Q9JI93 · A4GAT_RAT

  • Protein
    Lactosylceramide 4-alpha-galactosyltransferase
  • Gene
    A4galt
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the transfer of galactose from UDP-alpha-D-galactose to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)) (PubMed:10854428).
Also able to transfer galactose to galactosylceramide/beta-D-Gal-(1<->1')-Cer (By similarity).
Globoside Gb3Cer is a glycosphingolipid of the globo serie, one of the major types of neutral root structures of glycosphingolipids, that constitute a significant portion of mammalian cell membranes (By similarity).

Catalytic activity

Pathway

Glycolipid biosynthesis.

GO annotations

AspectTerm
Cellular ComponentGolgi membrane
Cellular Componentmembrane
Molecular Functionacetylglucosaminyltransferase activity
Molecular Functiongalactosyltransferase activity
Molecular Functionlactosylceramide 4-alpha-galactosyltransferase activity
Molecular Functiontoxic substance binding
Biological Processgloboside biosynthetic process
Biological Processplasma membrane organization
Biological Processprotein O-linked glycosylation

Keywords

Enzyme and pathway databases

Protein family/group databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    Lactosylceramide 4-alpha-galactosyltransferase
  • EC number
  • Alternative names
    • Alpha-1,4-N-acetylglucosaminyltransferase
    • Alpha-1,4-galactosyltransferase
    • Alpha4Gal-T1
    • Globotriaosylceramide synthase (Gb3 synthase)
    • UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase

Gene names

    • Name
      A4galt

Organism names

  • Taxonomic identifier
  • Strain
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q9JI93
  • Secondary accessions
    • Q4QR98

Proteomes

Organism-specific databases

Subcellular Location

Golgi apparatus membrane
; Single-pass type II membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-30Cytoplasmic
Transmembrane31-51Helical; Signal-anchor for type II membrane protein
Topological domain52-360Lumenal

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis199-201Loss of activity.

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00000805821-360Lactosylceramide 4-alpha-galactosyltransferase
Glycosylation210N-linked (GlcNAc...) asparagine
Glycosylation316N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Ubiquitous. Highly expressed in kidney, mesenteric lymph node, spleen and brain.

Gene expression databases

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif199-201DXD motif

Domain

The conserved DXD motif is involved in enzyme activity.

Sequence similarities

Belongs to the glycosyltransferase 32 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    360
  • Mass (Da)
    41,551
  • Last updated
    2000-10-01 v1
  • Checksum
    0A4181886DB49D6A
MGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF248544
EMBL· GenBank· DDBJ
AAF82758.1
EMBL· GenBank· DDBJ
mRNA
BC097323
EMBL· GenBank· DDBJ
AAH97323.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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