Features

Showing features for chain, modified residue, cross-link, lipidation.

TypeIDPosition(s)Description
ChainPRO_00000738171-256Calsenilin
Modified residue14Phosphoserine
Cross-link26Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)
Lipidation45S-palmitoyl cysteine
Lipidation46S-palmitoyl cysteine
Modified residue60Phosphoserine
Modified residue63Phosphoserine
Cross-link90Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)

Post-translational modification

Palmitoylated. Palmitoylation enhances association with the plasma membrane.
Proteolytically cleaved by caspase-3.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in brain cortex, thalamus, dentate gyrus and cerebellum (at protein level) (PubMed:16123112).
Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells

Gene expression databases

Interaction

Subunit

Binds to DNA as a homomultimer. Dimerization is induced by binding to calcium. Interacts with the C-terminus of PSEN1 and PSEN2 and with PSEN2 CTF subunit. Associates with KCN1. Component of heteromultimeric potassium channels (PubMed:16123112).
Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity).
Interacts with KCND2 and KCND3 (PubMed:10676964).

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-22Disordered
Domain67-123EF-hand 1; degenerate
Domain126-161EF-hand 2
Domain162-197EF-hand 3
Domain210-245EF-hand 4
Region243-256Interaction with KCND2

Sequence similarities

Belongs to the recoverin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    256
  • Mass (Da)
    29,471
  • Last updated
    2000-10-01 v1
  • Checksum
    6DF71E1584C1E9A0
MQRTKEAMKASDGSLLGDPGRIPLSKREGIKWQRPRFTRQALMRCCLIKWILSSAAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYSQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPLEHVERFFQKMDRNQDGVVTIDEFLETCQKDENIMSSMQLFENVI

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0G2K6M5A0A0G2K6M5_RATKcnip3290
A0A140TAG1A0A140TAG1_RATKcnip3255
A0A8L2R519A0A8L2R519_RATKcnip3277
A0A8L2QQE1A0A8L2QQE1_RATKcnip3230

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict208in Ref. 2; AAG15382

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB043892
EMBL· GenBank· DDBJ
BAA96360.1
EMBL· GenBank· DDBJ
mRNA
AF297118
EMBL· GenBank· DDBJ
AAG15382.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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