Q9JI93 · A4GAT_RAT
- ProteinLactosylceramide 4-alpha-galactosyltransferase
- GeneA4galt
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids360 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the transfer of galactose from UDP-alpha-D-galactose to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)) (PubMed:10854428).
Also able to transfer galactose to galactosylceramide/beta-D-Gal-(1<->1')-Cer (By similarity).
Globoside Gb3Cer is a glycosphingolipid of the globo serie, one of the major types of neutral root structures of glycosphingolipids, that constitute a significant portion of mammalian cell membranes (By similarity).
Also able to transfer galactose to galactosylceramide/beta-D-Gal-(1<->1')-Cer (By similarity).
Globoside Gb3Cer is a glycosphingolipid of the globo serie, one of the major types of neutral root structures of glycosphingolipids, that constitute a significant portion of mammalian cell membranes (By similarity).
Catalytic activity
- a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP-alpha-D-galactose = a globoside Gb3Cer (d18:1(4E)) + H+ + UDPThis reaction proceeds in the forward direction.
- beta-D-Gal-(1<->1')-Cer + UDP-alpha-D-galactose = alpha-D-Gal-(1->4)-beta-D-Gal-(1<->1')-Cer + H+ + UDPThis reaction proceeds in the forward direction.
Pathway
Glycolipid biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Cellular Component | membrane | |
Molecular Function | acetylglucosaminyltransferase activity | |
Molecular Function | galactosyltransferase activity | |
Molecular Function | lactosylceramide 4-alpha-galactosyltransferase activity | |
Molecular Function | toxic substance binding | |
Biological Process | globoside biosynthetic process | |
Biological Process | plasma membrane organization | |
Biological Process | protein O-linked glycosylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameLactosylceramide 4-alpha-galactosyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ9JI93
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-30 | Cytoplasmic | ||||
Sequence: MGISRSDLEETMSKPPDCLPRMLRGTPRQR | ||||||
Transmembrane | 31-51 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: VFTLFIISFKFTFLVSILIYW | ||||||
Topological domain | 52-360 | Lumenal | ||||
Sequence: HTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 199-201 | Loss of activity. | ||||
Sequence: DTD → ATA |
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000080582 | 1-360 | Lactosylceramide 4-alpha-galactosyltransferase | |||
Sequence: MGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL | ||||||
Glycosylation | 210 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 316 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Ubiquitous. Highly expressed in kidney, mesenteric lymph node, spleen and brain.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 199-201 | DXD motif | ||||
Sequence: DTD |
Domain
The conserved DXD motif is involved in enzyme activity.
Sequence similarities
Belongs to the glycosyltransferase 32 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length360
- Mass (Da)41,551
- Last updated2000-10-01 v1
- Checksum0A4181886DB49D6A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF248544 EMBL· GenBank· DDBJ | AAF82758.1 EMBL· GenBank· DDBJ | mRNA | ||
BC097323 EMBL· GenBank· DDBJ | AAH97323.1 EMBL· GenBank· DDBJ | mRNA |