Q9JHS6 · ASIC4_RAT
- ProteinAcid-sensing ion channel 4
- GeneAsic4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids539 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Probable cation channel with high affinity for sodium.
Miscellaneous
In vitro, has no proton-gated channel activity.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | ligand-gated sodium channel activity | |
Biological Process | behavioral fear response | |
Biological Process | sodium ion transmembrane transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAcid-sensing ion channel 4
- Short namesASIC4
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ9JHS6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-68 | Cytoplasmic | ||||
Sequence: MPIEIVCKIKFAEEDAKPKEKEAGDEQSLLGAAQGPAAPRDLATFASTSTLHGLGRACGPGPHGLRRT | ||||||
Transmembrane | 69-89 | Helical | ||||
Sequence: LWVLALLTSLAAFLYQAASLA | ||||||
Topological domain | 90-438 | Extracellular | ||||
Sequence: RGYLTRPHLVAMDPAAPAPVAGFPAVTLCNINRFRHSALSDADIFHLANLTGLPPKDRDGHRAAGLRYPEPDMVDILNRTGHQLADMLKSCNFSGHHCSASNFSVVYTRYGKCYTFNADPQSSLPSRAGGMGSGLEIMLDIQQEEYLPIWRETNETSFEAGIRVQIHSQEEPPYIHQLGFGVSPGFQTFVSCQKQRLTYLPQPWGNCRAESKLREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPGNETICPPNIYIECADHTLDSLGGGSEGPCFCPTPCNLTRYGKEISMVKIPNRGSARYLARKYNRNETYIRENFLVLDVFFEALTSEAMEQRAAYGLSA | ||||||
Transmembrane | 439-459 | Helical | ||||
Sequence: LLGDLGGQMGLFIGASILTLL | ||||||
Topological domain | 460-539 | Cytoplasmic | ||||
Sequence: EILDYIYEVSWDRLKRVWRRPKTPLRTSTGGISTLGLQELKEQSPCPNRGRAEGGGASNLLPNHHHPHGPPGSLFEDFAC |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000181306 | 1-539 | Acid-sensing ion channel 4 | |||
Sequence: MPIEIVCKIKFAEEDAKPKEKEAGDEQSLLGAAQGPAAPRDLATFASTSTLHGLGRACGPGPHGLRRTLWVLALLTSLAAFLYQAASLARGYLTRPHLVAMDPAAPAPVAGFPAVTLCNINRFRHSALSDADIFHLANLTGLPPKDRDGHRAAGLRYPEPDMVDILNRTGHQLADMLKSCNFSGHHCSASNFSVVYTRYGKCYTFNADPQSSLPSRAGGMGSGLEIMLDIQQEEYLPIWRETNETSFEAGIRVQIHSQEEPPYIHQLGFGVSPGFQTFVSCQKQRLTYLPQPWGNCRAESKLREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPGNETICPPNIYIECADHTLDSLGGGSEGPCFCPTPCNLTRYGKEISMVKIPNRGSARYLARKYNRNETYIRENFLVLDVFFEALTSEAMEQRAAYGLSALLGDLGGQMGLFIGASILTLLEILDYIYEVSWDRLKRVWRRPKTPLRTSTGGISTLGLQELKEQSPCPNRGRAEGGGASNLLPNHHHPHGPPGSLFEDFAC | ||||||
Disulfide bond | 118↔202 | |||||
Sequence: CNINRFRHSALSDADIFHLANLTGLPPKDRDGHRAAGLRYPEPDMVDILNRTGHQLADMLKSCNFSGHHCSASNFSVVYTRYGKC | ||||||
Disulfide bond | 180↔187 | |||||
Sequence: CNFSGHHC | ||||||
Glycosylation | 191 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 243 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 296↔375 | |||||
Sequence: CRAESKLREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPGNETICPPNIYIECADHTLDSLGGGSEGPCFCPTPC | ||||||
Disulfide bond | 318↔371 | |||||
Sequence: CRLRCEKEAVLQRCHCRMVHMPGNETICPPNIYIECADHTLDSLGGGSEGPCFC | ||||||
Disulfide bond | 322↔369 | |||||
Sequence: CEKEAVLQRCHCRMVHMPGNETICPPNIYIECADHTLDSLGGGSEGPC | ||||||
Disulfide bond | 331↔353 | |||||
Sequence: CHCRMVHMPGNETICPPNIYIEC | ||||||
Disulfide bond | 333↔345 | |||||
Sequence: CRMVHMPGNETIC | ||||||
Glycosylation | 341 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 376 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in brain, spinal cord and dorsal root ganglion (DRG). Expressed by a subset of sensory neurons in the DRG. Expressed by granule cells in the cerebellar cortex. In hippocampus, expression is detected in dentate gyrus granule cells, in pyramidal cells of CA1-CA3 subfields and in interneurons of the striatum oriens and radiatum of all subfields. In cerebral cortex expressed in small, medium and large pyramidal cells in layers 2, 3 and 5 respectively. Also expressed in striatum, globus pallidus, inferior and superior calliculi, amygdala, magnocellular preoptic nucleus, islands of Calleja and large neurons of olfactory tubercules.
Developmental stage
Highly expressed in newborn spinal cord but hardly detected in the cerebellum compared to adult. Expressed at postnatal day 1 in ependymal cells lining the central canal of spinal cord and in motor neurons. In adult, expression decreases in ependymal cells and increases in motor neurons. The number of positive interneurons decreases but the individual interneuron expression increases in adult spinal cord compared to newborn.
Interaction
Subunit
Homotrimer or heterotrimer with other ASIC proteins.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 501-531 | Disordered | ||||
Sequence: EQSPCPNRGRAEGGGASNLLPNHHHPHGPPG |
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC4 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length539
- Mass (Da)59,325
- Last updated2000-10-01 v1
- Checksum3D73DE0EAAFF7097
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6AKV8 | A0A8I6AKV8_RAT | Asic4 | 520 | ||
G3V8N2 | G3V8N2_RAT | Asic4 | 539 | ||
A0A8I5ZSF3 | A0A8I5ZSF3_RAT | Asic4 | 526 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 283 | in Ref. 2; CAB61836 | ||||
Sequence: K → E | ||||||
Sequence conflict | 401 | in Ref. 2; CAB61836 | ||||
Sequence: K → R | ||||||
Sequence conflict | 536 | in Ref. 2; CAB61836 | ||||
Sequence: D → N |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ271642 EMBL· GenBank· DDBJ | CAB93984.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ242554 EMBL· GenBank· DDBJ | CAB61836.1 EMBL· GenBank· DDBJ | mRNA |