Q9JLI6 · SCLY_MOUSE
- ProteinSelenocysteine lyase
- GeneScly
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids432 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium.
Catalytic activity
- L-selenocysteine + AH2 = hydrogenselenide + L-alanine + A + H+
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
9.9 mM | L-selenocysteine | |||||
8.6 mM | L-cysteine sulfinate | |||||
5.2 mM | L-cysteine |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
58 μmol/min/mg | with L-selenocysteine as substrate | ||||
0.45 μmol/min/mg | with L-cysteine sulfinate as substrate | ||||
0.0074 μmol/min/mg | with L-cysteine as substrate |
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 375 | S-selanylcysteine intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | selenocysteine lyase activity | |
Molecular Function | transferase activity | |
Biological Process | lipid metabolic process | |
Biological Process | negative regulation of cellular response to oxidative stress | |
Biological Process | response to insulin | |
Biological Process | selenium compound metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSelenocysteine lyase
- EC number
- Short namesmSCL
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9JLI6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000317013 | 1-432 | Selenocysteine lyase | |||
Sequence: MDAARNGALGSVESLPDRKVYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVSGRKAKDIINAARASLAKMIGGKPQDIIFTSGGTESNNLVIHSMVRCFHEQQTLKGNMVDQHSPEEGTRPHFITCTVEHDSIRLPLEHLVENQMAEVTFVPVSKVNGQAEVEDILAAVRPTTCLVTIMLANNETGVIMPVSEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPRIGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERLPNTCNFSIQGSQLQGYTVLAQCRTLLASVGASCHSNHEDRPSPVLLSCGIPVDVARNAVRLSVGRGTTRADVDLIVQDLKQAVAQLEGRL | ||||||
Modified residue | 117 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 247 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed. Present in liver, kidney, testis (at protein level).
Structure
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9JLI6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length432
- Mass (Da)47,174
- Last updated2000-10-01 v1
- ChecksumAFC1800A1BC349D4
Q9JLI6-2
- Name2
- Differences from canonical
- 284-432: Missing
Computationally mapped potential isoform sequences
There are 7 potential isoforms mapped to this entry
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 146 | in Ref. 2; BAC29465 | ||||
Sequence: N → S | ||||||
Sequence conflict | 278 | in Ref. 2; BAC29465/BAC29563 | ||||
Sequence: R → W | ||||||
Alternative sequence | VSP_030855 | 284-432 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF175407 EMBL· GenBank· DDBJ | AAF36812.1 EMBL· GenBank· DDBJ | mRNA | ||
AK036533 EMBL· GenBank· DDBJ | BAC29465.1 EMBL· GenBank· DDBJ | mRNA | ||
AK036750 EMBL· GenBank· DDBJ | BAC29563.1 EMBL· GenBank· DDBJ | mRNA | ||
BC019879 EMBL· GenBank· DDBJ | AAH19879.1 EMBL· GenBank· DDBJ | mRNA | ||
BC021389 EMBL· GenBank· DDBJ | AAH21389.1 EMBL· GenBank· DDBJ | mRNA |